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排序方式: 共有280条查询结果,搜索用时 15 毫秒
1.
T D Mastro 《Annals of internal medicine》1987,106(1):173-174
2.
G Lunardi M O Vannozzi C Bighin L Del Mastro I Stevani G Schettini M Venturini 《Annals of oncology》2003,14(8):1222-1226
BACKGROUND: Anthracycline cardiotoxicity is increased by the contemporaneous administration of trastuzumab. The mechanism by which it occurs is as yet unknown. The aim of this study was to evaluate whether trastuzumab modifies the pharmacokinetics of epirubicin and its metabolites. PATIENTS AND METHODS: Women with HER2-positive metastatic breast cancer were treated with epirubicin 75 mg/m(2) i.v. bolus followed by docetaxel 75 mg/m(2) in a 1-h infusion, every 3 weeks for six cycles, and trastuzumab (once at 4 mg/m(2), then 2 mg/m(2) weekly thereafter) in a 30-min infusion. Epirubicin pharmacokinetic data of seven patients were evaluated at the first cycle of therapy (baseline, with trastuzumab administered 24 h after epirubicin), and at the sixth cycle (i.e. 15 weeks after baseline, with trastuzumab administered immediately before epirubicin). RESULTS: No pharmacokinetic change in the parent compound epirubicin was detected. The area under the plasma concentration-time curve (AUC(0-24 h)) was 1230 +/- 318 [mean +/- standard deviation (SD)] at the first cycle and 1287 +/- 385 h. micro g/l at the sixth. The mean (+/-SD) maximum plasma concentration (C(max)) and the terminal elimination half-life at the first cycle (1303 +/- 490 micro g/l and 12.5 +/- 3.1 h, respectively) were similar to those obtained at the sixth cycle (1229 +/- 580 micro g/l and 11.5 +/- 2.9 h, respectively). Pharmacokinetic data of epirubicin metabolites evaluated at the first and sixth cycle of chemotherapy were superimposable without any statistical difference. CONCLUSION: Enhanced anthracycline cardiotoxicity related to trastuzumab administration was not linked to pharmacokinetic interferences with epirubicin and its metabolites. 相似文献
3.
Koch WH Sullivan PS Roberts C Francis K Downing R Mastro TD Nkengasong J Hu D Masciotra S Schable C Lal RB 《Journal of clinical microbiology》2001,39(3):1017-1020
Six Food and Drug Administration (FDA)-licensed human immunodeficiency virus type 1 (HIV-1) and HIV-1/2 immunoassays, including five enzyme immunoassays and one rapid test, were challenged with up to 250 serum samples collected from various global sites. The serum samples were from individuals known to be infected with variants of HIV-1 including group M subtypes A, B, B', C, D, E, F, and G and group O. All immunoassays detected the vast majority of samples tested. Three samples produced low signal over cutoff values in one or more tests: a clade B sample, an untypeable sample with a low antibody titer, and a group O sample. It is concluded that HIV-1 immunoassays used in the United States are capable of detecting most HIV-1 group M variants. 相似文献
4.
Immune function did not decline with aging in apparently healthy, well-nourished women 总被引:1,自引:0,他引:1
Krause D Mastro AM Handte G Smiciklas-Wright H Miles MP Ahluwalia N 《Mechanisms of ageing and development》1999,112(1):43-57
Nutrition plays a crucial role in immune function. Most studies on age-associated changes in immunocompetence in healthy adults did not examine the nutritional status of participants extensively. Inadequate nutritional status may confound the relationship of aging and immune response. The purpose of this study was to examine age-related changes in parameters of acquired and innate immunity in healthy and generally well-nourished older (62-88 years) versus younger (20-40 years) women. Subjects were screened for participation using the health criteria of the SENIEUR protocol as well as a number of nutrition criteria related to undernutrition, and protein, iron, vitamin B12, and folate status. Young and old women did not differ in total T (CD3+), T-helper (CD4+), or T-cytotoxic (CD8+) cell number. However, older women tended to have lower T-cell proliferation response to concanavalin A (P < 0.10) and significantly reduced response to phytohemagglutinin (P < 0.05). No age-related changes were noted in natural killer cell number or cytotoxicity. Phagocytosis and subsequent oxidative burst activity also did not differ between young and old women. Most immune parameters were not compromised with aging in this cohort of apparently healthy, well-nourished women. These findings highlight the importance of simultaneous examination of health and nutritional status in studies of immune function with aging. 相似文献
5.
