小鼠阴道黏膜HSV-2感染细胞凋亡与Fas Bcl-2的关系

目的探讨单纯疱疹病毒2型(herpes simp lex virus type 2,HSV-2)直接感染诱导小鼠阴道黏膜的细胞凋亡情况与Fas,Bc l-2蛋白水平的关系。方法建立HSV-2阴道感染小鼠模型。用TUNEL染色研究HSV-2诱导阴道上皮细胞凋亡,检测Fas,Bc l-2蛋白的水平,分析其与细胞凋亡的相关关系。结果22份标本有凋亡细胞阳性表达,并同时伴有炎症及坏死。Fas蛋白阳性率高(24/24),Bc l-2蛋白阳性率低(2/24)。结论HSV-2直接感染小鼠阴道后可同时诱导阴道上皮细胞凋亡及炎症坏死,Fas蛋白呈高水平,与凋亡细胞指数呈正相关(r=0.9660,P<0.05),提示Fas对HSV-2诱导的黏膜细胞凋亡起上调作用。     

酒舒颗粒的提取工艺研究

目的 确定酒舒颗粒超声提取的最佳工艺条件.方法 以总黄酮提取率为指标,采用单因素试验和正交试验考察提取温度、提取时间、提取次数和料液比对酒舒颗粒超声提取工艺的影响.结果 最佳提取工艺条件为提取温度70℃,提取时间1h,提取次数2次,料液比1∶8.最佳提取工艺条件下酒舒颗粒中总黄酮提取率为7.56％.结论 优选的提取工艺设计合理,可作为酒舒颗粒提取生产工艺的参考.     

动态监测C-反应蛋白水平在创伤手术中的应用价值

目的动态监测血清中C-反应蛋白（C-reactive protein，CRP）水平，探讨其在创伤手术患者抗感染治疗中的临床应用价值。方法随机选择100例创伤手术患者，用NycoCard ReaderⅡ定量金标检测仪，检测患者在创伤手术后1d内、3d、5d、7d血清CRP水平的变化。结果100例患者手术后1d内血清CRP水平均〉8．0mg／L；手术后3d单纯机体损伤与合并感染组，两者血清CRP水平相比较有显著性差异（P〈0．01）；手术后5d合并感染组由于应用抗生素的不同，有好转与无好转组CRP相比较也有显著性差异（P〈0．01）；手术后7d100例创伤手术患者CRP趋于正常。结论由于血清CRP的灵敏度高，在创伤患者合并感染时，有助于临床早期的鉴别诊断；同时动态检测CRP水平还有助于指导抗生素的使用及疗效观察，从而降低由于抗生素的应用而引起细菌变异及耐药性的产生。     

复方左炔诺孕酮片在健康人体的生物等效性研究

目的:评价两种复方左炔诺孕酮片在健康人体内的生物等效性。方法:19名健康男性受试者随机交叉给药,分别单次口服复方左炔诺孕酮片受试制剂或参比制剂1片(每片含左炔诺孕酮0.15mg和炔雌醇0.03mg),用放射免疫测定法测定左炔诺孕酮和炔雌醇的血药浓度,计算两种制剂中左炔诺孕酮和炔雌醇的药动学参数,并评价其生物等效性。结果:除了左炔诺孕酮的t1/2以外,受试制剂与参比制剂中左炔诺孕酮和炔雌醇的主要药动学参数无统计学差异(P>0.05)。受试制剂中左炔诺孕酮的相对生物利用度为(101.59±20.30)%,炔雌醇的相对生物利用度为(113.57±26.70)%。结论:两种复方左炔诺孕酮片具有生物等效性。     

奥沙利铂联合希罗达治疗晚期或复发性结直肠癌的临床观察

目的:观察奥沙利铂联合希罗达(卡培他滨)治疗晚期或复发性结直肠癌的临床疗效及不良反应。方法:32例晚期或复发性结直肠癌患者均给予奥沙利铂130mg/m2静滴(d1),希罗达1000mg/m2,每日两次餐后口服(d1～14),连续服用14d后停药7d。治疗周期为21d,至少治疗2个周期。结果:32例患者中CR0例,PR13例,SD10例,PD9例,总有效率40.6%,中位生存期为15.2个月,中位无进展生存期7.2个月,1年生存率为65.6%。主要毒副反应为周围神经异常、胃肠道毒性、手足综合征、口腔炎症、皮肤色素沉着。结论:奥沙利铂联合希罗达治疗晚期或复发性结直肠癌疗效确切,不良反应小,耐受性好。     

