全文获取类型
收费全文 | 253篇 |
免费 | 11篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 40篇 |
儿科学 | 31篇 |
妇产科学 | 38篇 |
基础医学 | 11篇 |
口腔科学 | 12篇 |
临床医学 | 6篇 |
内科学 | 18篇 |
皮肤病学 | 6篇 |
神经病学 | 4篇 |
特种医学 | 15篇 |
外科学 | 43篇 |
预防医学 | 7篇 |
眼科学 | 5篇 |
药学 | 14篇 |
肿瘤学 | 15篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 13篇 |
2020年 | 4篇 |
2019年 | 6篇 |
2018年 | 4篇 |
2017年 | 7篇 |
2016年 | 8篇 |
2015年 | 7篇 |
2014年 | 7篇 |
2013年 | 16篇 |
2012年 | 16篇 |
2011年 | 14篇 |
2010年 | 8篇 |
2009年 | 9篇 |
2008年 | 21篇 |
2007年 | 21篇 |
2006年 | 21篇 |
2005年 | 26篇 |
2004年 | 15篇 |
2003年 | 14篇 |
2002年 | 12篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1993年 | 1篇 |
1983年 | 2篇 |
排序方式: 共有265条查询结果,搜索用时 31 毫秒
1.
Oocyte morphology does not affect fertilization rate, embryo quality and implantation rate after intracytoplasmic sperm injection 总被引:6,自引:10,他引:6
Balaban B; Urman B; Sertac A; Alatas C; Aksoy S; Mercan R 《Human reproduction (Oxford, England)》1998,13(12):3431-3433
In this study, we compared the fertilization rate and embryo quality after
intracytoplasmic sperm injection (ICSI) as they relate to oocyte
morphology. A total of 654 ICSI cycles yielding 5903 metaphase II oocytes
were observed. The oocytes retrieved in these cycles were divided into (i)
normal oocytes, (ii) oocytes with extracytoplasmic abnormalities (dark zona
pellucida and large perivitelline space), (iii) oocytes with cytoplasmic
abnormalities (dark cytoplasm, granular cytoplasm, and refractile body),
(iv) oocytes with shape abnormalities, and (v) oocytes with more than one
abnormality (double and triple abnormalities). Intracytoplasmic vacuoles
and aggregates of smooth endoplasmic reticulum were not recorded
separately. The fertilization rate and quality of morphologically graded
embryos did not differ between the groups. There were 77 cycles where all
transferred embryos were derived from abnormal oocytes, and 164 cycles
where all embryos were derived from normal oocytes. These cycles were
studied further. The two groups were comparable regarding mean female age,
duration of infertility, duration of ovarian stimulation, number of
ampoules of gonadotrophin injected, and number of oocytes retrieved. Two
clinical pregnancy rates (44.4 versus 42.1%) and implantation rates per
embryo (10.3 versus 13.2%) were similar. In conclusion, in couples
undergoing ICSI, abnormal oocyte morphology is not associated with a
decreased fertilization rate or unfavourable embryo quality. Furthermore,
embryos derived from abnormal oocytes yield similar clinical pregnancy and
implantation rates when transferred compared with embryos derived from
normal oocytes.
相似文献
2.
Meltem Dinleyici Vicente Prez-Brocal Sertac Arslanoglu Ozge Aydemir Sibel Sevuk Ozumut Neslihan Tekin Yvan Vandenplas Andrs Moya Ener Cagri Dinleyici 《Nutrients》2021,13(6)
The human milk (HM) microbiota is a significant source of microbes that colonize the infant gut early in life. The aim of this study was to compare transient and mature HM virome compositions, and also possible changes related to the mode of delivery, gestational age, and weight for gestational age. Overall, in the 81 samples analyzed in this study, reads matching bacteriophages accounted for 79.5% (mainly Podoviridae, Myoviridae, and Siphoviridae) of the reads, far more abundant than those classified as eukaryotic viruses (20.5%, mainly Herpesviridae). In the whole study group of transient human milk, the most abundant families were Podoviridae and Myoviridae. In mature human milk, Podoviridae decreased, and Siphoviridae became the most abundant family. Bacteriophages were predominant in transient HM samples (98.4% in the normal spontaneous vaginal delivery group, 92.1% in the premature group, 89.9% in the C-section group, and 68.3% in the large for gestational age group), except in the small for gestational age group (only ~45% bacteriophages in transient HM samples). Bacteriophages were also predominant in mature HM; however, they were lower in mature HM than in transient HM (71.7% in the normal spontaneous vaginal delivery group, 60.8% in the C-section group, 56% in the premature group, and 80.6% in the large for gestational age group). Bacteriophages still represented 45% of mature HM in the small for gestational age group. In the transient HM of the normal spontaneous vaginal delivery group, the most abundant family was Podoviridae; however, in mature HM, Podoviridae became less prominent than Siphoviridae. Myoviridae was predominant in both transient and mature HM in the premature group (all C-section), and Podoviridae was predominant in transient HM, while Siphoviridae and Herpesviridae were predominant in mature HM. In the small for gestational age group, the most abundant taxa in transient HM were the family Herpesviridae and a species of the genus Roseolovirus. Bacteriophages constituted the major component of the HM virome, and we showed changes regarding the lactation period, preterm birth, delivery mode, and birth weight. Early in life, the HM virome may influence the composition of an infant’s gut microbiome, which could have short- and long-term health implications. Further longitudinal mother–newborn pair studies are required to understand the effects of these variations on the composition of the HM and the infant gut virome. 相似文献
3.
