The current status of surgical pilonidal sinus disease therapy in Germany

European Surgery - Treatment of pilonidal sinus disease (PSD) requires a tailored approach. A national guideline was published in 2014. The current status of surgical PSD therapy...     

石棉相关肿瘤p53基因突变的免疫组化及PCR—SSCP研究

为了分析石棉相关肿瘤ｐ５３基因的突变特点，对石蜡包埋的石棉相关肿瘤ｐ５３基因突变体蛋白的表达进行了免疫组化观察，提取染色体ＤＮＡ，对ｐ５３基因的第５、７、８外显子进行ＰＣＲ－ＳＳＣＰ及测序分析。免疫组化观察发现：在分析的１０例病例中有５例阳性。ＰＣＲ－ＳＳＣＰ分析发现７例（８处）发生突变，其中４处集中在第８外显子上。５例腺癌中有４例发生ｐ５３基因突变。测序发现热点区突变。提示：石棉相关肿瘤ｐ５３基因突变率高。     

硬脊膜的蠕变率与椎间盘突出症的自然病程

目的　为探讨椎间盘突出症缓慢自愈的机理和治疗方法的选择提供一定的帮助。方法　应用２１ 例新鲜的猪的腰段硬脊膜,分组测量其蠕变率的变化。结果　各组蠕变率均与时间和载荷成正比,控温组发生速度快而大,模拟组发生慢而小。结论　蠕变率是椎间盘突出症症状缓解的力学基础,促进蠕变率发生的治疗,同减压治疗一样,都是病因治疗。     

机械通气在亚低温治疗重型颅脑损伤患者中的应用

目的：探讨机械通气对亚低温治疗重型颅脑损伤的影响。方法：对急性重型颅脑损伤患者在亚低温治疗中采用机械通气(机械通气组)，对比机械通气前后患者各项血气分析指标、达到亚低温治疗温度所需时间及颅内压的变化，并与32例正常呼吸组比较。完全机械通气组100例患者以肌松剂阻断了自主呼吸。以呼吸机行完全机械通气；不完全机械通气组68例保留自主呼吸。以同步呼吸模式辅助呼吸。结果：在应用呼吸机后。患者呼吸功能明显改善，血气分析指标基本恢复正常。达到亚低温所需治疗温度的时间显著短于对照组。2组机械通气治疗的患者颅内压明显降低。结论：机械通气改善了重型颅脑损伤患者的通气功能障碍。迅速降低颅内压。减少了继发脑损害。     

Antibiotic Susceptibility Profile of Bacteria Isolated from Natural Sources of Water from Rural Areas of East Sikkim

Background:

Contamination of water, food, and environment with antibiotic-resistant bacteria poses a serious public health issue.

Objective:

The objective was to study the bacterial pollution of the natural sources of water in east Sikkim and to determine the antimicrobial profile of the bacterial isolates.

Materials and Methods:

A total of 225 samples, 75 each during winter, summer, and monsoon season were collected from the same source in every season for bacteriological analysis by membrane filtration method. Antibiotic susceptibility test was performed using standard disc diffusion method.

Results:

A total of 19 bacterial species of the genera Escherichia, Klebsiella, Proteus, Salmonella, Shigella, Enterobacter, Citrobacter, Morganella, Pseudomonas, Acinetobacter, Flavobacterium, and Serratia were isolated and their antimicrobial sensitivity tested. Generally, most bacterial isolates except Salmonella and Shigella species were found resistant to commonly used antibiotics such as ampicillin (57.5%), trimethoprim/sulfamethoxaole (39.1%), amoxicillin/clavulanic acid (37.4%), cefixime (34.5%), tetracycline (29.1%), ceftazidime (26.3%), ofloxacin (25.9%), amikacin (8.7%), and gentamicin (2.7%) but sensitive to imipenem and piperacillin/tazobactam.

Conclusion:

Natural sources of water in east Sikkim are grossly contaminated with bacteria including enteropathogens. The consumption of untreated water from these sources might pose health risk to consumers.