全文获取类型
收费全文 | 115篇 |
免费 | 8篇 |
专业分类
儿科学 | 4篇 |
基础医学 | 22篇 |
口腔科学 | 2篇 |
临床医学 | 15篇 |
内科学 | 19篇 |
皮肤病学 | 3篇 |
神经病学 | 6篇 |
特种医学 | 4篇 |
外科学 | 9篇 |
综合类 | 1篇 |
预防医学 | 9篇 |
眼科学 | 7篇 |
药学 | 13篇 |
肿瘤学 | 9篇 |
出版年
2023年 | 2篇 |
2022年 | 10篇 |
2021年 | 8篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 7篇 |
2017年 | 5篇 |
2016年 | 4篇 |
2015年 | 1篇 |
2014年 | 10篇 |
2013年 | 9篇 |
2012年 | 7篇 |
2011年 | 10篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2008年 | 5篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1977年 | 4篇 |
1974年 | 1篇 |
排序方式: 共有123条查询结果,搜索用时 109 毫秒
1.
L. Drgona A. Khachatryan J. Stephens C. Charbonneau M. Kantecki S. Haider R. Barnes 《European journal of clinical microbiology & infectious diseases》2014,33(1):7-21
Invasive fungal diseases (IFDs) have been widely studied in recent years, largely because of the increasing population at risk. Aspergillus and Candida species remain the most common causes of IFDs, but other fungi are emerging. The early and accurate diagnosis of IFD is critical to outcome and the optimisation of treatment. Rapid diagnostic methods and new antifungal therapies have advanced disease management in recent years. Strategies for the prevention and treatment of IFDs include prophylaxis, and empirical and pre-emptive therapy. Here, we review the available primary literature on the clinical and economic burden of IFDs in Europe from 2000 to early 2011, with a focus on the value and outcomes of different approaches. 相似文献
2.
Dominant-negative effect of the heterozygous C104R TACI mutation in common variable immunodeficiency (CVID) 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《The Journal of clinical investigation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Garibyan L Lobito AA Siegel RM Call ME Wucherpfennig KW Geha RS 《The Journal of clinical investigation》2007,117(6):1550-1557
B cells from patients with common variable immunodeficiency (CVID) who are heterozygous for transmembrane activator and CAML interactor (TACI) mutation C104R, which abolishes ligand binding, fail to produce Igs in response to TACI ligand. It is not known whether this is due to haploinsufficiency or dominant interference. Using in vitro transfection assays, here we demonstrate that C104R and the corresponding murine TACI mutant, C76R, which also does not bind ligand, dominantly interfere with TACI signaling. This effect was dependent on preassociation of the mutants with WT TACI in the absence of ligand. The mutants did not interfere with ligand binding by WT TACI, suggesting that they may act by disrupting ligand-induced receptor rearrangement and signaling. This work demonstrates that TACI preassembles as an oligomeric complex prior to ligand binding and provides a mechanistic insight into how the heterozygous C104R TACI mutation can potentially lead to CVID. 相似文献
3.
Karen Khachatryan Lusine Khachatryan Marcel Krzan Magdalena Krystyjan Lidia Krzemiska-Fiedorowicz Anna Lenart-Boro Aneta Koronowicz Mariola Drozdowska Gohar Khachatryan 《Materials》2021,14(12)
Natural polysaccharides, including hyaluronic acid, find a wide range of applications in biomedical sciences. There is a growing interest in nanocomposites containing hyaluronic acid and nanoparticles such as nanometals or graphene. In this study, we prepared foils of pure sodium hyaluronate and sodium hyaluronate containing nanosilver, graphene oxide, nanosilver/graphene oxide and characterized their properties. UV-vis spectroscopy and scanning electron microscopy (SEM) confirmed the formation of 10–20 nm silver nanoparticles. The structural changes were investigated using Fourier transforms infrared (FTIR) spectra and size exclusion chromatography. The obtained results suggest changes in molecular weights in the samples containing nanoparticles, which was highest in a sample containing nanosilver/graphene oxide. We also assessed the mechanical properties of the foils (thickness, tensile strength and elongation at break) and their wettability. The foils containing nanosilver and nanosilver/graphene oxide presented bacteriostatic activity against E. coli, Staphylococcus spp. and Bacillus spp., which was not observed in the control and sample containing graphene oxide. The composites containing graphene oxide and nanosilver/graphene oxide exhibited a cytotoxic effect on human melanoma WM266-4 cell lines (ATCC, Manassas, VA, USA). 相似文献
4.
5.