Bulent Urman Cengiz Alatas Senai Aksoy Alp Nuhoglu Aycan Sertac Basak Balaban 《Journal of assisted reproduction and genetics》1998,15(3):125-128
Purpose:
Our purpose was to determine the feasibility and efficacy of performing testicular or epididymal sperm retrieval prior to the injection of human chorionic gonadotropin (hCG).
Methods:
This report deals with 87 sperm-positive percutaneous epididymal sperm aspiration (PESA), percutaneous testicular sperm aspiration (PTSA), or testicular sperm extraction (TESE) cycles. All sperm retrieval procedures were performed prior to administration of hCG to the women. Retrieved spermatozoa were cultured in vitro in simple medium for approximately 40 hr prior to intracyto-plasmic sperm injection.
Results:
In all but one cycle in which TESE was performed for nonobstructive azoospermia, motile sperm were available for ICSI. The overall fertilization rate was 53%. Pregnancy rate per transfer and implantation rate per embryo were 41.2 and 15.7%, respectively.
Conclusions:
Satisfactory fertilization and pregnancy rates can be achieved when PESA, PTSA, or TESE is performed prior to the injection of hCG followed by in vitro culture of spermatozoa approximately 40 hr before ICSI. Scheduling of testicular or epididymal sperm retrieval cases in this way appears to ease the workload on laboratory and operating room personnel. Furthermore, withholding hCG when sperm is absent may obviate the unnecessary risk of ovarian hyperstimulation when spermatozoa cannot be retrieved. 相似文献
4.
5.
Kevin L. Greason Juan A. Crestanello Katherine S. King Gabor Bagameri Sertac M. Cicek John M. Stulak Richard C. Daly Joseph A. Dearani Hartzell V. Schaff 《The Journal of thoracic and cardiovascular surgery》2021,161(1):12-20.e2
BackgroundThere is controversy regarding the extent of aortic resection necessary in patients with aortopathy related to bicuspid aortic valve disease. To address this issue, we reviewed our experience in patients undergoing ascending aorta replacement during bicuspid aortic valve replacement.MethodsWe reviewed 702 patients who underwent ascending aorta replacement at the time of initial nonemergent native bicuspid aortic valve replacement at our institution between January 2000 and June 2017. Treatment cohorts included an open hemiarch replacement group (n = 225; 32%) and a clamped ascending aorta replacement group (n = 477; 68%).ResultsMedian patient age was 60 years (interquartile range [IQR], 51-67 years), female sex was present in 113 patients (16%), ejection fraction was 62% (IQR, 56%-66%), and aortic arch diameter was 33 mm (IQR, 29-36 mm). Cardiopulmonary bypass time was longer in the hemiarch replacement group (188 minutes vs 97 minutes; P < .001). Procedure-related complications (36%) and mortality (<1%) were similar in the 2 groups; however, the hemiarch group had an increased odds of blood transfusion (odds ratio, 1.62; 95% confidence interval [CI], 1.15-2.28; P = .006). The median duration of follow-up was 6.0 years (95% CI, 5.3-6.8 years). Overall survival was 94 ± 1% at 5 years and 80 ± 2% at 10 years. Multivariable analysis demonstrated similar survival in the 2 groups (hazard ratio, 0.83; 95% CI, 0.51-1.33; P = .439). No repeat aortic arch operations were done for aortopathy over the duration of clinical follow-up.ConclusionsCompared with patients in the clamped ascending aorta replacement group, patients in the hemi-arch replacement group had longer cardiopulmonary bypass and aortic cross-clamp times, along with an increased risk of blood transfusion, but similar freedom from repeat aortic arch operation and survival. We identified no advantage of performing hemiarch replacement in the absence of aortic arch dilation. 相似文献
6.
