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1.
Olle Zetterström Christer Andersson Leif Eriksson ers Fredriksson Johan Friskopp Gunnar Heden Bernt Jansson Tord Lundgren Rolf Nilveus ers Olsson Stefan Renvert Lars Salonen Lars Sjöström ers Winell ers Östgren Stina Gestrelius 《Journal of clinical periodontology》1997,24(9):697-704
Abstract The aim of the present clinical trial was to test tolerability during 2 treatments with EMDOGAIN® in a large number of patients. An open, controlled study design in 10 Swedish specialist clinics was chosen, with a test group of 107 patients treated with EMDOGAIN® in connection with periodontal surgery at 2 surgical test sites per patient. The procedures were performed 2 to 6 weeks apart on one-rooted teeth with at least 4 mm deep intraosseous lesions. A control group of 33 patients underwent flap surgery without EMDOGAIN® at I comparable site. In total 214 test and 33 control surgeries were performed. Serum samples were obtained from test patients for analysis of total and specific antibody levels. 10 of the patients had samples taken before and after the first surgery. 56 other samples were taken after one treatment with EMDOGAIN®, and 63 after 2 treatments. None of the samples, not even from allergy-prone patients after 2 treatments, indicated deviations from established baseline ranges. This indicates that the immunogenic potential of EMDOGAIN® is extremely low when applied in conjunction with periodontal surgery. Comparison between the test and control groups demonstrated the same type and frequency of post-surgical experiences, i.e., reactions caused by the surgical procedure itself. Clinical probing and radiographic evaluation was performed at baseline and 8 months postsurgery. About half of the patients (44 test and 21 control) were also evaluated after 3 years. There was a significant difference between the test and control results at 8 months post surgery. and this difference had increased further at the 3 year follow-up. The 2.5–3 mm increase in attachment and bone level after treatment with EMDOGAIN® was of the same magnitude as seen in the studies with split-mouth design aiming for lest of effectiveness of EMDOGAIN®. 相似文献
2.
A Vannas D F Sweeney B A Holden E Sapyska E M Salonen A Vaheri 《Current eye research》1992,11(3):243-251
Plasmin, a proteolytic enzyme, has been detected in the tears of patients experiencing anterior ocular disease, and during contact lens wear. Using a radial caseinolysis procedure, we examined tear plasmin levels in 66 patients who were wearing soft and rigid lenses for daily and extended wear. Compared to non-contact lens wearers, patients wearing soft and rigid lenses for extended wear were significantly more likely to exhibit tear plasmin activity. Eight hours of open-eye thick HEMA lens wear did not induce tear plasmin activity in a group of 10 subjects. However, significant increases in tear plasmin activity were recorded after short-term (1 hour) eye closure with and without lens wear, and following overnight (8 hours) eye closure without lens wear. Overnight eye closure also resulted in significantly increased numbers of epithelial cells and leucocytes in the tear fluid. Our results suggest that increased tear plasmin activity during extended contact lens wear may be attributable to the effects of eye closure rather than hypoxia or the presence of the contact lens per se. 相似文献
3.
Retinal pigment epithelial cells secrete urokinase-type plasminogen activator and its inhibitor PAI-1. 总被引:3,自引:0,他引:3
V Sirén R W Stephens E M Salonen A Vaheri P Summanen I Immonen 《Ophthalmic research》1992,24(4):203-212
Secretion of plasminogen activators and their inhibitors was examined in cultures of human retinal pigment epithelial (RPE) cells. The methods employed were zymography and reverse zymography, solid-phase immunocapture assay, metabolic labeling followed by immunoprecipitation, and immunofluorescence. The results showed that these cells produce urokinase-type plasminogen activator (u-PA) and a plasminogen activator inhibitor (PAI) which is immunologically and biochemically similar to PAI-1. Tissue-type plasminogen activator activity (t-PA) was not detected, but we detected small amounts of t-PA in an inactive complex with inhibitor in RPE cell-conditioned media. We conclude that RPE cells have the potential to utilize u-PA-catalyzed plasminogen activation which is subject to regulation by PAI-1. These results may have a bearing on the pathogenesis of proliferative retinal diseases. 相似文献
4.
Depression-executive dysfunction syndrome in stroke patients. 总被引:6,自引:0,他引:6
Risto Vataja Tarja Pohjasvaara Riitta M?ntyl? Raija Ylikoski Maarit Leskel? Hely Kalska Marja Hietanen Hannu Juhani Aronen Oili Salonen Markku Kaste Antero Lepp?vuori Timo Erkinjuntti 《The American journal of geriatric psychiatry》2005,13(2):99-107
OBJECTIVE: It has been suggested that executive dysfunction could be the core defect in patients with geriatric or vascular depression, and that this depression-dysexecutive syndrome (DES) might be related to frontal-subcortical circuit dysfunction. The authors tested this hypothesis in 158 poststroke patients, of whom 21 had both depression and executive dysfunction. Methods: In this cross-sectional cohort study, a neurological, psychiatric, and neuropsychological examination was carried out 3 months after ischemic stroke, and brain infarcts, white-matter changes, and brain atrophy were recorded by MRI. RESULTS: The 21 patients with DES had significantly more brain infarcts affecting their frontal-subcortical circuit structures than the 137 patients without DES, or the 41 patients with depression but without executive dysfunction. Patients with DES also had more severe depressive symptoms and worse psychosocial functioning, and they coped less well in complex activities of daily living. CONCLUSIONS: DES is a valid concept and may define a subgroup of poststroke patients with frontal-subcortical pathology and with distinct prognosis and treatment options. 相似文献
5.