驱邪复正法在治疗急性早幼粒细胞白血病中的应用研究

急性早幼粒细胞白血病（APL）是一种特殊类型的急性白血病（AL），其临床特征为极易并发感染、出血及弥漫性血管内凝血（DIC），死亡率甚高。黄世林教授依其从事血液病临床及实验研究50余年的经验，认为白血病的病因病理基础为邪毒内蕴，骨髓瘀滞，提出“驱邪复正”为白血病的主要治则，采用解毒清热，益气活血药雄黄、青黛、太予参与丹参组方“复方黄黛片”，治疗APLl43例，60天CR为94．4％。现将黄世林教授经验整理报告如下。     

Spatial distribution and sources of polycyclic aromatic hydrocarbons (PAHs) in the reservoir sediments after impoundment of Manwan Dam in the middle of Lancang River,China

Polycyclic aromatic hydrocarbons (PAHs) have received increasing attentions owing to their carcinogenicity, teratogenicity and environmental toxicity. The studies on the spatial variations, sources identification and potential ecological risk assessment of PAHs in the reservoir sediments after dam construction are becoming new hotpots. Sixteen PAHs contamination levels were investigated from 15 sample sections in the sediments of Manwan Reservoir in the middle of Lancang River, China. Total concentrations of 16 PAHs ranged from 14.4 to 137.7 ng g^?1 dw with a mean concentration of 70.68 ng g^?1 dw. The areas with residential settlement at large tributaries and near dam had higher PAHs concentrations. In the sight of classification of PAHs pollution levels, the sediments of Manwan Reservoir could be considered as low to moderate PAHs polluted levels. One-way analysis of variance for spatial analysis revealed that there were no significant differences (P < 0.05) for 16 PAHs at the reservoir head, centre and tail. Moreover, no significant differences (P < 0.05) were found for most individual PAH at the mainstream and tributaries except that BaP showed significant differences (P < 0.05) in the mainstream and tributaries. According to the diagnostic ratios, the possible pollution sources of PAHs in Manwan Reservoir might be mixed, primarily including the petroleum source and coal combustion. As compared with sediment quality guidelines, the observed concentrations of PAHs in all sample sections did not exceed the effects range low (ERL) and the threshold effect level (TEL) values, suggesting that there were little harmful biological toxic effects on the aquatic organisms in Manwan Reservoir. The study provided a comprehensive overview on the PAHs contaminations on the reservoir sediments in the middle Lancang River, which may have an important significances on the international river management.     

关于GB/T 15835-1995中数字分节之我见

国家标准GB/T 15839-1995中规定科技出版物中的数字用法为三位分节法。分析了科技期刊排版和阅读时采用数字三位分节法存在的问题,并针对各项问题提出了建议。     

The influenza virus gene pool in a poultry market in South central china

We surveyed influenza activity in a live poultry market in Central China for 16 months, isolating viruses from 1% of 6360 fecal samples. We obtained multiple H3N6, H9N2, H2N9, H3N3, and H4N6 isolates and single H1N1 and H3N2 isolates. Two distinct H3 molecules were identified; other hemagglutinin subtypes were phylogenetically homogeneous. The H3N6 viruses (9 genotypes) and H9N2 viruses (4 genotypes) were genetically heterogeneous, whereas the H2N9, H3N3 and H4N6 viruses had single genotypes. Thirteen representative viruses were tested for their ability to replicate in quail and chickens. All tested viruses replicated in the respiratory tract of quail. Only nine of the viruses were shed in detectable levels in infected chickens, and four of these were detected in less than 50% of infected birds. A single H4N6 isolate caused disease and systemic spread in chickens. These findings show that quail are broadly susceptible to different subtypes of influenza A virus.