全文获取类型
收费全文 | 1460篇 |
免费 | 100篇 |
国内免费 | 16篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 61篇 |
妇产科学 | 10篇 |
基础医学 | 148篇 |
口腔科学 | 55篇 |
临床医学 | 132篇 |
内科学 | 291篇 |
皮肤病学 | 132篇 |
神经病学 | 81篇 |
特种医学 | 114篇 |
外科学 | 146篇 |
综合类 | 29篇 |
预防医学 | 86篇 |
眼科学 | 54篇 |
药学 | 70篇 |
中国医学 | 1篇 |
肿瘤学 | 160篇 |
出版年
2023年 | 13篇 |
2022年 | 13篇 |
2021年 | 49篇 |
2020年 | 27篇 |
2019年 | 39篇 |
2018年 | 48篇 |
2017年 | 38篇 |
2016年 | 34篇 |
2015年 | 23篇 |
2014年 | 43篇 |
2013年 | 58篇 |
2012年 | 71篇 |
2011年 | 82篇 |
2010年 | 49篇 |
2009年 | 34篇 |
2008年 | 57篇 |
2007年 | 79篇 |
2006年 | 69篇 |
2005年 | 79篇 |
2004年 | 59篇 |
2003年 | 66篇 |
2002年 | 54篇 |
2001年 | 49篇 |
2000年 | 43篇 |
1999年 | 39篇 |
1998年 | 32篇 |
1997年 | 24篇 |
1996年 | 30篇 |
1995年 | 28篇 |
1994年 | 22篇 |
1993年 | 12篇 |
1992年 | 23篇 |
1991年 | 18篇 |
1990年 | 15篇 |
1989年 | 23篇 |
1988年 | 23篇 |
1987年 | 19篇 |
1986年 | 14篇 |
1985年 | 16篇 |
1983年 | 10篇 |
1982年 | 6篇 |
1981年 | 3篇 |
1980年 | 6篇 |
1979年 | 9篇 |
1978年 | 3篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1972年 | 3篇 |
1971年 | 3篇 |
排序方式: 共有1576条查询结果,搜索用时 15 毫秒
1.
M. Masson Regnault J. Castañeda-Sanabria M.H.T. Diep Tran M. Beylot-Barry H. Bachelez N. Beneton O. Chosidow A. Dupuy P. Joly D. Jullien E. Mahé M.-A. Richard M. Viguier F. Tubach E. Sbidian C. Paul The PsoBioTeq Study Group 《Journal of the European Academy of Dermatology and Venereology》2020,34(2):293-300
2.
3.
4.
5.
Biodegradation of polydioxanone in bone tissue: effect on the epiphyseal plate in immature rabbits. 总被引:1,自引:0,他引:1
Implants of polydioxanone (PDS), 1.3 mm in diameter, were operatively fixed in the juxta-epiphyseal area of the right proximal tibial metaphysis in six rabbits and were driven into a drill hole of equal bore through the right proximal tibial epiphyseal plate in ten rabbits. The PDS implants had biodegraded almost completely in cortical bone at 16 weeks without any significant sign of inflammation or foreign body reaction. The PDS implants did not cause any growth disturbance. Histologic studies, however, showed that seven of 10 rabbits demonstrated significant osseous bridge formation across the epiphyseal plate. 相似文献
6.
A case is reported in which entrapped polymethylmethacrylate following traumatic dislocation of a total hip replacement prevented complete reduction. A combined arthroscopic and fluoroscopic technique was used to remove the entrapped polymethylmethacrylate. Manipulation of the total hip prosthesis was done after removal of polymethylmethacrylate to minimize mechanical abrasion. This technique allows direct visual assessment of the articulating surfaces as well as the mechanical stability of the prosthesis. The morbidity related to the procedure is minimal and a short rehabilitation period is the major advantage. 相似文献
7.
8.
Human ovarian granulosa cells and follicular fluid indices: the relationship to oocyte maturity and fertilization in vitro 总被引:1,自引:0,他引:1
The study investigates the correlation between oocyte maturity and
fertilization and a variety of hormonal parameters in follicular fluid and
ovarian granulosa cells. A methodology for purification of granulosa cells
from contaminating blood cells is also established. A total of 63
follicular aspirates were collected at oocyte retrieval from 30 women
superovulated using the long luteinizing hormone- releasing hormone (LHRH
analogue)/human menopausal gonadotrophin regimen. Oestradiol, progesterone,
testosterone and human chorionic gonadotrophin (HCG) were quantified in
follicular fluid and granulosa cells were immunostained for human chorionic
gonadotrophin. Immunopurification of granulosa cells from contaminating
blood cells was performed. HCG in follicular fluid was significantly high
in follicles yielding immature (grade 3) oocytes (P=0.002); there was no
correlation with fertilization. Aspirates from follicles containing mature
(grade 1) oocytes and oocytes that subsequently fertilized had
significantly more granulosa cells immunobound to HCG (P < 0.001,
P=0.02). Moreover, the immunomagnetic purification technique provided
>98% pure population of granulosa cells. The data demonstrate that HCG
in follicular fluid and on granulosa cells may help to predict oocyte
maturity and fertilization. Furthermore, immunomagnetic beads provide a
reliable procedure for the purification of ovarian granulosa cells.
相似文献
9.
Summary Induction of microtubulin paracrystals (Pc) by Kunjin (KUN) virus occurred after 15 hours post-infection and were often associated with convoluted membranes (cm) and virus particles. Vinblastine sulphate which disrupts microtubulin, had an inhibitory effect on the virus production when added during the viral latent period. When infected samples were extracted with Triton X-100 and analysed by SDS-PAGE, four viral proteins were observed. 相似文献
10.
Burwinkel B; Maichele AJ; Aagenaes O; Bakker HD; Lerner A; Shin YS; Strachan JA; Kilimann MW 《Human molecular genetics》1997,6(7):1109-1115
Glycogen storage disease due to phosphorylase kinase deficiency occurs in
several variants that differ in mode of inheritance and tissue-
specificity. This heterogeneity is suspected to be largely due to mutations
affecting different subunits and isoforms of phosphorylase kinase. The gene
of the ubiquitously expressed beta subunit, PHKB, was a candidate for
involvement in autosomally transmitted phosphorylase kinase deficiency of
liver and muscle. To identify such mutations, the complete PHKB coding
sequence was amplified by RT-PCR of RNA isolated from blood samples of
patients and analyzed by direct sequencing of PCR products. The
characterization of mutations was complemented by PCR of genomic DNA. In
one female and four male patients, we identified five independent nonsense
mutations (Y418ter; R428ter; Y974H+E975ter; Q656ter in two cases), one
single-base insertion in codon N421, one splice-site mutation affecting
exon 31, and a large deletion involving the loss of exon 8. Although these
severe translation-disrupting mutations occur in constitutively expressed
sequences of the only known beta subunit gene of phosphorylase kinase,
PHKB, they are associated with a surprisingly mild clinical phenotype,
affecting virtually only the liver, and relatively high residual enzyme
activity of approximately 10%.
相似文献