366例泌尿生殖道感染者支原体培养及药敏分析

非淋菌性尿道炎(NGU)是一组发病率较高的性传播性疾病.支原体是引起NGU的病原体之一.目前临床上难治性NGU越来越多,为了探讨其原因,更清楚地了解支原体感染及其对常用抗生素的药物敏感性,我们对的366例泌尿生殖道标本进行了支原体检测,现将结果报告如下.     

参芪冠心片治疗胸痹100例

１００例气虚血瘀型冠心病患者服用参芪冠心片后胸闷、胸痛、心悸、气短、乏力等症状大多得到改善或消失,该药并有降血压,降血脂的作用。疗效明显优于复方丹参片。     

柱前衍生RP-HPLC法同时测定天花粉中天门冬氨酸与谷氨酸的含量

目的:建立同时测定天花粉中天门冬氨酸与谷氨酸含量的方法。方法:采用柱前衍生反相高效液相色谱法。在碱性条件下,氨基酸与异硫氰酸苯酯反应,生成异硫氰酸苯-氨基酸的衍生物。色谱柱为Venusil-AA氨基酸分析柱(250mm×4.6mm,5μm),流动相A为0.05mol·L-1醋酸钠溶液(pH6.5)、B为乙腈-水(80:20,V/V),梯度洗脱,检测波长为248nm。结果:天门冬氨酸与谷氨酸的进样量分别在12.16～486.40ng(r=0.9996)与21.08～843.20ng(r=0.9997)范围内与各自峰面积积分值呈良好的线性关系;平均加样回收率分别为98.5%(RSD=1.43%,n=9)与98.8%(RSD=1.19%,n=9)。结论:本方法简单、准确、、重复性好,可用于天花粉中天门冬氨酸与谷氨酸的含量测定。     

白色丘疹样营养不良型大疱性表皮松解症1例

白色丘疹样营养不良型大疱性表皮松解症是营养不良型大疱性表皮松解症（epidemlolysis bullosa dystrophica,DEB）的一种类型,可为常染色体显性遗传和隐性遗传,少见散发病例。最近我科诊治1例,现报告如下。     

婴幼儿泪道冲洗探通的方法探讨

新生儿泪囊炎多是由于胎儿期泪管下端被先天性残存膜所封闭或被上皮碎屑堵塞所致使泪液排出困难,表现为泪溢,结膜囊有分泌物,严重的有泪囊区隆起,压迫泪囊区有粘液或脓性分泌物自泪点溢出。通常采用泪道反复冲洗加泪道探通术治疗,以冲破残存膜,使泪管恢复畅通,达到治疗的目的。但由于婴幼儿泪点小,且不合作,体位不能固定,造成反复进针,为操作者带来困难,易误伤小儿眼球,造成不良后果。通过对841例婴幼儿行泪道冲洗、泪道探通术,不断总结经验,掌握了泪道冲洗及探通技巧,现介绍如下：1临床资料1997年1月～1998年3月行泪道冲洗…     

全自动血培养系统检测730份需氧血培养结果分析

目的:评价全自动血培养系统BacT/Alert 120的临床应用情况.方法:回顾性分析BacT/Alert 120全自动血培养系统检测730例需氧血培养的阳性率,阳性检出时间,细菌种类以及假阳性、假阴性率.结果:730例需氧血培养分离到84株细菌,阳性率11.5%,最快阳性检出时间2小时,24小时内检出的阳性占66.7%,48小时内检出的阳性占86.9%,72小时内检出的阳性占97.6%.84株细菌分布于28个种属,假阳性率2.2%,未发现假阴性.结论:应用BacT/Alert 120全自动血培养系统提高了血培养的阳性率,缩短了培养时间,检出细菌种类多,减少污染机会,结果快速、准确.     

