都江堰市地震灾区人群应激口腔黏膜病损状况调查

[目的]了解地震灾区人群口腔黏膜病损状况,评估应激状况对口腔黏膜病损的相关性。[方法]采用随机整群抽样调查方法,以都江堰灾区平板房社区居住人群为调查对象,分别进行应激自评量表、口腔健康问卷调查和口腔健康检查。[结果]完成都江堰市灾区15个平板房社区共计970人的问卷调查和口腔健康检查,都江堰市地震灾区人群灾后有口腔黏膜病损病史和现患口腔黏膜病损者为11.4%,应激自评量表评分等级与灾后有口腔黏膜病损病史和现患口腔黏膜病损者的关联性有统计学意义(P﹤0.05)。[结论]都江堰市地震灾区人群的口腔黏膜病损十分普遍,加强灾区人群心理按抚是预防和控制口腔黏膜病损者的基本方法。     

浅析医院纪检监察信访工作

笔者结合医院纪检监察信访工作的分析数据,梳理了纪检监察信访工作的主要特点,提出要树立"一切为了病人"、"预防为先"、"迎难而上"的理念。针对信访工作中存在的不足,提出三点改进措施,即建立信访工作预警机制,完善组织架构和制度建设,建立医德医风、党纪党风教育的长效机制。     

高校附属医院腐败风险防控机制建设与实践

高校附属医院腐败风险防控机制建设借助全面质量管理理论和PDCA流程,建立了集预防、监督、处置于一体的腐败风险防控机制。营造廉洁的从医环境,强化教育、制度、惩处等环节的作用,实现了对腐败风险的动态管理。积极从源头上防治医药购销与医疗服务中的不正之风,推动了医院反腐倡廉建设。     

论新时期医院纪检监察部门的职能

医院纪检监察职能主要集中在教育、监督、查处、保护四个方面。本文通过服务理念的传播、顶层设计与规划、人文环境的营造、制度规范的建设对纪检检查职能进行全方位的改革与创新。     

富血小板纤维蛋白修复犬股骨髁骨缺损的微观结构研究

目的:通过建立beagle犬股骨髁的骨缺损模型,观察富血小板纤维蛋白(platelet-rich fibrin,PRF)诱导骨再生过程中新生骨的微观结构,评价PRF修复骨缺损的特点和优势,为PRF在诱导骨再生及颌骨重建的临床应用提供新的实验依据。方法:在实验犬股骨髁制备骨缺损,按Choukroun方法制备PRF膜,将PRF膜、Bio-Oss骨替代材料及自体骨骨松质随机填入3处骨缺损。12周、24周处死动物并取材,采用微焦点计算机断层摄影系统(microfocus computerizedtomography system,Micro-CT)检测分析新生骨的几何信息和结构信息。结果:PRF新生诱导骨骨小梁结构呈板状,厚度较厚,连续性和成熟度良好,数量稍少,接近自体骨组的成骨情况,优于Bio-Oss组;观察期内均数随时间延长,有向天然骨组衍变的趋势;PRF诱导成骨表现为向心性的特点。结论:PRF可诱导新生骨形成,成骨效果接近生理状态,表现为向心性成骨方式,具有良好的应用前景。     

β-磷酸三钙复合改良型富血小板纤维蛋白对兔成骨细胞增殖与分化的影响

目的 探讨β-磷酸三钙(β-TCP)复合改良型富血小板纤维蛋白(A-PRF)对兔成骨细胞增殖和分化的影响。方法 选取新西兰乳兔取颅顶骨制备成骨细胞,成年雄性新西兰大白兔取耳中央动脉血制备兔A-PRF。实验分为4组：A-PRF(A-PRF组)、β-TCP(β-TCP组)及β-TCP+A-PRF复合物(复合组)分别与成骨细胞共培养,空白组无材料仅培养成骨细胞。扫描电镜观察成骨细胞在不同材料上的形态结构,黏附、生长及增殖情况;细胞计数试剂盒8(CCK-8)定量分析4组成骨细胞增殖情况;ELISA检测Ⅰ型胶原蛋白(Col-Ⅰ)、碱性磷酸酶(ALP)和骨钙素(OCN)分泌情况;茜素红染色检测矿化结节的形成情况。结果 成骨细胞与材料复合培养后,电镜下见细胞在β-TCP表面和内部空隙中,以及A-PRF材料表面生长增殖。CCK-8结果显示,β-TCP组培养前5 d,细胞生长增殖受到抑制,其余3组细胞未见明显异常;随培养时间延长细胞增殖明显,培养过程中复合组和A-PRF组细胞数量明显高于β-TCP组(P<0.05)。成骨相关蛋白检测结果显示：Col-Ⅰ、ALP和OCN的分泌均随时间延长而增加,同一...     

临床运用升阳益胃汤验案二则

升阳益胃汤为李东垣所制定,出于《内外伤辨惑论》,临床适用范围广泛,导师常临证加减,功效显著。     

富血小板纤维蛋白骨诱导动物模型的建立

BACKGROUND: At present, many studies have focused on platelet-rich fibrin combined with other bone substitute materials in repair of peri-implant bone defects and sinus lifting, but there is still a lack of research  about the platelet-rich fibrin alone in repair of critical-size bone defects. OBJECTIVE: To compare the effect of three kinds of bone substitutes, platelet-rich fibrin, Bio-Oss bone substitute and autogenous cancellous bone, in repair of bone defects. METHODS: Four areas of cylindrical critical bone defects with a diameter of 6.0 mm and depth of 10.0 mm were prepared in the medial femoral condyle of beagle dogs. Three areas of bone defects were implanted with autologous platelet-rich fibrin, Bio-Oss bone substitutes and autologous cancellous bone, respectively. The remaining one area of bone defect was not implanted any substance, as control group. X-ray and Micro-CT detections in bone defect area were conducted after 12 weeks of surgery. RESULTS AND CONCLUSION: (1) X-ray: the density of platelet-rich fibrin group was more higher, but still slightly lower than that of the surrounding normal bone tissue; the density of the Bio-Oss bone substitute group was more lower, but still higher than that of the surrounding bone tissues; the density of autologous cancellous bone group was more higher, which was close to that of the surrounding bone tissues; circular low-density images were visible in the control group. (2) Micro-CT: platelet-rich fibrin group was similar with the autogenous cancellous bone group, and their density was slightly lower than that of the surrounding normal bone tissues; the trabecular bone exlibited a clear regular arrangement, and no obvious interface; the Bio-Oss bone substitute group showed a high density image with clear interface; the bone defect area was still clearly visible in the control group, with a low-density image. The bone volume fraction and trabecular number in the platelet-rich fibrin and autologous cancellous bone groups were both higher than those in the Bio-Oss bone substitute group (P < 0.05). These results demonstrate that platelet-rich fibrin and autogenous cancellous bone have a similar bone repair effect.