Response letter for "Mesenchymal stem cell secretome to control inflammation in allergic conjunctivitis"

Dr.Spampinato gave an insightful comment on "Mesenchymal stem cell secretome to control inflammation in allergic conjunctivitis" (1).Dr.Spampinato proposed that it is key to get the complete knowledge of mesenchymal stem cell (MSC) secretome composition in order to better understand MSC secretome and to achieve the best therapeutic effect of MSC secretome for clinic setting.This comment is highly significant and important.     

Transient Macular Edema after Laser In-Situ Keratomileusis

Purpose: To investigate the macular changes induced by laser in situ keratomileusisc(LASIK) procedure.Methods: Optical Coherence Tomography (OCT) was used to examine 27 eyes of 15LASIK patients before surgery, and 1 day, 1 week, 1 month after surgery.Results: The mean thick iness of neuroepithelial layer of macula was (195 ±24)microns, (178± 16) microns 1 day and 1 month after surgery, respectively, while thebaseline was (174 ± 12) microns. The increase in macular neuroepithelial thickness wascorrelated with the degree of myopia, but not with the duration of suction or thepostoperative BCVA.Conclusion: Mild macular edema is common in the first month following LASIK,especially in high myopia. However, no association with loss of BCVA has beenestablished. Eye Science 2003; 19: 20 - 24.     

肝内胆管结石的诊断和治疗

肝内胆管结石病首由香港Disby于1930年描述，以及日本等东亚国家相继有报告。我院外科于1942年12月施行急症胆总管探查发现了胆管蛔虫和脓性胆管炎，做了胆管取虫和T管引流术。1945年3月病理科尸检发现肝内胆管结石、胆管蛔虫，多发性肝脓肿和肝内门静脉支内胆沙栓塞等病变，打开了认识原发性胆管结石和东方常见的化脓性胆管炎之窗。现在，虽然在肝内结石的成因研究、诊断和治疗上已取得很大进步，但由于肝内结石分布的多样性致残石率较高及其合并症的严重性，因而仍为外科一个常见的难治病。1肝胆管结石的种类和病因1．l原发性肝胆管胆固…     

Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells

Purpose: To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma. Material and Methods: The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telormarse was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis.Results: Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24h and 1μg/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase.Conclusions: CDDP is an effective telomerase inhibitor in cultured choroidal melan     

RETlNAL VEIN OCCLUSION TREATMENT WITH WESTERN MEDICINE AND TRADITIONAL CHINESE MEDICINE

This paper presents 205 ca.ses of retinal vein occlusion (RVO) treated rr.wording t0 3 regi- mens and followed up periodically during the past 14 years. The clinical findings are analyz- ed. The effective rate of combined treatnwat with traditional Chinese medicine (TCM) and westeirn medicine in retinal branch vein oicclu- sion (RBVO) is 85.18To. 31.76'70 0f the patients with central retinal velin occlus.ion and 53.04% of tho相似文献   

IMMUNOLOGICAL STUDIES ON THROMBOANGIITIS OBLITERANS

Using humoral immunity (gamma-globulin 18 cases, IgG, IgA, IgM each 30 cases, CH50, C3, C4 each 29 cases and CIC 31 cases), cellular immunity (E-RFC 68 cases, As 30 cases, SmIg, SmIgG each 18 cases, IMIT 30 cases) and immunopathological manifestations (light microscope, fluorescence microscope, transmitted electron microscope each 7 cases) as indices, we studied the immunological changes of "progressing", "remittent" and "stabilized" groups of patients with thromboangiitis obliterans (TAO) in different stages. Humoral immunity indicated that gamma-globulin, immune complex and IgG were all increased; cellular immunity indicated that the rate of T cells and suppressor cells was declined, while that of B cells was elevated. Immunopathologically, under light microscope all the layers of involved vessels were infiltrated with neutrophils, lymphocytes and monocytes; under fluorescence and electron microscope, immune complexes were found in the involved vessel walls. Our preliminary results suggest that TAO is an autoimmune disease relevant to antigen-antibody complex.     

A comparison of two data analyses from two observer performance studies using Jackknife ROC and JAFROC

The authors compared two methodological approaches, Jackknife ROC and JAFROC, in analyzing data ascertained during FROC (free-response receiver operating characteristics) type studies. Observer rating data obtained from two observer performance studies were analyzed. During the first study, seven radiologists interpreted 120 mammography examinations depicting 57 masses under five different conditions with and without the results of computer-aided detection (CAD). In the second study, eight radiologists interpreted 110 examinations depicting 51 masses under six different display conditions with and without CAD results. Readers rated the detection task in a FROC type response. Jackknife ROC (using the software of LABMRMC with the highest rating per case) and JAFROC were used to compute differences, if any, in summary performance levels among all reading modes in each study as well as for all paired data sets. The results of the different analytical approaches are compared. The overall results for all modes were significantly different for the first study (p < 0.05) and not significant (p > 0.05) for the second study using either analytical approach. In the first study, the performance levels represented by three paired data sets were significantly different (p < 0.05) when computed using LABMRMC and four pairs were significantly different (p < 0.05) using JAFROC. In eight of ten pairs, JAFROC produced lower p values than LABMRMC. In the second study, LABMRMC showed no significant differences for any paired data sets and JAFROC showed a significant difference for one pair. In 15 of 16 pairs, p values computed by JAFROC were lower than those computed by LABMRMC.     

SARS coronavirus induces apoptosis in Vero E6 cells

Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.