排序方式: 共有5条查询结果,搜索用时 0 毫秒
1
1.
抗旋毛虫单克隆抗体的制备及应用 总被引:1,自引:0,他引:1
抗旋毛虫单克隆抗体的制备及应用华西医科大学寄生虫学教研室胡少良,冯瑞元七十年代中期以来,单克隆抗体以其突出特点被迅速地推广到包括寄生虫学在内的许多领域。1984年,Ortega-Pierres等[1]以及其他学者率先制备了抗旋毛虫单克隆抗体并开始着手... 相似文献
2.
3.
作者选用1株高特异性抗旋毛虫单克隆抗体2G_8B_(10)H_2,建立了单克隆抗体竞争酶联免疫吸附试验(competitive ELISA,简称cELISA),并进行了初步应用。结果表明:20只日本大耳兔在感染旋毛虫后17天有35%呈现阳性,31天全部阳性.对其中5只感染兔的血清终点滴度观察了142天,了解特异性抗体的变化规律.该规律为病程估计提供了线索。另外,其他蠕虫感染兔血清29份无1例假阳性发生。可以认为,单克隆抗体cELISA是一种灵敏、特异的旋毛虫病免疫诊断方法。 相似文献
4.
5.
抗旋毛虫单克隆抗体竞争酶联免疫吸附试验... 总被引:1,自引:1,他引:0
Monoclonal antibody competitive ELISA was devised with a highly specific McAb (2G8) against T. spiralis. Among 20 rabbits infected with T. spiralis experimentally. 35% (7/20) were positive on the 17th day post infection (PI); 100% positive on the 31st day PI. No cross reaction was found with 29 sera of rabbits infected with other kinds of helminth. The dynamic antibody titer of five infected rabbits sera were studied with this method. The change may be helpful to judge the state of infection. In short, anti-T. spiralis McAb competitive ELISA can be regarded as a sensitive and specific immunological diagnostic method for T. spiralis infection. 相似文献
1