聚合酶链反应诊断女性外阴尖锐湿疣初步探讨

聚合酶链反应诊断女性外阴尖锐湿疣初步探讨郑惠方单祥年李明发蒋清龚嘉仪（南京铁道医学院附属医院妇产科，生物学教研室，附属医院病理科南京２１０００９）尖锐湿疣（ＣＡ）主要由人乳头瘤病毒（ＨＰＶ）６及１１型ＤＮＡ感染引起。尖锐湿疣和假性湿疣...     

Flow-induced Expression and Phosphorylation of VASPin Endothelial Cells

1 Introduction It is well known that mechanical forces have important influence on endothelial cells, in particular, on cytoskeleton reorganization. VASP (vasodilator stimulated phosphoprotein) is a 46 KD actin associated protein. It is a member of Ena/VASP protein family and composed of EVH1, proline-rich and EVH2 domains. It is considered as an important component of the sub-cellular regions where remodelling of the actin cytoskeleton takes place, such as the front of spreading lamell…     

响应面法优化白刺花总黄酮提取工艺研究

目的:优选白刺花中总黄酮的最佳提取工艺。方法:采用响应面分析法优化,以总黄酮提取率为评价指标,在考察单因素试验的基础上,选取乙醇浓度、加液量、提取温度和提取时间为自变量,利用Design-Expert软件分析优化出白刺花中总黄酮的最佳提取工艺条件。结果:优化所得白刺花中总黄酮的最佳提取工艺条件为:乙醇浓度为72%,加液量为21倍,提取温度为71℃,提取时间为133 min。结论:响应面优化所得到的最佳提取工艺准确、稳定,是提取白刺花中总黄酮的可行方法。     

急性呼吸窘迫综合征犬呼气相和吸气相的压力-容积曲线

目的研究急性呼吸窘迫综合征(ARDS)犬低容积段及常规压力-容积(P-V)曲线的特征,并探讨其产生机制。方法 16条犬前后自身对照。静脉注射油酸制造ARDS犬模型。用大注射器法绘制以功能残气量 (FRC)为基线的低容积段及常规呼气相和吸气相P-V曲线。结果 ARDS犬低容积段呼气相P-V曲线出现低位拐点(LIP,d),且是一段范围,压力为(5．56±0．49)-(7．67±0．54)cm H2O,容积为(244．44±102．15)- (307．69±118．75)mL。常规吸气相P-V曲线低位拐点(LIP)也是一段范围,压力为(7．77±0．88)～(9．25 ±0．85)cm H2O,容积为(261．36±94．45)～(310．42±96．21)mL,均高于LIP,d(P<0．05);高位拐点(UIP)的压力为(13．75±0．66)cm H2O;呼气相P-V曲线拐点(EIP)的压力为(10．67±0．82)cm H2O,低于UIP(P< 0．05)。CT示,ARDS犬仰卧位时上部肺组织通气尚正常,通气差及无通气肺组织主要位于中、下部。随着呼气末气道正压(PEEP)的加大,通气良好肺组织持续增加,其中在LIP,d位置显著增加。结论 ARDS犬的LIP是一段区间,标志着陷闭肺泡复张的开始,肺泡复张贯串于整个吸气过程。EIP标志着肺泡由过度扩张转化为正常开放状态。低容积段呼气相P-V曲线出现一段区间的LIP,d,标志陷闭肺区复张后逐渐重新陷闭。     

Chronic neuroprotective effects of low concentration lithium on SH-SY5Y cells:possible involvement of stress proteins and gene expression

To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed SH-SY5Y cells to 0.5 mmol/L lithium carbonate(Li2CO2) for 25–50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in SH-SY5Y cells. cDNA arrays showed that pyruvate kinase 2(PKM2) and calmodulin 3(CaM 3) expression levels were significantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II(CK2), threonine/tyrosine phosphatase 7(PYST2), and dopamine beta-hydroxylase(DBH) expression levels were significantly up-regulated. Besides, western blot analysis of stress proteins(HSP27, HSP70, GRP78 and GRP94) showed an over-expression of two proteins: a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modifications of proteins cited above.     

百令胶囊对频繁发作 ＣＯＰＤ 病人免疫功能和肺功能的影响

目的：观察百令胶囊对频繁发作慢性阻塞性肺疾病（ＣＯＰＤ）病人免疫功能和肺功能的影响。方法： 选取 １００ 例年发作次数在 ２ 次以上，每次发作均来本院呼吸内科住院诊治的慢性阻塞性肺疾病急性加重期病人 （ＡＥＣＯＰＤ）。５２ 例对照组病人在常规治疗的基础上增加无创正压通气（ＮＩＰＰＶ），４８ 例观察组病人在对照组之 上增加百令胶囊治疗。观察两组病人治疗前后免疫功能和肺功能情况。结果：观察组临床治疗有效率为８３．３３％ 显著高于对照组的６５．３８％，有统计学意义（χ２ ＝ ４．１８，Ｐ＜０．０５）；观察组治疗后 ＦＥＶ１ 、ＦＥＶ１ 预测值、ＦＥＶ１ ／ ＦＶＣ 均 高于对照组，差异有统计学意义（Ｐ＜０．０５）；观察组治疗后 Ｔ 淋巴细胞亚群 ＣＤ４＋、ＣＤ４＋ ／ ＣＤ８＋高于对照组，ＣＤ８ ＋低于对照组，均有统计学差异（Ｐ＜０．０５）。结论：采用百令胶囊辅助治疗，可改善病人免疫功能，提高肺功能，有 望降低 ＡＥＣＯＰＤ 发作次数。     