Lymphocyte proliferation in response to acute heavy resistance exercise in women: influence of muscle strength and total work 总被引:3,自引:0,他引:3
Dohi K Mastro AM Miles MP Bush JA Grove DS Leach SK Volek JS Nindl BC Marx JO Gotshalk LA Putukian M Sebastianelli WJ Kraemer WJ 《European journal of applied physiology》2001,85(3-4):367-373
Little is understood about the immune responses to heavy resistance exercise. The purpose of this investigation was to determine
the influence of physical strength and the ability to do more total work on lymphocyte proliferation after an acute bout of
heavy resistance exercise. A group of 50 healthy but non-strength trained women were recruited for the study and tested for
their one repetition maximum (i.e. 1 RM or maximal mass lifted once). From the normal distribution of strength the top and
bottom 8 women [mean age 22.5 (SD 3.1) years] were asked to volunteer to define our two groups (i.e. high strength and low
strength). The two groups were significantly different (P<0.05) in 1 RM squat strength [low strength 39.9 (SD 4.6) kg, 0.65 (SD 0.08) kg·kg body mass–1 and high strength 72.2 (SD 10.7) kg, 1.1 (SD 0.12) kg·kg body mass–1] but were not significantly different in body mass, age, activity levels, and menstrual status (all in same phase). Each
performed a resistance exercise protocol consisting of six sets of 10 RM squats with 2 min rest between the sets. The 10 RM
loads and total work were significantly greater in the high strength group than in the low strength group. Blood samples were
obtained pre-exercise and immediately post-exercise for test for lactate (significant increase with exercise) and cortisol
(no changes) concentrations with no differences noted between groups. Immunological assays on the blood samples determined
the incorporation of tritiated thymidine by lymphocytes in responses to concanavalin A (ConA), phytohemagglutinin (PHA), and
pokeweed mitogen (PWM). Following the squat exercise, there was a significant decrease in lymphocyte responsiveness to PWM
in the high strength but not in the low strength group for both total proliferation and proliferation adjusted per B or T
cell. On the other hand, lymphocytes from the low strength group proliferated to a significantly greater extent (adjusted
per T cell) in response to ConA and PHA. These data indicate that the heavy resistance exercise protocol reduced the lymphocyte
proliferative responses only in the stronger group of subjects. This effect may have been due to the high absolute total work
and the greater exercise stress created by the resistance exercise protocol in the high strength group. Therefore, individuals
performing at the same relative exercise intensity (i.e. 10 RM) in a resistance exercise protocol may have different immune
responses stemming from differences in absolute total work performance.
Electronic Publication 相似文献
6.
The skeleton as a unique environment for breast cancer cells 总被引:5,自引:0,他引:5
Bone is a favored location for several cancer metastases especially breast, prostate and myeloma. This review evaluates various
properties of the skeleton that contribute to its successful colonization by breast cancer cells. The first consideration
is the unique aspects of the vasculature of metaphyseal bone, which may account for the initial lodging of breast cancer cells
in specific regions of the skeleton. Metasphyseal bone, found at the ends of long bone, in ribs and in vertebrae, is comprised
of trabecular bone interspersed with marrow and a rich vasculature. The chemotactic factors that arise from bone marrow and
bone cells are discussed in terms of cancer cell migration out of the vasculature and entry of cancer cells into the marrow
cavity. Once the breast cancer cells have migrated into the metaphysis, they interact both directly and indirectly with bone
cells and other cells in the marrow. As tumor growth progresses, functional bone cells are lost, most likely through apoptosis.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
7.