尿流式有形成分及干化学分析在尿路感染诊断中的应用评价

Objective To evaluate the clinical application of automated urine formed elements analyzer and/or urine dipstick analyzer for examination of urinary formed elements in screening urinary tract infection (UTI). Methods 148 fresh midstream clear-catch urine samples from the UTI patients and 284 fresh midstream clear-catch urine samples from non-UTI subjects were selected. Bacteria culture was performed for bacterial colony counting and identification. Bacteria counts ( BACT), yeast-like fungus and WBC were performed by UF-looOi automated urine formed elements analyzer. Leukocyte esterase test (LEU) and nitrite test (NIT) were performed by URISYS 2400 urine dipstick analyzer. We evaluated data obtained from urine dipstick analyzer, UF-1000i and combination of UF-1000i with urine dipstick analyzer and the results was compared with those obtained from quantitative bacterial culture. Then we evaluated the sensibility, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy. Results Among the 148 patients with UTI, the positive rate of the quantitative bacterial culture was 73.6% (109/148), the positive rate of LEU and NIT detected by dipstick test 26. 4% (39/148).There was significantly statistical difference between bacterial culture and strip test(χ2 = 55.68 ,P < 0. 05 ). The positive rate of urine flow cytometry by UF-1000i with either positive of BACT and WBC was 91.2%(135/148), which was higher than the positive rate of the quantitative bacterial culture. There was significant difference between two methods (χ2 = 14. 70, P < 0. 05 ). The positive rate of anyone positive among BACT, WBC, LEU and NIT was 94. 6% (140/148) when detected with combination of dipstick test and UF-1000i, which was higher than the positive rate of the quantitative bacterial culture. And there was significant difference between two methods (χ2 = 20. 45, P < 0. 05 ). The sensitivity of dipstick test was low (26. 4% ,39/148 ), and specificity was high ( 99. 3%, 282/284 ) . The sensitivity, specificity, positive predictive value, negative predictive value of BACT detected by UF-1000i in diagnosing urinary tract infection were 92. 6% ( 137/148 ), 39. 8% ( 113/284 ). 44. 5% ( 137/308 ) and 91.1% ( 113/124 ), respectively. If the dipstick test was combined with UF-1000i, the sensitivity, negative predictive value, specificity, positive predictive value and accuracy were 98.0% ( 145/148 ), 97.1% ( 100/103 ). 35.2% (100/284) ,44. 1% (145/329) and 56. 7% (245/432), respectively. Conclusions The combination of urine dipstick test and automated urine formed elements analyzer UF-1000i plays an important role in early diagnosis of UTI. And it has significant value in diagnosis of UTI, especially for the patients with negative bacterial cultures of urine sample.     

血浆置换在ABO血型不相容的亲属活体肾移植中应用效果评价

目的 观察血浆置换在ABO血型不相容亲属活体肾移植中清除血型抗体的临床应用效果.方法 回顾分析2017年7月～2019年1月本院54例行ABO血型不相容肾移植患者的临床特征及行血浆置换治疗后血型抗体效价变化情况,分析血浆置换治疗过程中不良反应发生情况.结果 52/54例患者血型抗体(包括IgG和IgM)效价成功降至术前...     