Yoshie Yoshikawa Yusuke Yamada Mitsuru Emi Lilit Atanesyan Jan Smout Karel de Groot Suvi Savola Yukako NakanishiShinkai Akihiro Kanematsu Michio Nojima Masaki Ohmuraya Tomoko HashimotoTamaoki Shingo Yamamoto 《Cancer science》2022,113(1):297
Precise quantification of copy‐number alterations (CNAs) in a tumor genome is difficult. We have applied a comprehensive copy‐number analysis method, digital multiplex ligation‐dependent probe amplification (digitalMLPA), for targeted gene copy‐number analysis in clear cell renal cell carcinoma (ccRCC). Copy‐number status of all chromosomal arms and 11 genes was determined in 60 ccRCC samples. Chromosome 3p loss and 5q gain, known as early changes in ccRCC development, as well as losses at 9p and 14q were detected in 56/60 (93.3%), 31/60 (51.7%), 11/60 (18.3%), and 33/60 (55%), respectively. Through gene expression analysis, a significant positive correlation was detected in terms of 14q loss determined using digitalMLPA and downregulation of mRNA expression ratios with HIF1A and L2HGDH (P = .0253 and .0117, respectively). Patients with early metastasis (<1 y) (n = 18) showed CNAs in 6 arms (in median), whereas metastasis‐free patients (n = 34) showed those in significantly less arms (3 arms in median) (P = .0289). In particular, biallelic deletion of CDKN2A/2B was associated with multiple CNAs (≥7 arms) in 3 tumors. Together with sequence‐level mutations in genes VHL, PBRM1, SETD2, and BAP1, we performed multiple correspondence analysis, which identified the association of 9p loss and 4q loss with early metastasis (both P < .05). This analysis indicated the association of 4p loss and 1p loss with poor survival (both, P < .05). These findings suggest that CNAs have essential roles in aggressiveness of ccRCC. We showed that our approach of measuring CNA through digitalMLPA will facilitate the selection of patients who may develop metastasis. 相似文献
6.
Raya Saab MD Anas Obeid MD Fatiha Gachi MD Houda Boudiaf MD Lilit Sargsyan MD Khulood Al-Saad MD Tamar Javakhadze MD Azim Mehrvar MD Sawsan Sati Abbas MD Yasir Saadoon Abed Al-Agele MD Salma Al-Haddad MD Mouroge Hashim Al Ani MD Suleiman Al-Sweedan MD Amani Al Kofide MD Wasil Jastaniah MD Nisreen Khalifa MD Elie Bechara MD Malek Baassiri MD Peter Noun MD Jamila El-Houdzi MD Mohammed Khattab MD Krishna Sagar Sharma MD Yasser Wali MD Naureen Mushtaq MD Aliya Batool MD Mahwish Faizan MD Muhammad Rafie Raza MD Mohammad Najajreh MD Mohammed Awad Mohammed Abdallah MD Ghada Sousan MD Khaled M. Ghanem MD Ulker Kocak MD Tezer Kutluk MD Hacı Ahmet Demir MD Hamoud Hodeish MD Samar Muwakkit MD Asim Belgaumi MD Abdul-Hakim Al-Rawas MD Sima Jeha MD 《Cancer》2020,126(18):4235-4245
7.
8.
S. A. Khachatryan K. A. Khachatryan 《Bulletin of experimental biology and medicine》1977,84(5):1562-1564
The lipolytic activity of adipose tissue (LAAT), the concentration of nonesterified fatty acids (NEFA) in the blood and adipose tissue, and the concentrations of ketone bodies and -lipoproteins in the blood of dogs were determined after lethal exsanguination and in the post-resuscitation period. During agony activation of lipolysis was found in the adipose tissue, the levels of NEFA and -lipoproteins were lowered, and the concentration of ketone bodies in the blood was increased. At the end of the third minute of clinical death inhibition of lipolysis developed and the NEFA concentration in the adipose tissue increased. The blood levels of NEFA and -lipoproteins fell 1 h after resuscitation, whereas the level of ketone bodies rose; these changes were accompanied by some decrease in LAAT. In the late postresuscitation period (1st, 3rd, and 7th days) LAAT and the blood levels of NEFA, ketone bodies, and -lipoproteins all increased. The NEFA concentration in the adipose tissue was low in the postresuscitation period.Department of Pathological Physiology, Medical Institute, Erevan. (Presented by Academician of the Academy of Medical Sciences of the USSR V. A. Negovskii.) Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 84, No. 11, pp. 544–547, November, 1977. 相似文献
9.
10.