外固定架治疗桡骨远端粉碎性骨折

目的总结桡骨远端粉碎性骨折的外固定架治疗疗效。方法从1995年～2003年采用外固定架治疗桡骨远端粉碎性骨折46例,并进行随访。结果治疗后均随访1年,术后按Dienst评分,优良率为83．3％。结论外固定架固定牢靠,可早期活动腕关节,是治疗桡骨远端粉碎性、开放性骨折的有效方法。     

4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.     

4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.     

肝硬化合并多脏器衰竭的临床特点

198 5～ 1999年 ,我院共收治肝硬化失代偿期患者 5 89例 ,其中 14 5例合并多脏器衰竭 (MOF)。现报告如下。资料分析 :本组 14 5例肝硬化合并 MOF患者 ,男 90例 ,女 5 5例 ;年龄 2 3～ 65岁 ,平均 48.5岁 ;病程 2～ 2 5年。其中肝炎后肝硬化 14 2例 ,酒精性肝硬化 2例 ,胆汁性肝     

复合二氧化氯消毒饮用水副产物含量分析

目的了解复合二氧化氯消毒饮用水产生的副产物含量及其影响因素,探讨饮用水中消毒副产物含量变化规律。方法对2008-2013年复合二氧化氯消毒的出厂水和管网末梢水的亚氯酸盐、氯酸盐、三氯甲烷等指标进行检测分析。结果出厂水和管网末梢水的各项检测指标合格率以及各消毒副产物的均值差异均无统计学意义(P值均0.05);二氧化氯、耗氧量与消毒饮用水产生的副产物亚氯酸盐含量相关。结论复合二氧化氯消毒饮用水避免了有机副产物三卤甲烷污染的卫生问题,但还需提高二氧化氯转化率,并优化投加方式。     

腹腔镜下腹腔灌洗引流对重症急性胰腺炎全身炎症反应的影响

目的:探讨腹腔镜下置管腹腔灌洗引流(LPLD)对重症急性胰腺炎(SAP)全身炎症反应的影响.方法:37例SAP患者按入院先后顺序随机分为内科组和LPLD组.检测并比较两组治疗前后0、1、3、7、10 d外周血内毒素、TNF-α、IL-lβ、IL-6的浓度及单核细胞NF-κB的活性.结果:第0天 LPLD组内毒素、TNF-α、IL-lβ、IL-6浓度及单核细胞NF-κB活性与内科组比较无显著差异;术后第10天,LPLD组内毒素、TNF-α、IL-lβ、IL-6浓度及单核细胞NF-κB活性分别为(1.64±0.13)pg/mL、(114.13±15.12)pg/mL、(80.17±18.06)pg/mL、(56.43±12.24)pg/mL、(68.2±1.7)%.上述指标与内科组比较均有显著下降(P<0.01 or P<0.05).结论:LPLD能明显减轻内毒素血症,减低NF-κB活性及细胞因子浓度,有效地减轻SAP患者的炎症反应.     

中西药保留灌肠治疗溃疡性结肠炎96例

一般资料：本组男52例，女44例。年龄24～73岁，平均39．4岁，均经纤维结肠镜，乙状结肠镜或钡灌肠检查，其诊断符合1993年全国太原慢性非感染性肠道疾病学术研讨会所规定的溃疡性结肠炎诊断标准。其中，初发型38例，慢性复发型44例，慢性持续型14例，病程1～5年。治疗方法；病人确诊后，除阳其卧床休息，易消化低纤维素饮食及对症，支持治疗外，用l％奴夫卡因30ml，庆大霉素24万u，地塞米松10mg，锡类散0．6g，黄连素0．sg（研成粉末），生理盐水50ml，充分混合，加温至37C左右，嘱患者臀部抬高30cm后灌肠，半小时后臀部放平，侧卧休息，…     

卫生防疫机构在创建国家卫生城市工作中的作用

国家卫生城市称号．是一个城市的生活环境、文明程度和居民素质全面达到国家卫生城市标准的重要标志，它对于优化环境、改善人民居住生活条件具有现实意义。为此，各级政府都把创建国家卫生城市作为一项造福人民的工程，列入了工作日程。在这项工作中．卫生防疫机构承担着繁重的任务．如何在创建卫生城工作中利用自己的优势，充分发挥作用．是目前形势下值得探讨的问题。     

中成药要保持稳定的质量

采用传统的方法所生产的中成药，其质量不够稳定，可影响到疗效和出口，要保证中成药质量的稳定，首先要选择、有一定新鲜度的优良品种的原料药材，并且在炮制加工过程中要有规范化的操作工艺，在提取过程中要定量化，使中成药质量基本要求保持稳定。     

用正交设计法选择锻淬磁石的最佳工艺

磁石为天然磁铁矿石,质地坚硬,这种矿物药在调配处方前须经过炮制才能使质地酥脆而易煎出有效成分。常用的方法为火煅醋淬。历代文献记载要反复煅淬,操作起来较麻烦,费时费功。磁石究竟在多大温度下煅烧?煅烧几次?醋淬几次?目前淌未见报。下面我采用正交设计法,