Improved Maternal and Infant Outcomes with Serial,Self-Reported Early Prenatal Substance Use Screening

Maternal and Child Health Journal - Most screening tools identifying women with substance use are not validated, used once in pregnancy, and are not reflective of continued substance use. We...     

ALTERNATIVE REDUCING AGENTS FOR REDUCTIVE METHYLATION OF AMINO GROUPS IN PROTEINS

Dimethylamine borane and trimethylamine borane were compared with sodium cyanoborohydride as reducing agents for the reductive methylation of amino groups in proteins. Dimethylamine borane performed nearly as well as cyanoborohydride; either reducing agent (15 mM) with 20 mM formaldehyde gave extensive methylation of turkey ovomucoid (5 mg/ml) in 2h at 22°. Trimethyl amine borane gave equivalent levels of methylation only with a higher concen tration of formaldehyde, suggesting that it is an even milder reducing agent than the other two under these conditions. With all three reducing agents, the pH optimum for methylation covered a range of pH 6–9. Methylations should be performed at the lowest possible pH to avoid side reactions of formaldehyde with the protein. Possible effects of these reducing agents on the disulfide bonds of proteins were studied. No reduction of disulfides of turkey ovomucoid was observed using each of the three reducing agents under conditions for methylation.