微创经皮手术与开放手术椎弓根钉内固定治疗胸腰椎骨折的临床疗效对比

为了对比微创经皮手术与开放手术椎弓根钉内固定治疗胸腰椎骨折的临床疗效,收集了104例胸腰椎骨折患者的临床资料进行回顾性分析。患者均采取椎弓根钉内固定术治疗,按采取的手术方法分为微创经皮手术组和开放手术组。所有患者术后均获得12~18个月的随访。结果显示,微创经皮手术组的切口长度、手术时间、出血量、术后3天VAS评分和不良切口愈合率均显著低于开放手术组,但术中X线透视次数显著多于开放手术组;两组术后1年骨折椎体前缘高度比值、Cobb''s角、Oswestry功能障碍指数和内固定失败率无显著差异(P＞0.05)。因此微创经皮手术与开放手术椎弓根钉内固定治疗胸腰椎骨折,都可以获得满意的临床疗效。前者虽然存在较多的X线暴露的缺点,但创伤更小,适合在临床上推广应用。     

CTP-Ub-HBcAg原核表达载体的构建及其表达

目的构建CTP-Ub-HBcAg原核表达载体并诱导和鉴定其融合蛋白的表达。方法以质粒pcDNA3.1(-)-Ub-HBcAg中的Ub-HBcAg融合基因为模板设计引物,上游引物带有CTP基因序列,进行PCR扩增,PCR产物克隆到原核表达质粒pMAL-c2x中,菌落PCR阳性克隆测序鉴定,鉴定正确的质粒转化大肠杆菌BL21(DE3)诱导表达,并进一步通过直链淀粉树脂亲和层析法纯化融合蛋白,Western blotting鉴定。结果 PCR扩增得到820 bp大小的条带,为CTP-Ub-HBcAg融合序列。该序列被克隆至表达质粒pMAL-c2x中,阳性克隆菌落测序正确,并在DE3中获得诱导表达。Western blotting鉴定为70 KD的CTP-Ub-HBcA融合蛋白。结论构建CTP-Ub-HBcAg原核表达载体并成功表达,为进一步研究该融合蛋白的功能提供了实验基础。     

Polarity-dependent effect of low-frequency stimulation on amygdaloid kindling in rats

BackgroundLow-frequency stimulation (LFS, <5 Hz) has been proposed as an alternative option for the treatment of epilepsy. The stimulation pole, anode and cathode, may make different contributions to the anti-epileptic effect of LFS.ObjectiveTo determine whether electrode polarity influences the anti-epileptic effect of LFS at the kindling focus in amygdaloid kindling rats.MethodsThe effect of bipolar and monopolar (or unipolar) LFS at the amygdala in different polarity directions on amygdaloid kindling acquisition, kindled seizures and electroencephalogram (EEG) were tested.ResultsBipolar LFS in the same direction of polarity as the kindling stimulation but not in the reverse direction retarded kindling acquisition. Anodal rather than cathodal monopolar LFS attenuated kindling acquisition and kindled seizures. Bipolar LFS showed a stronger anti-epileptic effect than monopolar LFS. Furthermore, anodal LFS (both bipolar and monopolar) decreased, while cathodal LFS increased the power of the EEG from the amygdala; the main changes in power were in the delta (0.5–4 Hz) band, which was specifically increased during kindling acquisition.ConclusionsOur results provide the first evidence that the effect of LFS at the kindling focus on amygdaloid kindling in rats is polarity-dependent, and this may be due to the different effects of anodal and cathodal LFS on the activity in the amygdala, especially on the delta band activity. So, It is likely that the electrode polarity, especially that for anodal current, is a key factor affecting the clinical effects of LFS on epilepsy.     

Hemoglobin-Induced Nitric Oxide Synthase Overexpression and Nitric Oxide Production Contribute to Blood–Brain Barrier Disruption in the Rat

Hemoglobin (Hb) released from extravasated erythrocytes may have a critical role in the process of blood–brain barrier (BBB) disruption and subsequent edema formation after intracerebral hemorrhage (ICH). Excessive nitric oxide (NO) production synthesized by nitric oxide synthase (NOS) has been well documented to contribute to BBB disruption. However, considerably less attention has been focused on the role of NO in Hb-induced BBB disruption. This study was designed to examine the hypothesis that Hb-induced NOS overexpression and excessive NO production may contribute to the changes of tight junction (TJ) proteins and subsequent BBB dysfunction. Hemoglobin was infused with stereotactic guidance into the right caudate nucleus of male Sprague Dawley rats. Then, we investigated the effect of Hb on the BBB permeability, changes of TJ proteins (claudin-5, occludin, zonula occludens-1 (ZO-1), and junctional adhesion molecule-1 (JAM-1)), iron deposition, expression of inducible NOS (iNOS) and endothelial NOS (eNOS), as well as NO production. Hb injection caused a significant increase in BBB permeability. Significant reduction of claudin-5, ZO-1, and JAM-1 was observed after Hb injection as evidenced by PCR and immunofluorescence. After a decrease at early stage, occludin showed a fivefold increase in mRNA level at 7 days. Significant iron deposition was detectable from 48 h to 7 days in a time-dependent manner. The iNOS and eNOS levels dramatically increased after Hb injection concomitantly with large quantities of NO released. Furthermore, enhanced iNOS or eNOS immunoreactivity was co-localized with diffused or diminished claudin-5 staining. We concluded that overexpressed NOS and excessive NO production induced by Hb may contribute to BBB disruption, which may provide an important potential therapeutic target in the treatment of ICH.     