SUMMARY Porcelain fused to metal provides better aesthetics in fixed partial dentures than veneers with gold‐resin that were used formerly. The aim of our study was to evaluate complications and primary failures of fixed metal ceramic bridge prostheses made by dental students. We studied 61 patients (32 women, 29 men, mean age 49 years, range 28–73 years) treated during years 1990–1993. Data were collected from the patient files. Altogether 82 bridges were made (mean 4·1 units, range 2–6), 221 abutments (mean 2·7, range 2–6) and 136 pontics (mean 1·6, range 1–4). Forty‐seven cast cores were used in 29 bridges (mean 0·4 cores, range 1–3) and semiprecious attachments as an extra attachment in two bridges. Seven teeth were extracted due to complication and/or failure during endodontic treatment and root canal perforation during preparation. In two cases the abutment tooth was fractured by removing the old crown. Four unsuccessful bridges were remade and in seven cases the firing of porcelain was renewed. The study concludes that most common failures of fixed metal ceramic bridges made by dental students occur during root canal preparation of abutment teeth. 相似文献
6.
Biotransformation of medetomidine in the rat 总被引:1,自引:0,他引:1
J S Salonen M Eloranta 《Xenobiotica; the fate of foreign compounds in biological systems》1990,20(5):471-480
1. The metabolites of a novel alpha 2-adrenoceptor agonist, medetomidine, in rat urine after subcutaneous administration at two dose levels (80 micrograms/kg or 5 mg/kg), and after incubation with rat liver fractions, were characterized by h.p.l.c., 1H-n.m.r and mass spectrometry. 2. Hydroxylation of a methyl substituent was the main biotransformation in vitro. Hydroxylation occurred at a rate sufficient for high metabolic clearance. 3. The major urinary metabolites were the glucuronide of hydroxymedetomidine (about 35% of urinary metabolites) and medetomidine carboxylic acid (about 40%). 4. Medetomidine unchanged represented about 1% or 10% of the urinary excretion products, dependent on dose. 5. A metabolic pathway consisting of hydroxylation with subsequent glucuronidation, or further oxidation to carboxylic acid, is suggested. 相似文献
7.
Akbar F Heinonen S Pirskanen M Uimari P Tuomainen TP Salonen JT 《Molecular human reproduction》2005,11(1):73-77
Association between pre-eclampsia (PEE1) and the dimethylarginine dimethylaminohydrolase (DDAH) 1 and 2 genes, which play a role in the regulation of nitric oxide synthesis and release, was studied. In a case-control study design single nucleotide polymorphisms (SNPs) were determined at eight sites in the DDAH1 gene and at one site (Pro231Pro) in the DDAH2 gene from 132 women with pre-eclampsia and 112 healthy controls. Three SNPs in the DDAH1 gene were associated with pre-eclampsia, showing complete linkage disequilibrium with each other, but none of the associations in the allele or genotype data reached statistical significance in either of the genes after the correction for multiple testing. Haplotype frequencies were estimated using a population based on a maximum likelihood method (EM algorithm). Four common DDAH1 haplotypes were present and a significant association of haplotypes H2 and H3 with pre-eclampsia (P=0.03) was found. The risk of pre-eclampsia was greatest in individuals (odds ratio: 3.93; 95% confidence interval: 1.54-9.99) who had two copies of the high-risk haplotypes (H2 or H3). The observed haplotypic association provides the first evidence of the importance of DDAH1 polymorphisms in pre-eclampsia susceptibility. 相似文献
8.
Measles virus inhibits lymphocyte proliferation in vitro by two different mechanisms. 总被引:2,自引:0,他引:2 下载免费PDF全文
We studied the effect of two different strains of measles virus (MV) on the lymphocyte blast transformation response. Infectious virus inhibited the proliferation response to PHA early during the culture when freshly isolated PBMC were stimulated. However, MV stimulated the proliferation of T cell lines early after infection and the inhibition was a late phenomenon associated with cell death. The early inhibition by the Edmonston strain of MV was shown to be monocyte-dependent by depletion studies with monoclonal antibodies. It could be partially explained by IFN-alpha production as the addition of anti-IFN-alpha antiserum to the cultures reversed the virus-induced inhibition and the addition of IFN reduced the response of uninfected PBMC. The late inhibition by the Edmonston strain was associated neither with monocytes nor with IFN-alpha but correlated with cell death in cultures. The inhibition by the Halle strain of MV was strong and associated with cell death already early after infection. The results demonstrate that MV inhibits lymphocyte proliferation by two different mechanisms and different virus strains vary both in the magnitude and mechanism of inhibition. 相似文献
9.
The anxiolytic profile of dexmedetomidine, a novel, highly-selective 2-adrenergic agonist, was examined in rats in the elevated plus-maze test when administered either alone or in combination with the benzodiazepine agonist midazolam. Dexmedetomidine, 0.1–10 µg/kg, was inactive in modifying the rats' behavioral response in this test. Midazolam, 0.1–10 mg/kg, dose-dependently produced an anxiolytic-like profile characterized by an increased time spent in the open arms of the elevated plus-maze. A combination of dexmedetomidine 0.5 µg/kg and midazolam 0.5 mg/kg produced a synergistic interaction. This heterergic interaction of dexmedetomidine on midazolam's anxiolytic-like profile was dose-dependently blocked by pretreatment with an 2-adrenergic antagonist, atipamezole, 10–50 µg/kg, and a benzodiazepine antagonist flumazenil, 1.0 and 10 mg/kg, but not by the 1-adrenergic antagonist, prazosin, 0.1–10 mg/kg. While the transmembrane signal transduction pathways for benzodiazepine- and 2-agonist responses do not share any molecular component, there does appear to be crosstalk between these two systems. These may involve GABA or noradrenergic downstream effects of either dexmedetomidine or midazolam, respectively. 相似文献
10.