辨证治疗高度及三度房室传导阻滞11例

用辩证分型治疗高度及三度房室传导阻滞11例。结果：7例转为正常窦性心律，（三度3例，高度4例），平均恢复时间为7.3天；2例高度房室传导阻滞转为莫氏Ⅰ型，另2例三度房室传导阻滞治疗后心率，心律虽无改变，但症状明显改善，本组总有效率为81.82％。     

射频自凝刀治疗重度宫颈糜烂40例临床观察

目的探讨治疗重度宫颈糜烂的更有效方法。方法选择使用射频自凝刀治疗40例为观察组，c02激光治疗30例、微波治疗30例为对照组，将3组治疗结果进行比较。结果射频自凝刀治疗组治愈率明显高于对照组。结论射频自凝刀治疗重度宫颈糜烂是一种简单、安全、经济、效果好、值得临床推广应用的方法。     

抗人免疫球蛋白轻链单克隆抗体的临床应用——鼻咽癌EB病毒血清学诊断

作者报道用181医院研制的抗人免疫球蛋白轻链单克隆抗体,用免疫酶法检测病理确诊的鼻咽癌患者302例,与多克隆抗体IgA对比,发现:(1)抗壳抗原的抗体阳性率;前者为100％,后者为99.7％;几何平均滴度(GMT):前者为1:52.07,后者为1:47.29;②抗早期抗原的抗体阳性率:前者为66.9％     

抗人免疫球蛋白轻链单克隆抗体对鼻咽癌EB病毒血清学反应的探讨

本文报道用抗人免疫球蛋白轻链单克隆抗体(Ig轻链McAb)以免疫酶法应用于鼻咽癌EB病毒血清学检测。结果较满意。     

抗人血清型IgA单克隆抗体杂交瘤细胞系的建立和鉴定

本文使用从IgA骨髓瘤患者血清中提出的IgA,免疫BALB/c小鼠,采用常规细胞融合的方法,获得4株针对IgA的单克隆抗体杂交瘤细胞系。抗体效价培养上清为10~(-2),诱生腹水ELISA测定为10~(-1)～10~(-6),冻存6个月后复苏,腹水效价仍保持10~(-1)～10~(-6)。染色体分析为102,基本上是Sp2/0骨髓瘤细胞染色数68和小鼠脾细胞42之和,说明为杂交瘤细胞。专一鉴定:本McAb经ELISA测定证明,仅与IgA起反应,小鼠亚类测定证明为IgG_1本McAb与     

PRIIWARY CARDIOIVlYOPATHY CLINICAL ANALYSIS OF 74 CASES II. OBSTRUCTIVE AND RESTRICTIVE CARDIOMYOPATHY

14 cases of obstructive cardiomYOPathy and 8 cases of restrictive cardiomYOPathy are analyz- cd. Of the 14 cases of obstructive cardiomyo- pathy 3 hacl POSitive familial history. Typical angina pectoris oecurred In 3 and falnting oc- curred in Z cases. A grade II-III systolic mur- mur was audible in all, mostly at the left lower sternal border. Electrocardiogram displayed abnormal Q wave in 10 cases and left ventri- cular hypertrOPhy with strain in 7 cases. Left heart catheterization in 5 cases disclosed a signj- ficant systolic pressure gradient between the left ventricle and aonta at rest in 2 cases; in the other 3 cases systolic pressurp gradient oc- curred after injection of isopropylnoradrenaline. Left ventriculography revealed narrowing of tjic ventiricular caVity and thickening of the inter- ventricular septum and free ve玎tricular wall in 5 cases. Echocardiagraphy in 2 cases and pho- nocardiography with simultaneous administra- tlon of vasoactive drugs or pcrformance of phy- sical maneuvers in 5 cases all showed charac- teristic diagnostic features. Atlrencrgic blocker was given in 4 cases with satisfactory results. There was no fatality tluring hospitalization al- though all the paticnts had a long history of cardiac symptoms (averaging 0.8 years) on ad- mission. Of the 8 cases of restrictive cardiomyo- pathy, 7 showed symptoms and signs of cardiac tamponade on admission. Iloentgenologic exa- mination revealed marked enlargement of heart shadow in all cases. Electrocardiography dis- played signs oi myocardial clamage in most cases. Riight heart catheterization in 4 cases disclosed early diastolic dipping and Iate aug- mented diastolle plateau of the right ventricular pressure pulse. Autopsy was performed in one ca.se. Differential diagnoses of restrictive car- diomyopatby, constrictive pericarditis, congcs- tive cardiomyopathy, theumatic heart disease, cirrhosis of liver, endomyoeardial fibrosis and idiopathic mural endomyoeardial disease are emphasized. Treatment was usually cardiac glycosides and diuretics. Z patients died during hOSPltalization, the duration from the appea- rance of symptoms to death averaged 3.8 years.     

抗人IgK和Igλ链单克隆抗体的制备及临床初步应用

用初乳和血清IgA免疫Balb/c小鼠的脾细胞与SP_2/O和NS-1瘤细胞融合,以ELISA双抗原夹心法检测获得5株杂交瘤细胞。其中3株(1D1、5C3、11A7)分泌抗人游离和结合型λ轻链;2株(4G12、14A6)分泌抗人游离和结合型K轻链。4G12与结合型K链反应比游离K链好。这些细胞株在长达1年的培养中仍能稳定分泌特异性单克隆抗体,经液氮冻存10个月后杂交瘤细胞仍保持原有的特性。用固相抗原竞争ELISA试验表明,两个抗K链McAb是针对不同的抗原位点,而3株抗λ链则针对相同的抗原位点。用于检测副蛋白和本周氏蛋白具有很好的特异性和敏感性。把McAb4G12-HRP和1D1-HRP混合,用于检测-EB病毒早期抗原(EA)和壳抗原(VCA)抗体被证明是满意的,同时试用于抗核抗体的检测也获得了初步的结果。     

抗人IgMcAb应用S—P法对淋巴瘤与非淋巴瘤等病变的观察

非何杰金氏淋巴瘤Ig克隆型分析的报道,多用PAP和ABC法,我们以抗人Ig McAb轻链K(Kappa)和λ(Lambda),重链IgA、IgM、IgG等作I抗,引进美国Zy—med公司生产的链霉菌素亲生物蛋白—过氧化酶连结法药盒(简称S—P法),对B细胞淋巴瘤等肿瘤和其它病变进行对比观察。     

抗人血清型IgA单克隆抗体杂交瘤细胞系的建立和鉴定

有关抗人IgA单克隆抗体(McAb)的研制,国内已有抗人唾液和初乳IgAMcAb的报告。我们使用从IgA骨髓瘤病人血清中抽提的IgA,免疫BALB/C小鼠,采用常规的细胞融合方法,获得了4株针对IgAM-cAb细胞系.它所产生的McAbs,将代替常规的血清学方法(多克隆抗体),对原发性或继发性IgA增多或缺乏的多种疾病的诊断和研究.本文仅就该细胞系的建立的主要结果报告如下。材料、方法与结果①抗原制备和免疫方法:取IgA骨髓瘤病人血清,经ZnSo_4和(NH_4)_2SO_4沉淀,再经DEAE-22纤维