六安市2010至2015年流感病原学检测及流行病学分析

目的 分析六安市2010~2015年流感监测数据,探讨六安市近6年流感流行特征。方法 收集2010年1月至2015年12月六安市人民医院提供的15 435流感样病例资料,对其中3 890份采样标本进行流感病原学检测和病毒核酸检测,并进行亚型鉴定,同时收集哨点医院流感样病例（ILI）相关资料,运用描述性流行病学方法对资料进行流行病学分析。结果 共采集ILI咽拭子标本3 890份,检出流感核酸阳性422份,总阳性率为10.85%。分析流感病毒亚型,季H3 229份（54.27%）,新甲H1 53份（12.56%）,A未分型29份（6.87%）,B型109份（25.83%）。流感病毒检测阳性率随季节呈明显双峰,高峰分别为7~9月和12月至次年3月。0~5岁和25~60岁年龄组阳性率占比均为23.70%。男女阳性率占比差异无统计学意义（P>0.05）。结论 2010~2015年,六安市流感流行以季H3为主,B型流感年度分布比较均匀。流感流行时间呈双峰,分别在7~9月和12月至次年3月。5岁以下和25~60岁年龄组患病人数较多。     

基于中医“治未病”理论的健康管理在非酒精性脂肪肝中的应用效果评价

目的:评价基于中医"治未病"理论的健康管理在非酒精性脂肪肝中的应用效果。方法:选取2018年5月~2019年4月收治于我院的258例非酒精性脂肪肝患者作为研究对象,采用随机数字表法将258例患者随机分为观察组(129例)和对照组(129例)。对照组采用常规治疗方案,观察组在对照组基础上采取基于中医"治未病"理论的健康管理,比较两组治疗6个月后的总有效率、治疗前后单项症状积分、个体参数(体质量指数、腰臀比、收缩压、舒张压)、血脂指标(甘油三酯、总胆固醇),以及肝功能指标(谷丙转氨酶、谷草转氨酶)。结果:经治疗后,观察组患者的总有效率明显高于对照组,差异有统计学意义(P<0.05);且观察组单项症状积分均明显低于对照组(P<0.05),个体参数均低于对照组(P<0.05),血脂和肝功能指标水平均低于对照组(P<0.05)。结论:基于中医"治未病"理论的健康管理在脂肪肝中的应用可提高临床治疗效果,降低患者血脂,改善患者临床症状和肝功能,具有较好的应用效果。     

泛素化及自噬参与蛋白降解及抗原提呈的研究进展

细胞内所有的蛋白质都在不断的被降解,并被新合成的蛋白质所取代。真核细胞内主要有两条蛋白降解途径,分别为泛素一蛋白酶体系统途径和白噬途径,两条途径都在维持细胞内稳态方面起着重要的作用。泛素-蛋白酶体系统是由泛素介导的一种高度复杂的蛋白降解机制,它通过一种特定的方式降解并再循环利用细胞内的蛋白,而自噬是一种自我降解过程,它通过溶酶体介导的方式降解蛋白。被降解的蛋白可以被提呈至MHCⅡ类分子或MHCⅠ类分子,从而激发有效的T细胞反应。此文就这两个途径参与蛋白降解及抗原提呈的相关研究进展进行综述。     

转录因子Sp1在肝细胞癌中的表达

目的通过研究转录因子Sp1在肝细胞癌（HCC）组织中的表达及转化生长因子（TGF）B1对肝癌细胞中Sp1mRNA转录表达的影响,探讨Sp1在HCC中表达的意义及其在细胞凋亡中的作用。方法免疫组织化学方法对51例HCC组织与10例良性病变而切除的正常肝组织中转录因子Sp1进行蛋白检测;体外培养肝癌细胞株HepG2,施加外源性TGFβ1,逆转录一聚合酶链反应方法检测HepG2细胞中Sp1mRNA的表达,并用流式细胞仪检测细胞受到干预后的凋亡情况。结果Sp1蛋白在HCC组织中的表达率明显高于正常肝组织（P〈0．01）;施加外源性TGFβ1与对照组相比HepG2细胞Sp1mRNA的表达显著降低（P〈0．01）,且HepG2细胞凋亡率随着TGF[31浓度的增高而上升。结论Sp1蛋白在HCC中的过表达在肿瘤发生发展中起一定作用;TGFβ1诱导肝癌细胞凋亡,而Sp1基因的抑制在TGFβ1诱导肝癌细胞凋亡中可能起一定作用。