人体入紫菜后尿中砷化物的排泄规律研究

目的 观察正常人摄人紫菜后,尿中不同形态砷化物随时间的排泄规律.方法 收集24名受试者(14名男性,10名女性)食人紫菜后72 h内不同时点的尿液,采用高效液相色谱.氢化物发生.原子荧光分光光度法(HPLC-HG-APS)检测尿中砷代谢产物的形态与浓度.结果 在食用紫菜后72 h内,与食用紫菜前比较,男性除第1和48 h外,尿中iAs浓度均显著增高(P<0.05);除第1和12 h外,尿中一甲基胂酸(MMA)浓度显著增高(P<0.05);第1～72 h尿中二甲基胂酸(DMA)浓度显著增高(P<0.05);女性受试者食用紫菜后,除第3、5和48 h外,尿中iAs浓度明显增高(P<0.05);除第5和12 h,尿中MMA浓度均显著增高(P<0.05);第1～72 h尿中DMA浓度均有显著增高(P<0.05).食用紫菜后,女性尿中DMA浓度显著高于男性.结论 摄入相同剂量紫菜后女性尿中DMA浓度显著高于男性,并且DMA浓度随时问先升高后降低.

Abstract：

Objective To know the effects of laver intake on arsenic species in urine of people, and investigate the characteristics of arsenic metabolism. Methods The urine samples were collected from 24 subjects who had laver available on markets, arsenic species in urine samples were detected with hydride generation atomic fluorescence spectroscopy. Results There was a significant increase of urinary iAs seventy-two hours after laver intake except one and forty-eight hours, increase of MMA seventy-two hours after laver intake except one and twelve hours, and increase of DMA from the first hour to the 72nd hour in male group. There was a significant increase in the urinary iAs seventy-two hours after laver intake except three, five and forty-eight hours, increase of MMA seventy-two hours after laver intake except five and twelve hours, increase of DMA from one to seventy-two hours after laver intake in female group. Conclusion The urinary concentration of DMA is significantly higher in female group compared with male group after laver intake in the same weight and urinary DMA increases in the beginning, after that decreases gradually.     

以腓动脉终末降支为蒂的外踝上皮瓣的解剖及临床应用

以腓动脉终未降支为血管蒂的外踝上皮瓣,修复足底跟骨外露1例,足背内踝肌腱骨质外露1例,单纯足背肌腱距骨外露2例,获得满意效果。此皮瓣较薄,有神经支配,血管蒂长,解剖位置恒定,切取容易,不损伤小腿外侧和后侧肌群的附着点,亦不牺牲腓动、静脉主干,临床有使用价值。     

2-甲基-1,2,5,6-四氢-5,6-二氧-1,2,4-三嗪-3-硫醇的合成研究

目的　制备 2 -甲基 - 1,2,5,6 -四氢 - 5,6 -二氧 - 1,2,4 -三嗪 - 3-硫醇。方法　在甲醇的碱性介质中,通过1-甲基 - 1-氨基硫脲与草酸二乙酯的缩合反应制备医药中间体三嗪环。结果及结论　适宜的反应条件为 1-甲基 - 1-氨基硫脲、草酸二乙酯、甲醇钠的量(摩尔)比例为 1 0∶1 1∶2 0,其收率可达 6 0 %。