Lens alpha-neoproteins

The formation of lens alpha-neoprotein molecules which are built by the association of A and B subunit chains of alpha-crystallin but have a different quaternary structure than the native protein is a posttranslational process that progresses through the life span. Alpha-neoproteins occur regularly in all mature mammalian lenses tested. The mechanisms that have been identified for the formation of alpha neoproteins are imbalance in the biosynthesis of A- to B-chains, which leads to a ratio of less than 2A-chains to 1B-chain, and derivation of the chains after their initial association into native alpha-crystallin. In terms of quantity of neoproteins, no significant differences have been found between lenses with developing cataracts and normal control lenses of corresponding age. The possibility that there are structural differences between alpha-neoproteins in normal and cataractous lenses of the same age, as well as potential differences among alpha-neoproteins isolated from normal lenses of different ages, are presently under investigation.     

荧光原位杂交技术分析人结肠菌群方法研究

建立荧光原位杂交技术分析人体内结肠菌群的方法。取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。结果建立最佳实验条件为 :粪便样本收集后应尽快在 4℃下保存 ,放置时间不要超过 12小时即作处理 ;样本的适宜离心条件为 70 0g 2分钟 ;样本用多聚甲醛固定后在 - 80℃下存放时间不要超过 5个月。该方法具有较好的稳定性 ,可以有效地检出个体之间结肠菌群的差异。