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1.
I?Satish?RaoEmail author Anand?C?Loya KS?Ratnakar VR?Srinivasan 《BMC clinical pathology》2005,5(1):11
Background
Lymph node infarction is known to occur in association with many non-neoplastic and neoplastic conditions however its occurrence in association with DIC is not reported hitherto in the literature. 相似文献2.
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Edna Cleide Mendes Muricy Romilda Aparecida Lemes Sidney Bombarda Lucilaine Ferrazoli Erica Chimara 《Revista do Instituto de Medicina Tropical de S?o Paulo》2014,56(5):397-401
New methodologies were developed for the identification of
Nocardia but the initial diagnosis still requires a fast
and accurate method, mainly due to the similarity to
Mycobacterium, both clinical and bacteriologically. Growth
on Löwenstein-Jensen (LJ) medium, presence of acid-fast bacilli
through Ziehl-Neelsen staining, and colony morphology can be confusing aspects
between Nocardia and Mycobacterium. This study
describes the occurrence of Nocardia spp. in a
mycobacterial-reference laboratory, observing the main difficulties in
differentiating Nocardia spp. from
Mycobacterium spp., and correlating isolates with
nocardiosis cases. Laboratory records for the period between 2008 and 2012 were
analyzed, and the isolates identified as Nocardia sp. or as
non-acid-fast filamentous bacilli were selected. Epidemiological and
bacteriological data were analyzed as well. Thirty-three isolates identified as
Nocardia sp. and 22 as non-acid-fast bacilli were selected
for this study, and represented 0.12% of isolates during the study period.
The presumptive identification was based on macroscopic and microscopic
morphology, resistance to lysozyme and restriction profiles using the
PRA-hsp65 method. Nocardia spp. can grow
on media for mycobacteria isolation (LJ and BBL MGIT™) and
microscopy and colony morphology are very similar to some mycobacteria species.
Seventeen patients (54.8%) were reported and treated for
tuberculosis, but presented signs and symptoms of nocardiosis. It was concluded
that the occurrence of Nocardia sp. during the study period was
0.12%. Isolates with characteristics of filamentous bacilli, forming aerial
hyphae, with colonies that may be pigmented, rough and without the BstEII
digestion pattern in PRA-hsp65 method are suggestive of
Nocardia spp. For a mycobacterial routine laboratory, a
flow for the presumptive identification of Nocardia is
essential, allowing the use of more accurate techniques for the correct
identification, proper treatment and better quality of life for patients. 相似文献
5.
Adriela Azevedo Souza Mariath Ana Eliza Lemes Bressani Fernando Borba de Araujo 《Journal of applied oral science : revista FOB》2007,15(6):529-533
The purpose of this study was to validate the elastomeric impression after temporary tooth separation as a method of cavitation detection in proximal caries lesions in primary molars with outer half dentin radiolucency. Fifty-one children (4-10 years old), presenting radiolucency in the outer half of the dentin at the proximal surfaces of primary molars and proximal anatomic contact with the adjacent tooth (without restoration/cavitated caries lesion) were enrolled in the study. Temporary tooth separation was performed with an orthodontic rubber ring placed around the contact point during 2-3 days. Thereafter, impression of the proximal surfaces was made. The elastomeric impressions were classified as "non-cavitated" or "cavitated" surfaces. Visual inspection after tooth separation was considered as the gold standard. Examiner reliability of visual inspection after tooth separation was determined (kappa 0.92). Impression examination was repeated every 5 participants to evaluate the reproducibility of the method. The frequency of cavitated lesions was 65%, and 67% of those were inactive. Sensitivity, specificity, positive and negative predictive values were 0.88% (95%CI 0.73-0.95), 0.89% (95%CI 0.67-0.97), 0.94% (95%CI 0.79-0.98) and 0.80% (95%CI 0.58-0.92), respectively. Impression examination showed total agreement regarding cavitation. The evaluation of elastomeric impression after tooth separation is a useful clinical resource in cavitation detection for clinicians and researchers when visual inspection is doubtful. 相似文献
6.
Arruda VR; Pieneman WC; Reitsma PH; Deutz-Terlouw PP; Annichino-Bizzacchi JM; Briet E; Costa FF 《Blood》1995,86(8):3015-3020
The molecular characterization of the mutations in hemophilia A patients is hampered by the large size of the factor VIII gene and the great heterogeneity of mutations. In this study, we have performed a protocol involving multiplex polymerase chain reaction in which 19 exons were amplified in four different combinations followed by nonradioactive single-strand conformational polymorphism (SSCP) to screen for mutations. Southern blotting was used to detect inversion of the factor VIII gene resulting from recombination between copies of the gene A (F8A) located in intron 22 of the factor VIII gene and two copies close telomeric region of X chromosome. Forty-two hemophilia A patients (21 with severe and 21 with mild-to-moderate disease) were studied. The inversion of factor VIII occurred in 13 of 21 patients affected by severe hemophilia A. One patient showed a large extra band in addition to the three bands observed after Southern blotting with the F8A probe. An abnormal electrophoretic pattern of SSCP was detected in 85% and 50% of the patients affected by mild-to-moderate and severe disease, respectively. Sixteen different mutations were identified. Eleven mutations were novel and comprised 9 point mutations and 2 small deletions. This study shows that the methodology used is safe and rapid and has potential for detecting almost all of the genetic defects of the studied hemophilia A patients. 相似文献
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Molero T Roemer B Perera Alvarez Mdel M Lemes A Iñigo Sde L Palacios G Scott CS 《Clinical and laboratory haematology》2005,27(4):224-234
This communication details a method for the quantitative and qualitative analysis of blood T-, B- and NK-cell populations using the Abbott Cell-Dyn CD4000 haematology analyser. A series of 66 ethylenediaminetetraacetic acid (EDTA)-anticoagulated samples with lymphocyte counts between 0.2 and 33.3 x 10(9)/l were selected and analysed with CD3, CD19, Ia and CD56 monoclonal reagents. The flow cytometry reference method utilized a lymphocyte gate defined by optical scatter, with phenotypic analyses referencing to this gate and the absolute lymphocyte count. The CD4000 method analysed all leucocyte events, set primary gates for specific immunophenotypic fractions, and then determined population counts by reference to the white blood cell (WBC) count. Comparisons of CD3+ T-cell and CD19+ B-cell numbers showed high coefficients of correlation (R(2) > 0.95) and agreement (y = 1.01x) between the CD4000 and flow cytometry reference methods. Lower coefficients of correlation were obtained for CD3-CD56+ (R(2) = 0.52) and CD3+CD56+ (R(2) = 0.83) components. No major discrepancies were observed, and the CD4000 procedures additionally provided qualitative insights into the possibility of T-cell activation. The potential to undertake immediate analysis of EDTA-anticoagulated blood samples to determine the nature of abnormal lymphocyte morphology or numbers represents a considerable advance in the capability of haematology laboratories. 相似文献
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