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Anthony F. Henwood 《Journal of histotechnology》2020,43(2):102-104
ABSTRACT The 2019 Coronavirus epidemic, provisionally called 2019-nCoV, was first identified in Wuhan, China, in persons exposed to a seafood or wet market. There is an international push to contain the virus and prevent its spread. It is feasible that potentially infectious samples may be received in histopathology laboratories for diagnosis. This technical note presents disinfection procedures and histotechnology processes that should alleviate the risk of infection to laboratory staff. Using data obtained from similar coronaviruses, e.g. severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), experts are confident that 70% ethanol and 0.1% sodium hypochlorite should inactivate the virus. Formalin fixation and heating samples to 56oC, as used in routine tissue processing, were found to inactivate several coronaviruses and it is believed that 2019-nCoV would be similarly affected. 相似文献
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Lichtner P Attié-Bitach T Schuffenhauer S Henwood J Bouvagnet P Scambler PJ Meitinger T Vekemans M 《Journal of molecular medicine (Berlin, Germany)》2002,80(7):431-442
Partial monosomy 10p is a rare chromosomal aberration. Patients often show symptoms of the DiGeorge/velocardiofacial syndrome spectrum. The phenotype is the result of haploinsufficiency of at least two regions on 10p, the HDR1 region associated with hypoparathyroidism, sensorineural deafness, and renal defects (HDR syndrome) and the more proximal region DGCR2 responsible for heart defects and thymus hypoplasia/aplasia. While GATA3 was identified as the disease causing gene for HDR syndrome, no genes have been identified thus far for the symptoms associated with DGCR2 haploinsufficiency. We constructed a deletion map of partial monosomy 10p patients and narrowed the critical region DGCR2 to about 300 kb. The genomic draft sequence of this region contains only one known gene, BRUNOL3 ( NAPOR, CUGBP2, ETR3). In situ hybridization of human embryos and fetuses revealed as well as in other tissues a strong expression of BRUNOL3 in thymus during different developmental stages. BRUNOL3 appears to be an important factor for thymus development and is therefore a candidate gene for the thymus hypoplasia/aplasia seen in partial monosomy 10p patients. We did not find BRUNOL3 mutations in 92 DiGeorge syndrome-like patients without chromosomal deletions and in 8 parents with congenital heart defect children. 相似文献
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Henwood M 《The Health service journal》1994,104(5396):22-24
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Anthony Henwood 《Journal of histotechnology》2013,36(4):177-181
ABSTRACTThe histopathologic assessment of placenta is often marred by poorly fixed and processed sections. An audit conducted in the department resulted in 21% of placental blocks showing features of poor processing. A microwave-assisted fixation procedure was introduced using the Micromed KOS microwave oven (ABACUS-ALS, Brisbane, Australia) for 2 h at 45°C. This resulted in an improvement in the quality of the sections with a zero failure rate on a subsequent audit. 相似文献
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Piotr Kocur Marzena Wiernicka Maciej Wilski Ewa Kaminska Lech Furmaniuk Marta Flis Maslowska Jacek Lewandowski 《Journal of Physical Therapy Science》2015,27(12):3733-3737
[Purpose] To assess the effect of 12-weeks Nordic walking training on gait parameters and
some elements of postural control. [Subjects and Methods] Sixty-seven women aged 65 to
74 years were enrolled in this study. The subjects were divided into a Nordic Walking
group (12 weeks of Nordic walking training, 3 times a week for 75 minutes) and a control
group. In both study groups, a set of functional tests were conducted at the beginning and
at the end of the study: the Forward Reach Test (FRT) and the Upward Reach Test (URT) on a
stabilometric platform, and the analysis of gait parameters on a treadmill. [Results] The
NW group showed improvements in: the range of reach in the FRT test and the URT test in
compared to the control group. The length of the gait cycle and gait cycle frequency also
showed changes in the NW group compared to the control group. [Conclusion] A 12-week NW
training program had a positive impact on selected gait parameters and may improve the
postural control of women aged over 65 according to the results selected functional
tests.Key words: Nordic walking, Postural control, Gait 相似文献
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Nieborowska-Skorska M Kopinski PK Ray R Hoser G Ngaba D Flis S Cramer K Reddy MM Koptyra M Penserga T Glodkowska-Mrowka E Bolton E Holyoake TL Eaves CJ Cerny-Reiterer S Valent P Hochhaus A Hughes TP van der Kuip H Sattler M Wiktor-Jedrzejczak W Richardson C Dorrance A Stoklosa T Williams DA Skorski T 《Blood》2012,119(18):4253-4263
Chronic myeloid leukemia in chronic phase (CML-CP) is induced by BCR-ABL1 oncogenic tyrosine kinase. Tyrosine kinase inhibitors eliminate the bulk of CML-CP cells, but fail to eradicate leukemia stem cells (LSCs) and leukemia progenitor cells (LPCs) displaying innate and acquired resistance, respectively. These cells may accumulate genomic instability, leading to disease relapse and/or malignant progression to a fatal blast phase. In the present study, we show that Rac2 GTPase alters mitochondrial membrane potential and electron flow through the mitochondrial respiratory chain complex III (MRC-cIII), thereby generating high levels of reactive oxygen species (ROS) in CML-CP LSCs and primitive LPCs. MRC-cIII-generated ROS promote oxidative DNA damage to trigger genomic instability, resulting in an accumulation of chromosomal aberrations and tyrosine kinase inhibitor-resistant BCR-ABL1 mutants. JAK2(V617F) and FLT3(ITD)-positive polycythemia vera cells and acute myeloid leukemia cells also produce ROS via MRC-cIII. In the present study, inhibition of Rac2 by genetic deletion or a small-molecule inhibitor and down-regulation of mitochondrial ROS by disruption of MRC-cIII, expression of mitochondria-targeted catalase, or addition of ROS-scavenging mitochondria-targeted peptide aptamer reduced genomic instability. We postulate that the Rac2-MRC-cIII pathway triggers ROS-mediated genomic instability in LSCs and primitive LPCs, which could be targeted to prevent the relapse and malignant progression of CML. 相似文献
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