牙隐裂132例临床分析

牙隐裂 ,又称为不全牙裂 ,是一种临床常见的牙体非龋性疾病。由于初期隐裂的难诊断性 ,常常导致该病的误诊 ,而延误治疗时机。近年来我们通过对132名患者 16 5颗患牙进行观察 ,发现牙隐裂的临床表现和诊断是有规律可循的。经治疗后 0 .5～ 2a的随诊 ,效果满意 ,报告如下。1　临床资料1.1　一般情况　 132例患者中 ,男性 72例 ,女性 6 0例。年龄 18～ 2 7a 9例 ,2 8～ 37a 5 2例 ,38～ 4 7a 5 8例 ,4 8a以上 14例。全部患牙中 ,有 17例曾进行过调牙合治疗 ,5例作过脱敏治疗 ,其余未曾治疗过。隐裂牙发生在单侧者 10 2人 ,占患者总数的 77.3% …     

大鼠胰腺癌微环境诱导未成熟树突状细胞功能的变化

Objective To study the effect of pancreatic cancer microenvironment on maturation of dendritic cells ( DCs), and to investigate the mechanism by which tumors escape from immune recognition. Methods DCs were cultured with rmGM (40μg/L) ,rmIL-4 (40μg/L) ,and the supernatant of cell line AR42J cells was added on the day 6, then a large number of DCs was gained. DCs cultured and in-duced by routine methods were used as controls. The expression of surface molecules CD86, and CD80 in DCs was detected to observe whether this intervention could delay or block maturation of DCs and this effect could be reversed by stimulation with lipopolysaccharide (LPS). T cell stimulatory effects were also determined. Results Compared to normal DCs, CD86 and CD80 expression positive rate was significantly decreased in DCs induced by AR42J supernatant from (70.88±3.60)% to (7.15±0.71 )%. After stimulation with LPS, the expression of CD86, and CD80 in DCs was still low, suggesting the AR42J super-natant could block the maturation of DCs. The allostimulatory capacity of the immature DCs stimulated with the AR42J superoatant was significantly lower than that of the normal immature DCs. Conclusion The supernatant from pancreatic cancer ceils in rats could inhibit the maturation of DCs, which was not easily reversed.     

大鼠胰腺癌微环境诱导未成熟树突状细胞功能的变化

Objective To study the effect of pancreatic cancer microenvironment on maturation of dendritic cells ( DCs), and to investigate the mechanism by which tumors escape from immune recognition. Methods DCs were cultured with rmGM (40μg/L) ,rmIL-4 (40μg/L) ,and the supernatant of cell line AR42J cells was added on the day 6, then a large number of DCs was gained. DCs cultured and in-duced by routine methods were used as controls. The expression of surface molecules CD86, and CD80 in DCs was detected to observe whether this intervention could delay or block maturation of DCs and this effect could be reversed by stimulation with lipopolysaccharide (LPS). T cell stimulatory effects were also determined. Results Compared to normal DCs, CD86 and CD80 expression positive rate was significantly decreased in DCs induced by AR42J supernatant from (70.88±3.60)% to (7.15±0.71 )%. After stimulation with LPS, the expression of CD86, and CD80 in DCs was still low, suggesting the AR42J super-natant could block the maturation of DCs. The allostimulatory capacity of the immature DCs stimulated with the AR42J superoatant was significantly lower than that of the normal immature DCs. Conclusion The supernatant from pancreatic cancer ceils in rats could inhibit the maturation of DCs, which was not easily reversed.     

食指背侧岛状皮瓣急诊修复外伤性拇指指腹缺损

自１９９０年以来，我们采用食指背侧岛状皮瓣急诊修复外伤性拇指指腹缺损５６例，取得了满意的效果，今报告如下。１临床资料１．１病例资料本组共５６例。男３９例，女１７例。年龄２２～４３岁。电刨刨伤２３例，切割伤１７例，压砸伤１６例。受伤至手术时间２～５h，拇指指腹缺损范围（２．０～３．０cm）×２．０cm，深达骨质。均采用食指背侧岛状皮瓣修复，皮瓣面积，最大３．５cm×２．５cm，最小２．５cm×２．５cm。所有皮瓣均Ⅰ期成活。术后随访０．５～２．５年，皮瓣修复好的拇指指腹外观饱满，色泽正常，血循环丰富，出汗、感…     

开放性喉外伤51例救治体会

开放性喉外伤常可引起大出血、呼吸困难、休克等而危及生命。1980～1997年,我们救治此类伤51例,现将其救治体会报告如下。1　临床资料1.1　一般情况　男35例,女16例;年龄8～71岁,平均32.5岁。刀割伤21例,车祸伤12例,工伤7例,枪弹伤4例,爆炸伤4例,锐器刺伤3例。伤后至就诊24h内40例,1～7d8例,7d以上3例。1.2　临床表现　呼吸困难30例,其中～度17例。声嘶29例,吞咽困难20例,局部大出血8例。受伤部位:舌骨断裂4例,甲状舌骨膜破裂5例,会厌软骨裂伤、血肿6例,甲状软骨板断裂12例,环状软骨断裂9例,环甲膜破裂7例,声带、假声带、喉粘膜严重撕裂伤13…     

