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排序方式: 共有289条查询结果,搜索用时 15 毫秒
1.
Kitching AR Katerelos M Mudge SJ Tipping PG Power DA Holdsworth SR 《Clinical and experimental immunology》2002,128(1):36-43
Conflicting reports exist regarding the effects of interleukin-10 (IL-10) on mesangial cells. There have been reports of both proliferative and antiproliferative effects, and both proinflammatory and anti-inflammatory effects of IL-10 on mesangial cells. However, the potential for IL-10 to affect glomerulonephritis characterized by mesangial proliferation is not known. To test the hypothesis that IL-10 would limit experimental mesangial proliferative glomerulonephritis, IL-10 was administered to rats in which mesangial proliferative glomerulonephritis was induced by administration of anti-Thy 1 antibody. Compared to control treated rats, IL-10 treated rats showed less proliferation, with fewer cells in glomeruli. Glomerular cellular proliferation was reduced, assessed by the numbers of cells within glomeruli expressing either proliferating cell nuclear antigen (PCNA) or bromodeoxyuridine. Glomerular macrophage influx (but not the proportion of glomerular macrophages that were PCNA positive) was reduced by IL-10 administration. There was no significant reduction in glomerular alpha-smooth muscle actin staining. IL-10 treatment resulted in reduced renal IL-1beta mRNA expression and reduced glomerular ICAM-1 expression, but renal expression of MCP-1 and osteopontin mRNA was unaltered. This study demonstrates that in experimental mesangial proliferative glomerulonephritis IL-10 diminishes inflammatory cell recruitment and mesangial cell proliferation. The effects of IL-10 in inhibiting mesangial cell proliferation are likely to be due to a combination of direct effects of IL-10 on mesangial cells and effects mediated by macrophages. 相似文献
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Roberts Natasha A. Janda Monika Stover Angela M. Alexander Kimberly E. Wyld David Mudge Alison 《Quality of life research》2021,30(11):3063-3071
Quality of Life Research - We evaluated the utility of the implementation science framework “Integrated Promoting Action on Research Implementation in Health Services” (i-PARIHS) for... 相似文献
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Fedor Kryuchkov Alison Robertson Elizabeth M. Mudge Christopher O. Miles Soetkien Van Gothem Silvio Uhlig 《Toxins》2022,14(6)
Ciguatera poisoning is a global health concern caused by the consumption of seafood containing ciguatoxins (CTXs). Detection of CTXs poses significant analytical challenges due to their low abundance even in highly toxic fish, the diverse and in-part unclarified structures of many CTX congeners, and the lack of reference standards. Selective detection of CTXs requires methods such as liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) or high-resolution MS (LC–HRMS). While HRMS data can provide greatly improved resolution, it is typically less sensitive than targeted LC–MS/MS and does not reliably comply with the FDA guidance level of 0.1 µg/kg CTXs in fish tissue that was established for Caribbean CTX-1 (C-CTX-1). In this study, we provide a new chemical derivatization approach employing a fast and simple one-pot derivatization with Girard’s reagent T (GRT) that tags the C-56-ketone intermediate of the two equilibrating C-56 epimers of C-CTX-1 with a quaternary ammonium moiety. This derivatization improved the LC–MS/MS and LC–HRMS responses to C-CTX-1 by approximately 40- and 17-fold on average, respectively. These improvements in sensitivity to the GRT-derivative of C-CTX-1 are attributable to: the improved ionization efficiency caused by insertion of a quaternary ammonium ion; the absence of adduct-ions and water-loss peaks for the GRT derivative in the mass spectrometer, and; the prevention of on-column epimerization (at C-56 of C-CTX-1) by GRT derivatization, leading to much better chromatographic peak shapes. This C-CTX-1–GRT derivatization strategy mitigates many of the shortcomings of current LC–MS analyses for C-CTX-1 by improving instrument sensitivity, while at the same time adding selectivity due to the reactivity of GRT with ketones and aldehydes. 相似文献
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Estimating genome conservation between crop and model legume species 总被引:31,自引:0,他引:31
Choi HK Mun JH Kim DJ Zhu H Baek JM Mudge J Roe B Ellis N Doyle J Kiss GB Young ND Cook DR 《Proceedings of the National Academy of Sciences of the United States of America》2004,101(43):15289-15294
Legumes are simultaneously one of the largest families of crop plants and a cornerstone in the biological nitrogen cycle. We combined molecular and phylogenetic analyses to evaluate genome conservation both within and between the two major clades of crop legumes. Genetic mapping of orthologous genes identifies broad conservation of genome macrostructure, especially within the galegoid legumes, while also highlighting inferred chromosomal rearrangements that may underlie the variation in chromosome number between these species. As a complement to comparative genetic mapping, we compared sequenced regions of the model legume Medicago truncatula with those of the diploid Lotus japonicus and the polyploid Glycine max. High conservation was observed between the genomes of M. truncatula and L. japonicus, whereas lower levels of conservation were evident between M. truncatula and G. max. In all cases, conserved genome microstructure was punctuated by significant structural divergence, including frequent insertion/deletion of individual genes or groups of genes and lineage-specific expansion/contraction of gene families. These results suggest that comparative mapping may have considerable utility for basic and applied research in the legumes, although its predictive value is likely to be tempered by phylogenetic distance and genome duplication. 相似文献
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Jacqueline Soraru Nicole Isbel Germaine Wong Patrick Toby Coates Murty Mantha Abu Abraham Rajiv Juneja Danny Hsu Fiona Brown Bhadran Bose David Mudge Robert Carroll Joshua Kausman Peter Hughes Thomas Barbour Anne Durkan Peter Mount Darren Lee Nicholas Larkins Dwarakanathan Ranganathan Wai H. Lim 《Nephrology (Carlton, Vic.)》2020,25(9):683-690
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Reducing systematic bias in studies of general practitioners: the use of a medical peer in the recruitment of general practitioners in research 总被引:2,自引:2,他引:0
Reducing systematic bias in any group of study participantsshould be a priority of any researcher. This can be achievedby ensuring the sampling framework is adequate and by increasingresponse rates. Response rates in studies of general practitionershave to date tended to be low. Generalization of results tothe wider population of GPs is therefore reduced. This papersystematically examines those factors which can reduce bias,recognising accurate identification of the target population,gaining good access to respondents, and maximising responserates as crucial factors. The importance of a medical peer inrecruitment is examined. Applying these factors to a study situation,three different recruitment strategies were tested. As the strategyimproved, there was an incremental improvement in the responserate (44%, 67%, 78%). These results indicate that by specificallyaddressing strategies which facilitate access to the targetpopulation, and increase the legitimacy and credibility of thestudy, significant improvements in response rates can be achieved. 相似文献
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