基于太赫兹时域光谱技术的名贵动物药材龟甲的真伪品鉴别

目的： 建立一种利用太赫兹时域光谱和多种化学计量学方法鉴别龟甲真伪的新方法。方法： 选用正品乌龟和伪品红耳彩龟的腹甲进行实验,先用经典的性状鉴别、显微鉴别方法比较两种样品的典型性状特征,后利用太赫兹时域光谱仪测得样品的光谱信息,对样品谱图进行平滑处理和基线校准,并运用化学计量方法进行数据分析以区分正品和伪品龟甲。结果： 性状鉴别结果显示完整正、伪品龟甲样品仅有细微差异,且其碎片或粉末基本无差异;显微鉴别未在两种样品中发现明显的特征差异性结构;两种样品的太赫兹时域光谱曲线图经快速傅里叶变换滤波器平滑滤波处理、正交偏最小二乘法判别分析处理后呈现显著差异。结论： 利用太赫兹时域光谱技术结合正交偏最小二乘法判别分析可以实现龟甲真伪品的精准鉴别。     

整体责任制护理对乳腺癌手术患者生活质量及护理满意度的影响

目的探讨整体责任制护理对乳腺癌手术患者生活质量及护理满意度的影响。方法选取2019年6月至2020年6月期间于我院行手术治疗的71例乳腺癌患者,随机分为两组。对照组35例采用常规护理,观察组36例采用整体责任制护理。比较两组护理前后的生活质量及护理满意度。结果护理后,两组的生活质量各维度评分均显著高于护理前,且观察组的生活质量评分均显著高于对照组(P <0.05)。观察组的护理满意度为91.67%,显著高于对照组的68.57%(P <0.05)。结论整体责任制护理可有效改善乳腺癌手术患者的生活质量,提升其护理满意度。     

A head-to-head comparison of measurement properties of the EQ-5D-3L and EQ-5D-5L in acute myeloid leukemia patients

Quality of Life Research - This study aimed to compare the measurement properties of EQ-5D-3L(3L) and EQ-5D-5L(5L) in patients with acute myeloid leukemia (AML) in China. We consecutively recruited...     

小柴胡汤分解剂抗柯萨奇B3病毒感染及其对心肌炎防治作用的研究

目的 :探讨小柴胡汤分解剂在体外及体内抗柯萨奇 B3病毒 (CVB3)及保护心肌细胞的作用。方法 :用小柴胡汤分解剂通过体外微量细胞培养 ,作用于被 CVB3直接损伤的细胞模型及经腹腔感染 CVB3m Balb/ c小鼠心肌炎模型 ,分析不同浓度分解剂的效应。结果 :在体外细胞上分解剂 A、B液自 1∶ 32稀释度对 vero细胞的毒性作用已丧失 ,至 1∶ 2 5 6稀释度时 ,仍能对抗 CVB3对细胞致病变作用。在体内分解剂 A、B液治疗组小鼠 35只 ,心肌炎发生率 37.14% ;而 2 0只对照组小鼠心肌炎发生率 5 9.1% ,两者有显著性差异 (χ2 =4.6 8,P <0 .0 5 ) ,在预防给药组更为明显 (P <0 .0 1)。结论 :小柴胡汤分解剂 A、B液在体内、体外均具有抗病毒及保护心肌的作用。     

阿魏酸对视网膜神经细胞内游离钙浓度变化的影响

目的研究阿魏酸(FA)对视网膜神经细胞内游离钙([Ca2 ]i)水平变化的影响。方法以胚胎7个月人视网膜,新生小牛视网膜和生后4个月小鼠视网膜为研究对象,检测FA作用后[Ca2 ]i水平变化。结果500μg/mLFA作用下,胚胎人组[Ca2 ]i荧光强度(基值为57.08±3.97)在10s内降至38.41±4.92,维持在38.41±4.92～43.45±3.29;新生小牛组[Ca2 ]i(基值为69.48±3.87)在20s内降至52.86±3.69,维持在49.75±3.82～54.39±4.04;成年小鼠组[Ca2 ]i(基值为71.54±3.73)在20s内降至54.91±3.57,维持在53.81±3.49～58.14±3.49,且发现胚胎人组[Ca2 ]i基值和受光照射后变化明显异于新生期小牛组和成年小鼠组。结论FA能有效降低视网膜神经细胞内游离钙水平,对于促进增殖和保护细胞具有重要作用。     