尿流式有形成分及干化学分析在尿路感染诊断中的应用评价

Objective To evaluate the clinical application of automated urine formed elements analyzer and/or urine dipstick analyzer for examination of urinary formed elements in screening urinary tract infection (UTI). Methods 148 fresh midstream clear-catch urine samples from the UTI patients and 284 fresh midstream clear-catch urine samples from non-UTI subjects were selected. Bacteria culture was performed for bacterial colony counting and identification. Bacteria counts ( BACT), yeast-like fungus and WBC were performed by UF-looOi automated urine formed elements analyzer. Leukocyte esterase test (LEU) and nitrite test (NIT) were performed by URISYS 2400 urine dipstick analyzer. We evaluated data obtained from urine dipstick analyzer, UF-1000i and combination of UF-1000i with urine dipstick analyzer and the results was compared with those obtained from quantitative bacterial culture. Then we evaluated the sensibility, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy. Results Among the 148 patients with UTI, the positive rate of the quantitative bacterial culture was 73.6% (109/148), the positive rate of LEU and NIT detected by dipstick test 26. 4% (39/148).There was significantly statistical difference between bacterial culture and strip test(χ2 = 55.68 ,P < 0. 05 ). The positive rate of urine flow cytometry by UF-1000i with either positive of BACT and WBC was 91.2%(135/148), which was higher than the positive rate of the quantitative bacterial culture. There was significant difference between two methods (χ2 = 14. 70, P < 0. 05 ). The positive rate of anyone positive among BACT, WBC, LEU and NIT was 94. 6% (140/148) when detected with combination of dipstick test and UF-1000i, which was higher than the positive rate of the quantitative bacterial culture. And there was significant difference between two methods (χ2 = 20. 45, P < 0. 05 ). The sensitivity of dipstick test was low (26. 4% ,39/148 ), and specificity was high ( 99. 3%, 282/284 ) . The sensitivity, specificity, positive predictive value, negative predictive value of BACT detected by UF-1000i in diagnosing urinary tract infection were 92. 6% ( 137/148 ), 39. 8% ( 113/284 ). 44. 5% ( 137/308 ) and 91.1% ( 113/124 ), respectively. If the dipstick test was combined with UF-1000i, the sensitivity, negative predictive value, specificity, positive predictive value and accuracy were 98.0% ( 145/148 ), 97.1% ( 100/103 ). 35.2% (100/284) ,44. 1% (145/329) and 56. 7% (245/432), respectively. Conclusions The combination of urine dipstick test and automated urine formed elements analyzer UF-1000i plays an important role in early diagnosis of UTI. And it has significant value in diagnosis of UTI, especially for the patients with negative bacterial cultures of urine sample.     

狼疮肾炎患者血清晚期蛋白氧化产物水平与疾病活动的相关性

目的：探讨氧化应激标志物晚期蛋白氧化产物（ AOPP）在狼疮肾炎（ LN）患者血清水平变化及其与LN疾病活动的相关性。方法 LN患者29例,根据肾小球滤过率（eGFR）水平分为eGFR≥60 mL／（min·1.73 m2）组16例、eGFR＜60 mL／（ min·1.73 m2）组13例。同期选择性别、年龄、体重匹配的健康对照者21例（对照组）,测定各组血肌酐（Scr）、尿素氮（BUN）、血白蛋白（ALB）、血沉（ESR）、C反应蛋白（CRP）、补体C3、C4以及24 h尿蛋白定量（24hUPQ）、eGFR;ELISA法测定各组患者的AOPP、丙二醛（ MDA）、抗dsDNA抗体;运用系统性红斑狼疮（SLE）疾病活动指数（SLEDAI）积分表对LN患者的疾病活动性进行评分。结果 LN组患者CRP、ESR、24hUPQ、抗dsDNA抗体、AOPP、MDA均较对照组明显升高,差异有统计学意义（P＜0.05）,C3、C4、eGFR较对照组降低,差异有统计学意义（P＜0.05）;LN患者eGFR＜60组ESR、MDA较eGFR≥60组明显升高,差异有统计学意义（P＜0.05）;而CRP、AOPP、抗dsDNA抗体、SLEDAI积分、24hUPQ均较eGFR≥60组升高,但差异无统计学意义（P＞0.05）;血清AOPP与MDA呈显著正相关（r＝0.942,P＜0.05）,与抗dsDNA抗体呈显著正相关（r＝0.982,P＜0.05）,与SLEDAI积分呈正相关（r＝0.653,P＜0.05）,与eGFR呈负相关（r＝－0.298,P＜0.05）。结论 LN患者血清AOPP水平明显升高,且随着肾功能损害而进一步升高,可作为一种评估LN氧化应激状态的指标。 AOPP与LN疾病活动性密切相关。