大鼠胰腺癌微环境诱导未成熟树突状细胞功能的变化

Objective To study the effect of pancreatic cancer microenvironment on maturation of dendritic cells ( DCs), and to investigate the mechanism by which tumors escape from immune recognition. Methods DCs were cultured with rmGM (40μg/L) ,rmIL-4 (40μg/L) ,and the supernatant of cell line AR42J cells was added on the day 6, then a large number of DCs was gained. DCs cultured and in-duced by routine methods were used as controls. The expression of surface molecules CD86, and CD80 in DCs was detected to observe whether this intervention could delay or block maturation of DCs and this effect could be reversed by stimulation with lipopolysaccharide (LPS). T cell stimulatory effects were also determined. Results Compared to normal DCs, CD86 and CD80 expression positive rate was significantly decreased in DCs induced by AR42J supernatant from (70.88±3.60)% to (7.15±0.71 )%. After stimulation with LPS, the expression of CD86, and CD80 in DCs was still low, suggesting the AR42J super-natant could block the maturation of DCs. The allostimulatory capacity of the immature DCs stimulated with the AR42J superoatant was significantly lower than that of the normal immature DCs. Conclusion The supernatant from pancreatic cancer ceils in rats could inhibit the maturation of DCs, which was not easily reversed.     

大鼠胰腺癌微环境诱导未成熟树突状细胞功能的变化

Objective To study the effect of pancreatic cancer microenvironment on maturation of dendritic cells ( DCs), and to investigate the mechanism by which tumors escape from immune recognition. Methods DCs were cultured with rmGM (40μg/L) ,rmIL-4 (40μg/L) ,and the supernatant of cell line AR42J cells was added on the day 6, then a large number of DCs was gained. DCs cultured and in-duced by routine methods were used as controls. The expression of surface molecules CD86, and CD80 in DCs was detected to observe whether this intervention could delay or block maturation of DCs and this effect could be reversed by stimulation with lipopolysaccharide (LPS). T cell stimulatory effects were also determined. Results Compared to normal DCs, CD86 and CD80 expression positive rate was significantly decreased in DCs induced by AR42J supernatant from (70.88±3.60)% to (7.15±0.71 )%. After stimulation with LPS, the expression of CD86, and CD80 in DCs was still low, suggesting the AR42J super-natant could block the maturation of DCs. The allostimulatory capacity of the immature DCs stimulated with the AR42J superoatant was significantly lower than that of the normal immature DCs. Conclusion The supernatant from pancreatic cancer ceils in rats could inhibit the maturation of DCs, which was not easily reversed.     

腹部手术后肠瘘20例治疗效果分析

<正>腹部手术后肠瘘是指腹部手术后由于医源性因素导致肠管之间、肠管与其他脏器之间或者体外出现病理性通道,造成肠内容物流出肠腔,引起感染、体液丢失、营养不良和器官功能障碍等一系列病理生理改变[1]。肠外瘘是腹部外科常见而严重的并发症,病死率较高,手术后肠瘘占所有肠瘘的75%⁸5%,也可由先天性畸形、创伤、炎症、肿瘤等引起。在20世纪60年代前其病死率为40%85%,也可由先天性畸形、创伤、炎症、肿瘤等引起。在20世纪60年代前其病死率为40%⁶0%。近来由于全胃肠外营养     

门脉高压患者血浆刺激血管内皮细胞eNOS与Hsp90相互作用的实验研究

目的：用门脉高压患者血浆刺激血管内皮细胞研究内皮型一氧化氮合酶（eNOS）与热休克蛋白90（Hsp90）的相互作用。方法：免疫共沉淀技术是研究蛋白质相互作用的一个有力工具。用肝硬化门脉高压患者血浆处理培养的人脐静脉血管内皮细胞（human umbilical vascular endothelial cell，HUVEC）后，在0、15、30、60和120min时收集细胞，利用该技术检测对Hsp90和eNOS相互作用的影响。细胞裂解液分别用抗eNOS抗体和抗Hsp90抗体沉淀蛋白复合体．经SDS～PAGE电泳和转膜后，再分别用抗Hsp90抗体和抗eNOS抗体进行免疫识别。结果：本实验分别检测出了eNOS和Hsp90的表达,说明Hsp90和eNOS存在相互作用的关系。Hsp90和eNOS对刺激产生的反应有时相性，一般在刺激30min后Hsp90的量有明显增加，进一步说明Hsp90对eNOS调节NO的产生起关键的作用。结论：门脉高压患者血浆可以刺激血管内皮细胞Hsp90与eNOS结合，增强eNOS的生物活性，使NO释放增多。