新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

目的 探讨磁共振(magnetic resonance,MR)技术在筛查食管癌中的作用及分析不同民族食管癌血浆代谢组学差异.方法 收集新疆医科大学第一附属医院胸外科确诊的108例食管癌患者和50名健康人血液标本,应用MR技术结合正交偏最小二乘判别分析法(orthogonal partial least-squares discriminant analysis,OPLS-DA),分析新疆哈萨克族、汉族和维吾尔族食管癌患者与健康人血浆代谢物指纹图谱,确定食管癌患者及不同民族食管癌的差异性代谢组分.结果 食管癌患者和健康人血浆多种代谢组分有显著性差异.与对照组相比,食管癌患者血浆中多种代谢物显著下降,包括亮氨酸(0.0043±0.0006、0.0040±0.0006)、丙氨酸(0.0039±0.O007、0.0033±0.O006)、异亮氨酸(0.0067±0.0010、0.0063±0.O009)、缬氨酸(0.0037±0.0005、0.0035±0.0006)、糖蛋白(0.0123±0.0043、0.0102±0.0022)、乳酸(0.0342±0.0113、0.0258±0.0085)、丙酮(0.0027±0.0023、0.0017±0.0008)、乙酸(0.0007±0.0001、0.0006±0.0001)、胆碱(0.0035±0.0006、0.0029±0.0007)、异丁酸(0.0020±0.0004、0.0018±0.0003)及脂肪分解产物不饱和脂类(0.0072±0.0013、0.0059±0.0018)、极低密度脂蛋白(0.1209±0.0589、0.0879±0.0269)、低密度脂蛋白(0.0885±0.0328、0.0785±0.0288)和1-甲基组氨酸(0.0005±0.0001、0.0004±0.0005)等,差异有统计学意义(r值均>0.27,P值均<0.05).食管癌患者血浆中二甲胺(0.0004±0.0001、0.0005±0.0001)、α-葡萄糖(0.0079±0.0013、0.0081±0.0016)和β-葡萄糖(0.0139±0.0024、0.0142±0.0029)、柠檬酸(0.0044±0.0008、0.0106±0.0058)等明显增加,与健康人比较差异有统计学意义(r值均<-0.27,P值均<0.05).汉族与哈萨克族食管癌患者比较,血浆中丙氨酸(0.0031±0.0005、0.0029±0.0004)、谷氨酰胺(0.0010±0.0001、0.0009±0.0001)、酪氨酸(0.0009±0.0001、0.0008±0.0001)、1-甲基组氨酸(0.0005±0.0001、0.0004±0.0001)含量显著增加(r值均>0.35,P值均<0.05),哈萨克族患者血浆中肉碱(0.0028±0.0006)含量明显高于汉族患者(0.0025±0.0004)(r=-0.40,P<0.05);维吾尔族与哈萨克族患者比较,血浆中不饱和脂类(0.0059±0.0018、0.0047±0.0011)、异亮氨酸(0.0062±0.0011、0.0058±0.0007)、丙氨酸(0.0032±0.0007、0.0028±0.0004)、糖蛋白(0.0096±0.0019、0.0086±0.0011)、谷氨酰胺(0.0011±0.0001、0.0009±0.0001)、酪氨酸(0.0009±0.0001、0.0008±0.0001)、1.甲基组氨酸(0.0005±0.0001、0.0004±0.0001)含量显著增加(r值均>0.33,P值均<0.05),哈萨克族患者血浆中肉碱(0.0027±0.0005)含量显著高于维吾尔族患者(0.0025±0.0004)(r=-0.36,P<0.05).结论 以代谢组学为基础的MR技术是无创、快速、高度敏感的检测方法 ,在食管癌早期诊断和高危人群筛查方面具有独特优势和应用潜力.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.