全文获取类型
收费全文 | 425篇 |
免费 | 21篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 22篇 |
妇产科学 | 6篇 |
基础医学 | 67篇 |
口腔科学 | 14篇 |
临床医学 | 33篇 |
内科学 | 63篇 |
皮肤病学 | 11篇 |
神经病学 | 20篇 |
特种医学 | 11篇 |
外科学 | 77篇 |
综合类 | 5篇 |
预防医学 | 22篇 |
眼科学 | 6篇 |
药学 | 56篇 |
中国医学 | 1篇 |
肿瘤学 | 29篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 11篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 8篇 |
2017年 | 5篇 |
2016年 | 10篇 |
2015年 | 12篇 |
2014年 | 13篇 |
2013年 | 23篇 |
2012年 | 38篇 |
2011年 | 47篇 |
2010年 | 18篇 |
2009年 | 13篇 |
2008年 | 25篇 |
2007年 | 17篇 |
2006年 | 30篇 |
2005年 | 19篇 |
2004年 | 20篇 |
2003年 | 21篇 |
2002年 | 16篇 |
2001年 | 3篇 |
2000年 | 9篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1992年 | 9篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1984年 | 4篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1972年 | 2篇 |
1970年 | 2篇 |
1966年 | 3篇 |
1942年 | 1篇 |
1940年 | 1篇 |
1937年 | 1篇 |
1936年 | 1篇 |
1933年 | 1篇 |
1931年 | 1篇 |
1928年 | 1篇 |
1926年 | 1篇 |
排序方式: 共有447条查询结果,搜索用时 772 毫秒
1.
2.
The ouabain-resistant mutant cell lines, HOA-1 and HOA-20 were developed from WI-L2-729-HF2 by cloning with increasing concentration of ouabain. Both parent and mutant cell lines were resistant to base analogues, 6-thioguanine (6-TG) and 8-azaguanine (8-AG) to the level of 20 micrograms/ml in the culture medium. The parent cell line WI-L2-729-HF2 was highly sensitive to ouabain, whereas HOA-1 and HOA-20 were resistant to ouabain to the level of 1 microM and 20 microM, respectively. However, all the cell lines were sensitive to HAT-selective medium which is essential for hybrid selection after fusion. All three lymphoblastoid cell lines were positive for Epstein-Barr virus nuclear antigen (EBNA), secreted TNF-beta (lymphotoxin) without any external stimulation, secreted trace amounts of IgG(kappa), which was also present in their cytoplasm and had IgM(kappa) as surface bound immunoglobulin. They also expressed the CD20, CD71 (transferrin receptor) as surface antigens. In addition to these antigens, HOA-20 also expressed CD38 antigen. The karyotype analysis of these cell lines revealed modal chromosomal numbers ranging from 40 to 47. The HLA-A, -B and -C antigens expressed by WI-L2-729-HF2 and its mutants HOA-1 and HOA-20 were identical. Both the HOA-1 and HOA-20 mutants were found suitable for the generation of hybrids after fusion with EBV-transformed human B-lymphocytes. 相似文献
3.
4.
5.
Phagosomal processing of Mycobacterium tuberculosis antigen 85B is modulated independently of mycobacterial viability and phagosome maturation
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ramachandra L Smialek JL Shank SS Convery M Boom WH Harding CV 《Infection and immunity》2005,73(2):1097-1105
Control of Mycobacterium tuberculosis infection requires CD4 T-cell responses and major histocompatibility complex class II (MHC-II) processing of M. tuberculosis antigens (Ags). We have previously demonstrated that macrophages process heat-killed (HK) M. tuberculosis more efficiently than live M. tuberculosis. These observations suggested that live M. tuberculosis may inhibit Ag processing by inhibiting phagosome maturation or that HK M. tuberculosis may be less resistant to Ag processing. In the present study we examined the correlation between M. tuberculosis viability and phagosome maturation and efficiency of Ag processing. Since heat treatment could render M. tuberculosis Ags more accessible to proteolysis, M. tuberculosis was additionally killed by antibiotic treatment and radiation. Processing of HK, live, radiation-killed (RadK), or rifampin-killed (RifK) M. tuberculosis in activated murine bone marrow macrophages was examined by using an I-A(b)-restricted T-cell hybridoma cell line (BB7) that recognizes an epitope derived from Ag 85B. Macrophages processed HK M. tuberculosis more rapidly and efficiently than they processed live, RadK, or RifK M. tuberculosis. Live, RadK, and RifK M. tuberculosis cells were processed with similar efficiencies for presentation to BB7 T hybridoma cells. Furthermore, phagosomes containing live or RadK M. tuberculosis expressed fewer M. tuberculosis peptide-MHC-II complexes than phagosomes containing HK M. tuberculosis expressed. Since only live M. tuberculosis was able to prevent acidification of the phagosome, our results suggest that regulation of phagosome maturation does not explain the differences in processing of different forms of M. tuberculosis. These findings suggest that the mechanisms used by M. tuberculosis to inhibit phagosomal maturation differ from the mechanisms involved in modulating phagosome Ag processing. 相似文献
6.
Fine needle aspiration cytology plays an important role in the preoperative assessment of soft tissue neoplasms. In a 40-year-old man presenting with a large soft tissue mass in the posterior aspect of thigh a diagnosis of myxoid liposarcoma was suggested on FNAC. Scrape smears of the excised mass showed an additional finding of round cell component. Histopathology confirmed combined myxoid and round cell liposarcoma (grade 2), which behaves aggressively when compared to pure myxoid liposarcoma. 相似文献
7.
8.
9.
10.
Kishanchand Chethan Seetharam Prasad Lingadakai Ramachandra 《The Canadian Journal of Infectious Diseases & Medical Microbiology》2012,23(3):e65-e66
Mucormycosis is a relatively uncommon, aggressive and lethal mycosis. Fungi from the order Mucorales are the etiological agents of mucormycosis. The condition is more common among the immunocompromised, diabetic patients with ketoacidosis and people with iron overload syndromes. Diagnosis of mucormycosis requires a high index of suspicion regarding the possibility of the condition in high-risk individuals. Timely diagnosis is critical to survival and minimization of morbidity. A favourable outcome is possible only if appropriate treatment is initiated as early as possible. The present article reports a case of ileocolic mucormycosis involving a patient with chronic renal failure and familial hyperuricemia. 相似文献