8.
L Del Mastro F Perrone L Repetto L Manzione V Zagonel L Fratino D Marenco M Venturini E Maggi C Bighin T Catzeddu A Venturino R Rosso 《Annals of oncology》2005,16(2):253-258
BACKGROUND: First-line chemotherapy regimens suitable for elderly advanced breast cancer patients are still not defined. PATIENTS AND METHODS: Women with stage III or IV breast cancer aged > or =70 years were enrolled in a phase II study aimed to evaluate both activity and toxicity of weekly paclitaxel. Among 46 planned patients, at least 18 responses and not more than seven unacceptable toxic events are required for a favourable conclusion. Paclitaxel 80 mg/m(2) was administered weekly for 3 weeks every 28 days. RESULTS: Unacceptable toxicity occurred in seven out of 46 patients evaluated for toxicity [15.2%; exact 95% confidence interval (CI) 7.6% to 28.2%] and was represented by one case of febrile neutropenia, one case of severe allergic reaction and five cases of cardiac toxicity. Among 41 patients evaluated for response, a complete response occurred in two (4.9%) patients and a partial response in 20 (48.8%), with an overall response rate of 53.7% (exact 95% CI 38.7% to 67.9%). The median progression-free survival was 9.7 months (95% CI 8.5-18.7) and median survival was 35.8 months (95% CI 19-not defined). CONCLUSIONS: Weekly paclitaxel is highly active in elderly advanced breast cancer patients. Data on cardiovascular complications, however, indicate the need for a careful monitoring of cardiac function before and during chemotherapy. 相似文献
9.
Hu DJ Subbarao S Vanichseni S Mock PA Ramos A Nguyen L Chaowanachan T Griensven Fv Choopanya K Mastro TD Tappero JW 《AIDS (London, England)》2005,19(3):303-308
OBJECTIVES: To estimate the frequency and incidence of dual HIV-1 subtype infections, including superinfections, among recent seroconvertors from a cohort of injection drug users (IDUs). METHODS: A total of 1209 HIV-negative IDUs were followed in a prospective cohort study at 15 methadone clinics in Bangkok, Thailand. After 2308 person-years (PY) of follow-up, 133 seroconverted to HIV-1, of which approximately 20% were subtype B and 80% were CRF01_AE (formerly called subtype E). Specimens from 126 individuals were available at time of first seropositive test and specimens from 80 of these 126 individuals were also available more than 12 months later. For each infected participant, we calculated the amount of time to superinfection, loss to follow-up, or to the closest visit more than 12 months after the time of initial seropositivity. RESULTS: Of all 126 seroconverters seen at the time of the first seropositive test result, there was no apparent case of concurrent dual subtype infection detected despite 2301 PY of observation. Overall, the incidence of superinfection was 2.2 per 100 PY [95% confidence interval (CI), 0.3-7.8]. The 1-year incidence of CRF01_AE superinfection following subtype B primary infection was 3.9 per 100 PY (95% CI, 0.1-21.9) and the incidence of subtype B superinfection following CRF01_AE primary infection was 1.5 per 100 PY (95% CI, 0.04-8.3). CONCLUSIONS: Determination of the frequency and incidence of dual HIV-1 subtype infection demonstrates that HIV-1 superinfection is not uncommon in a population with high HIV-1 incidence with more than one circulating strain. 相似文献
10.
Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, and CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (alpha2, alpha3, alpha4, alpha5, alphav, beta1, and beta3) integrins, vinculin, as well as secretion of osteopontin (OP) and type I collagen (Col I) supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events-contact, attachment, spreading, and proliferation-are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum-compatibility-superposition principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cell-substratum-compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause and effect relationships between cell-substratum-compatibility and substratum properties. IMPACT STATEMENT: This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell line hFOB 1.19, revealing that genomic/proteomic tools are most useful in the pursuit of understanding cell adhesion if applied early in the adhesion/spreading process. 相似文献