Haslak Fatih Barut Kenan Durak Cansu Aliyeva Ayten Yildiz Mehmet Guliyeva Vafa Varol Sevki Erdem Cebeci Sinem Oral Aygun Fatih Varli Yusuf Ziya Ozel Abdulrahman Onan Sertac Hanedan Kocoglu Ulkem Erol Meltem Karagozlu Fatih Ulug Nujin Dedeoglu Reyhan Sahin Sezgin Adrovic Amra Oztunc Funda Kasapcopur Ozgur 《Clinical rheumatology》2021,40(10):4167-4178
Clinical Rheumatology - Multi-system inflammatory syndrome in children (MIS-C) is a less understood and a rare complication of coronavirus disease-2019 (COVID-19). Given the scarce data regarding... 相似文献
7.
8.
9.
Yusuf Hidir Fuat Tosun Mehmet Ali Saracli Armagan Gunal Mustafa Gulec Sertac Yetiser 《European archives of oto-rhino-laryngology》2008,265(4):415-419
The aim of this study is to investigate intranasal fungal incidence in Turkish patients with chronic rhinosinusitis (CRS).
One hundred patients with CRS and 40 healthy subjects as a control group were included in the prospective study. Nasal lavage
samples were investigated for the presence of fungus, using new culture techniques and histopathologic examination. Epidermal
and intradermal hypersensitivity tests were used to find out fungal atopy. Levels of serum eosinophils, total IgE and presence
of fungus-specific IgE were investigated in the blood samples. Quantitative parameters were analyzed using Pearson χ2 test. Allergic fungal sinusitis (AFS) was diagnosed in 13(13%) patients. There was no fungal growth in the control group.
Eosinophilic mucin was detected in 94% of patients. Clinical and laboratory parameters of CRS were not significantly different
in patient groups with or without intranasal fungi. As a result, fungal incidence was not as high as expected in Turkish patients
with CRS. Furthermore, no significant correlation could be found between presence of intranasal fungus and type-1 hypersensitivity
in patients with CRS. 相似文献
10.
Induction of apoptosis in tumor-associated endothelial cells and therapy of orthotopic human pancreatic carcinoma in nude mice
下载免费PDF全文
![点击此处可从《Neoplasia (New York, N.Y.)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yokoi K Kim SJ Thaker P Yazici S Nam DH He J Sasaki T Chiao PJ Sclabas GM Abbruzzese JL Hamilton SR Fidler IJ 《Neoplasia (New York, N.Y.)》2005,7(7):696-704
Although gemcitabine has been accepted as the first-line chemotherapeutic reagent for advanced pancreatic cancer, improvement of response rate and survival is not sufficient and patients often develop resistance. We hypothesized that the inhibition of phosphorylation of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor receptor (VEGFR) on tumor cells and tumor-associated endothelial cells, combined with gemcitabine, would overcome the resistance to gemcitabine in orthotopic pancreatic tumor animal model. L3.6pl, human pancreatic cancer cells growing in the pancreas, and tumor-associated endothelial cells in microorgan environment highly expressed phosphorylated EGFR, VEGFR, and Akt, which regulates antiapoptotic mechanism. Oral administration of AEE788 (dual tyrosine kinase inhibitor against EGFR and VEGFR) inhibited the phosphorylation of EGFR, VEGFR, and Akt on tumor-associated endothelial cells as well as tumor cells. Although intraperitoneal (i.p.) injection of gemcitabine showed limited inhibitory effect on tumor growth, combination with AEE788 and gemcitabine produced nearly 95% inhibition of tumor growth in parallel with a high level of apoptosis on tumor cells and tumor-associated endothelial cells, and decreased microvascular density and proliferation rate. Collectively, these data indicate that dual inhibition of phosphorylation of EGFR and VEGFR, in combination with gemcitabine, produces apoptosis of tumor-associated endothelial cells and significantly suppresses human pancreatic cancer in nude mice. 相似文献