孕早期子宫颈管脱落滋养细胞的收集与影响因素探讨

目的:探讨孕早期子宫颈管脱落滋养细胞的收集与影响因素,为进一步的孕早期无创性产前诊断奠定基础。方法:从早孕妇女的宫颈管获取脱落的滋养细胞并探讨影响因素。结果:双取器宫颈管毛刷法获取的阳性率最高;孕5~7周是取材较适宜的时间;妊娠囊下缘至宫颈内口的距离对获取的滋养细胞影响较小。结论:孕早期获取宫颈管脱落滋养细胞是可行的;取材工具,取材孕周对获取孕早期脱落滋养细胞有一定的影响。     

The Role of Self-Beliefs in Predicting Postschool Outcomes for Deaf Young Adults

The aim of this study was to explore the role of self-beliefs in predicting postschool outcomes for deaf young adults in transition from secondary settings. Three self-level constructs were explored: self-concept, self-determination, and expectations about the future. This study utilized data from the National Longitudinal Transition Study-2 (NLTS2) that collected longitudinal data from youths with disabilities across the nation, 550 of which were deaf or hard of hearing and met the selection criteria in this study. This study examined the relationships between these deaf adolescents’ self-beliefs and actual future achievements that were reached as they transitioned to adult life, in three domains: life, employment, and education. Despite the generally positive self-beliefs of deaf individuals, which were, in some cases, related to postschool outcomes, the self-beliefs assessed in this study did not emerge as comprehensive predictors of postschool attainments. Findings suggest that for deaf individuals, successfully navigating transitions to adult life involves dimensions beyond individual agency. Positive self-beliefs are clearly a part of successfully attaining postschool outcomes, but deaf individuals may not have full access to equitable opportunities to capitalize on these beliefs.     

Buformin suppresses proliferation and invasion via AMPK/S6 pathway in cervical cancer and synergizes with paclitaxel

Buformin is an old anti-diabetic agent and manifests potent anti-tumor activities in several malignancies. In the present study, we aimed to explore the functions of buformin in human cervical cancer. As our data shown, buformin exhibited significant anti-proliferative effects in a dose-dependent manner in 4 cervical cancer cell lines. Compared to the control, buformin notably suppressed colony formation and increased ROS production in C33A, Hcc94 and SiHa cells. Flow cytometric analysis showed that buformin induced marked cell cycle arrest but only resulted in mild apoptosis. The invasion of C33A and SiHa cells sharply declined with buformin treatment. Consistently, western blotting showed that buformin activated AMPK and suppressed S6, cyclin D1, CDK4, and MMP9. Moreover, we found that buformin enhanced glucose uptake and LDH activity, increased lactate level, while decreased ATP production in cervical cancer cells. In addition, low doses of buformin synergized with routine chemotherapeutic drugs (such as paclitaxel, cisplatin, and 5-FU) to achieve more significant anti-tumor effects. In vivo, a single use of buformin exerted moderate anti-tumor effects, and the combination with buformin and paclitaxel exhibited even greater suppressive effects. Buformin also consistently showed synergistic effects with paclitaxel in treating primary cultures of cervical cancer cells. Take together, we are the first to demonstrate that buformin suppresses cellular proliferation and invasion through the AMPK/S6 signaling pathway, which arrests cell cycle and inhibits cellular invasion. Buformin also could synergize with routine chemotherapies, producing much more powerful anti-tumor effects. With these findings, we strongly support buformin as a potent choice for treating cervical cancer, especially in combination with routine chemotherapy.     

Distribution of eight blood-group systems and ABH secretion in Mongolian,Korean, and Zhuang nationalities in China

Among different blood-group systems investigated here, the distribution of the ABO system varies most of all, followed by MNSs and P. Although there are differences in ABH secretion in the different populations, no striking regularity has been found. No significant difference has been found in the distribution of the blood-group systems Rhesus, Diego, Duffy, Kell and Kidd in these Chinese populations: the Rh (-) rate is less than 1%, the frequencey of Di^a is about 0·04, the frequency of Fy^a is rather high, the frequency of K extremely low and Jk^a is about 0·4.

In the three nationalities under study, Mongolians are characterized by a high frequency of I^B, M higher than 0·5 and a relatively high frequency of P₁. Koreans are characterized by a high frequency of I^A, slightly more M than N and rather high CDE in the Rh system. The peculiarities of the Zhuangs are a rather high frequency of I^O and M and the lowest frequency of P₁.