海南省黎族中学生艾滋病知识干预效果分析

目的：通过干预提高黎族中学生的艾滋病知识水平，降低黎族人群感染艾滋病的危险。方法：采用集中授课、播发VCD等方式进行干预，利用自行设计的调查表对干预对象进行相关知识调查。结果：干预后中学生对艾滋病相关知识的认识均有不同程序的提高，最高提高22．90个百分点，最低提高6．50个百分点，平均提高15．46个百分点；艾兹病知识主要来源于电视广播和医生，分别占58．00％和22．00％。结论：在黎族中学生中开展艾滋病健康教育是完全可行且是十分必要的，应该引起重视。     

抗CD3单克隆抗体重、轻链可变区基因的克隆及序列分析

目的：抗CD3单克隆抗体（WuT3)可变区基因克隆及序列分析。方法；采用RT-PCR技术，从WuT3杂交瘤细胞总RNA中扩增VH，VL片段，经酶切后通过链接反应构建重组克隆载体，测序鉴定。结果：通过国际联机检索发现VH，VL基因与Ig同源，分别符合小鼠IgVH,Vк基因特征。VH基因属于鼠重链VH第ⅡB亚类，全长363bp，可编码121个氨基酸；VL基因属于鼠к轻链第Ⅲ亚类，全长330bp，可编码110个氨基酸，结论：成功获得WuT3单抗的重，轻链可变区基因。     

基于GEPIA和Kaplan–Meier Plotter数据库分析蛋白酶活化受体表达对胃癌患者生存结局的影响

目的：探讨蛋白酶活化受体（PARs）家族成员在胃癌中的表达状态对患者生存结局的影响。方法：基于基因表达谱交互分析（GEPIA）数据库分析PARs成员信使核糖核酸（mRNA）在胃癌组织中的表达水平，并借助Kaplan–Meier Plotter在线工具分析PAR1～4表达对患者总生存期（OS）、进展生存期（FP）及复发后生存期（PPS）的影响。结果：GEPIA收录数据分析表明，尽管PAR1～4在胃癌组织中表达升高，但PAR4在肿瘤组织和对照样本中的表达差异无统计学意义（P> 0.05）。Kaplan–Meier Plotter分析结果表明，PAR1表达并不影响胃癌患者的临床生存期；PAR2升高会缩短患者复发后的生存期；PAR3和PAR4升高则大幅度缩减患者的临床生存期。结论：PAR3和PAR4在肿瘤中的表达状态可作为判断胃癌患者预后生存期的参考指标。     

血友病的基因治疗

长期以来 ,单基因遗传病的诊断主要靠临床观察和系列化学检查 ,但生化学检查要求有相应基因表达产物的体液或细胞 ,并对基因产物或代谢异常机理有所了解 ,但对绝大多数遗传病而言 ,目前还远未达到这种认识。各种类型的血友病 ,这些令人终生痛苦的疾病 ,都是由于凝血因子Ⅷ或凝血因子Ⅸ (表 1)的遗传上的缺乏。这些血液凝固因子的基因在X染色体上 ,当它们发生了变异的时候 ,这些突变的X引起X连锁的隐性的血友病A和B ,它们通常发生在男性中。受影响的男孩子已经继承了他的携带者母亲 (XH/X)的变异的基因 (XH) ,但有大约 30 %的情况由自发…     

Anticancer effect of icaritin on human lung cancer cells through inducing s phase cell cycle arrest and apoptosis

Icaritin, a prenylflavonoid derivative from Epimedium Genus, has been shown to exhibit many pharmacological and biological activities. However, the function and the underlying mechanisms of icaritin in human non-small cell lung cancer have not been fully elucidated. The purpose of this study was to investigate the anticancer effects of icaritin on A549 cells and explore the underlying molecular mechanism. The cell viability after icaritin treatment was tested by MTT assay. The cell cycle distribution, apoptosis and reactive oxygen species（ROS） levels were analyzed by flow cytometry. The mRNA and protein expression levels of the genes involved in proliferation and apoptosis were respectively detected by RT-PCR and Western blotting. The results demonstrated that icaritin induced cell cycle arrest at S phase, and down-regulated the expression levels of S regulatory proteins such as Cyclin A and CDK2. Icaritin also induced cell apoptosis characterized by positive Hoechst 33258 staining, accumulation of the Annexin V-positive cells, increased ROS level and alteration in Bcl-2 family proteins expression. Moreover, icaritin induced sustained phosphorylation of ERK and p38 MAPK. These findings suggested that icaritin might be a new potent inhibitor by inducing S phase arrest and apoptosis in human lung carcinoma A549 cells.     

Fucoidan induces apoptosis of HepG2 cells by down-regulating p-Stat3

Summary： Fucoidan is one of the main bioactive components of polysaccharides. The current study was focused on the anti-tumor effects of fucoidan on human heptoma cell line HepG2 and the possible mechanisms. Fucoidan treatment resulted in cell cycle arrest and apoptosis of HepG2 cells in a dose-dependent manner detected by MTT assay, flow cytometry and fluorescent microscopy. The results of flow cytometric analysis revealed that fucoidan induced G2/M arrest in the cell cycle progression. Hoechst 33258 and Annexin V/PI staining results showed that the apoptotic cell number was increased, which was associated with a dose-dependent up-regulation of Bax and down-regulation of Bcl-2 and p-Stat3. In parallel, the up-regulation of p53 and the increase in reactive oxygen species were also observed, Which may play important roles in the inhibition of HepG2 growth by fucoidan. In the meantime, Cyelin B 1 and CDK1 were down-regulated by fucoidan treatment. Down-regulation of p-Stat3 by fucoidan resulted in apoptosis and an increase in ROS in response to fucoidan exposure. We therefore concluded that fucoidan induces apoptosis through the down-regulation of p-Stat3. These results suggest that fucoidan may be used as a novel anti-cancer agent for hepatocarcinoma.     

海南岛首次从三带喙库蚊分离到两株乙型脑炎病毒

我区过去向有流行性乙型脑炎(简称乙脑)的流行,但对其病原及虫媒尚未进行过有效的监测,1983年9月在白沙县对常见蚊类进行了一次分离病毒的研究。结果从三带喙库蚊分     

海南岛轮状病毒所致之婴幼儿腹泻的抗原检测及流行病学调查研究

海南岛各县市每年秋冬季均有不少婴幼儿腹泻流行,以前曾对其细菌性病原进行过探索,但尚未做过病毒检查。1982年12月至次年2月,受世界卫生组织委托,经中央卫生部的安排,我区接受了进行轮状病毒抗原检测的任务。在海南岛部分县(市)医院门诊及病房,采集了部分临床诊断为“婴幼儿秋季腹泻”、“急性胃肠炎”、“消化不良”的小儿新鲜粪     

海南岛家鸭血清中检出HBsAg

乙型肝炎传染源多而且传播途径复杂,是一种难于控制的世界性传染病。动物与人类乙型肝炎的关系如何?根据国内外文献报导,目前与 HBV 相类似的病毒有土拔鼠病毒,美国野地鼠体内分离的 GSHBsAg、中国鸭血清中的 DHBV 等。为试图了解家鸭与人类 HBsAg 的关系,1984年8月间在海口市集市鸭群中采集鸭血     

基因治疗的进展、问题和展望

1997年4月13～19日,在美国犹他州斯诺伯德召开了基因治疗的分子和细胞生物学会议。会议报告了在基因送递、基因调控及避免免疫应答等新技术方面取得的进展。此外,会议还讨论了应用新基因治疗特定遗传病和获得性疾病的进展以及基因治疗的问题和前景。