白血病抑制因子与支气管哮喘

在支气管哮喘(简称哮喘)的发病过程中,机体神经、免疫与内分泌系统均参与其中,它是一个多步骤的过程,涉及到大量的细胞因子、炎症介质和神经递质.白血病抑制因子(leukemia inhibitory factor,LIF)是连接神经-免疫-内分泌系统网络的桥梁,LIF通过调节多种炎症细胞,如嗜酸粒细胞、淋巴细胞和肥大细胞等,促进炎性介质的释放;促进气道感觉神经末梢释放P物质等神经肽,上调气道上皮细胞神经激肽-1受体(neurokinin-1 receptor,NK-1R)的表达,诱发气道神经源性炎症;此外,LIF还可下调糖皮质激素受体的表达,导致糖皮质激素抵抗.因此LIF水平在哮喘患者血清、痰液、支气管肺泡灌洗液、支气管黏膜中均可有所升高.本文就LIF的生物学特征以及与哮喘发病的关系作一综述,旨在为探讨哮喘的发病机制和临床治疗提供理论依据.     

呼吸监护病房老年患者高钠血症的临床分析

目的 探讨老年危重患者高钠血症与病情变化及预后的关系。方法 回顾2002年5月-2003年10月呼吸监护病房所有老年高钠血症患者临床资料．并用统计学方法对上述资料进行分析。结果 189例患者中高钠血症26例，发生率13．76％。高钠血症患者中死亡14例．病死率53．85％。在气管插管病例中高钠血症发生率为70．37％。发生高钠血症时急性生理学和慢性健康状况(APACHE)Ⅱ评分显著高于入监护室24小时的APACHEⅡ评分，同时肾功能也有一定影响，血肌酐值明显升高。高钠血症中死亡组与存活组比较，血钠水平有显著差异。结论 高钠血症是老年危重患者较常见的并发症，在气管插管患者发生率更高，且预后差。因此要重视老年危重患者高钠血症的发生。     

Interleukin 28B-related polymorphisms: A pathway for understanding hepatitis C virus infection?

AIM:To analyze the role of rs12979860 and rs8099917polymorphisms in hepatitis C virus(HCV)genotype 1infection of Brazilians.METHODS:A total of 145 adult patients diagnosed with genotype 1 chronic hepatitis C(CHC)who had completed a 48-wk regimen of pegylated-interferonα-2a or-2b plus ribavirin combination therapy were recruited from six large urban healthcare centers and199 healthy blood donors(controls)from a single site between January 2010 and January 2012.Data on the patients’response to treatment was collected.Polymerase chain reaction-restriction fragment length polymorphism genotyping of the interleukin(IL)28B gene fragment encompassing the single nucleotide polymorphisms(SNPs)rs12979860(C/T)and rs8099917(T/G)was carried out for 79 of the CHC patients and 199 of the controls.Bi-directional amplicon sequencing of the two SNPs was carried out for the remaining 66 CHC patients.RESULTS:SNP rs12979860 genotyping was successful in 99.5%of the controls and 97.2%of the CHC patients,whereas the SNP rs8099917 genotyping was successful in 95.5%of the controls and 100%of the CHC patients.The genotype and allele distributions for both rs12979860 and rs8099917 were significantly different between the control and CHC patient groups,with significantly higher genotype frequencies of CC and TT in the controls(P=0.037 and 0.046,respectively)and of TT and GG in the CHC patients(P=0.0009and 0.0001,respectively).Analysis of the CHC patients who achieved sustained virological response(SVR)to treatment(n=55)indicated that the rs12979860 C allele and CC genotype were predictors of SVR(P=0.02).No significant correlation was found between rs8099917 genotypes and treatment response,but carriers of the T allele showed significantly higher rates of SVR(P=0.02).Linkage disequilibrium analysis of the group that achieved SVR showed a significant association between rs12979860 and rs8099917(P=0.07).CONCLUSION:The higher allele frequency of rs12979860 C and rs8099917 T observed in non-HCVinfected individuals may indicate a potential pro     

复方甲氧那明治疗感染后咳嗽的有效性和安全性多中心临床研究

目的 评价复方甲氧那明治疗感染后咳嗽的有效性和安全性.方法 采用开放的、前瞻性多中心临床研究.病例选自2009年11月至2010年10月在6家教学医院呼吸科就诊,并被诊断为感染后咳嗽的患者 入选时咳嗽症状积分至少≥2分.治疗用药为复方甲氧那明胶囊(第一三共制药(上海)有限公司生产 商品名:阿斯美)2粒 3次/d 饭后口...