大豆异黄酮对局灶性脑缺血再灌注损伤大鼠血脑屏障通透性的影响及其机制

目的 探讨大豆异黄酮(SI)对局灶性脑缺血再灌注大鼠的血脑屏障(BBB)通透性的影响及相关机制。方法 72只健康成年SD大鼠均分为假手术组(Sham组)、缺血再灌注组(I/R组)和SI预处理组(SI组),每组各24只。SI组每天按120 mg/kg体质量给予12 mg/mL SI灌胃1次,Sham组和I/R组每天用等体积生理盐水灌胃1次,均连续灌胃21 d。在实验第22天,I/R组和SI组大鼠用线栓阻断右侧大脑中动脉血流制作大脑中动脉缺血再灌注动物模型;Sham组不插入线栓,未阻断血流,其余手术操作与I/R和SI组一致。I/R组和SI组大鼠缺血2 h后,拔出阻塞的线栓恢复血流,再灌注24 h。根据Zea longa评分,选择造模成功的大鼠。采用2,3,5-氯化三苯基四氮唑(TTC)检测大鼠脑梗死体积,干湿重法检测脑组织含水量,伊文思蓝(EB)示踪法检测BBB通透性,Western blot检测脑组织MMP-9、TIMP-1蛋白表达。比较三组大鼠的测量数据,分析SI预处理对以上观测指标的影响。结果 SI组和I/R组大鼠的神经功能评分、脑梗死体积、脑组织含水量和脑组织内EB含量均高于Sham组,而SI组低于I/R组,分别为(1.50±1.13)、(2.33±0.82)和(0.00±0.00)分,12.5%±2.34%、37.50%±2.28%和0.07%±0.03%,81.75%±0.86%、84.17%±0.54%和78.24%±0.41%,(46.50±1.41)、(55.44±1.37)和(3.83±1.49)μg/g,差异均有统计学意义(P值均＜0.01)。SI组和I/R组MMP-9、TIMP-1表达均明显高于Sham组,分别为0.81±0.23、1.15±0.14、1.15±0.14和2.31±0.20、1.62±0.21、1.62±0.21,差异均有统计学意义(P值均<0.01);与I/R组相比,SI组MMP-9表达减少、TIMP-1表达增加,差异均有统计学意义(P值均＜0.01)。结论 SI可能通过下调缺血再灌注后脑组织MMP-9蛋白表达和上调TIMP-1蛋白表达,降低BBB的通透性,减轻缺血再灌注后脑水肿的程度和脑组织EB的含量。     

Effects of different moxibustion times on TRPV3 ion channel protein and synovial cell apoptosis in rats with rheumatoid arthritis

Objective

To observe the effects of different moxibustion times on proteins of transient receptor potential vanilloid 3 (TRPV3) ion channel protein and synovial cell apoptosis in rats with rheumatoid arthritis (RA), to provide a new basis for the anti-inflammatory mechanism of moxibustion.

Methods

A total of 50 Sprague-Dawley (SD) rats were divided into a normal group, a model group, moxibustion group I, moxibustion group II and moxibustion group III by complete randomization, with 10 rats in each group. Rats in the normal group were bred routinely, and rats in the model group were also bred routinely after successful modeling. After successful modeling, rats in moxibustion group I, II and III accepted consecutive moxibustion at Zusanli (ST 36) and Shenshu (BL 23) for 15 d, once a day, respectively 5 min, 20 min and 30 min for each session. The degree of paw edema was observed and recorded. Immunohistochemical assay was used to detect the protein expression of TRPV3 ion channel in dorsal root ganglia and spinal cord dorsal horn. Terminal-deoxynucleoitidyl transferase-mediated nick end labeling (TUNEL) was used to detect apoptotic synovial cell number.

Results

At the end of treatment, paw circumference of rats in moxibustion group II and III were significantly reduced as compared with that in the model group (P<0.05). TRPV3 ion channel protein expression of dorsal root ganglia and spinal cord dorsal horn was higher in the model group than that in the normal group (P<0.05); the TRPV3 ion channel protein expressions of dorsal root ganglia and spinal cord dorsal horn in moxibustion group II and III were higher than that in moxibustion group I (P<0.05); apoptotic synovial cell number in the model group was larger than that in the normal group (P<0.05), and apoptotic synovial cell numbers in moxibustion group II and III were significantly higher than that in the model group (P<0.05).

Conclusion

Moxibustion of appropriate time could induce TRPV3 expression, and promote synovial cell apoptosis.

     

卡前列素氨丁三醇联合卡孕栓在预防剖宫产产后出血及术后恢复中的疗效

目的:分析卡前列素氨丁三醇、卡孕栓联用在剖宫产产后出血预防和术后恢复中的应用。方法:收集108例在我院行剖宫产分娩的产妇,随机分2组,其中对照组在胎儿娩出后选用缩宫素和卡孕栓防治出血,而观察组则应用卡前列素氨丁三醇和卡孕栓,对比两组产妇产后出血以及术后恢复状况。结果:观察组产后2h出血量(104.69±28.42)ml、24h出血量(239.57±52.03)ml、首次肛门排气时间(26.41±8.84)h,均显著少于对照组的产妇(p0.05);产后3d,观察组子宫复旧良好率70.37%,对照组为66.67%,组间差异不明显(p0.05)。结论:卡前列素氨丁三醇联合卡孕栓应用于剖宫产产妇中能够显著减少产后出血量,预防大出血,且可有效促进产妇术后胃肠功能恢复。     

气道阻力测定在儿童支气管哮喘规范化治疗中的应用

目的 探讨气道阻力测定(Rint)在儿童支气管哮喘规范化治疗中的应用价值.方法 对36例规范化治疗的支气管哮喘患儿分别于急性发作期、慢性持续期进行气道阻力测定.结果 哮喘急性发作期Rint明显高于慢性持续期,而慢性持续期1个月、3个月气道阻力实测值百分比＞ 140％,6个月、1年气道阻力实测值百分比＜140％,哮喘急性发作期规范化吸入支气管舒张剂后Rint明显降低.结论 气道阻力测定可反映儿童哮喘肺功能改变,为哮喘规范化治疗提供客观有力依据.