Analysis on internal mechanism of zedoary turmeric in treatment of liver cancer based on pharmacodynamic substances and pharmacodynamic groups

Zedoary tumeric (Curcumae Rhizoma, Ezhu in Chinese) has a long history of application and has great potential in the treatment of liver cancer. The anti liver cancer effect of zedoary tumeric depends on the combined action of multiple pharmacodynamic substances. In order to clarify the specific mechanism of zedoary tumeric against liver cancer, this paper first analyzes the mechanism of its single pharmacodynamic substance against liver cancer, and then verifies the joint anti liver cancer mechanism of its "pharmacodynamic group". By searching the research on the anti hepatoma effect of active components of zedoary tumeric in recent years, we found that pharmacodynamic substances, including curcumol, zedoarondiol, curcumenol, curzerenone, curdione, curcumin, germacrone, β-elemene, can act on multi-target and multi-channel to play an anti hepatoma role. For example, curcumin can regulate miR, GLO1, CD133, VEGF, YAP, LIN28B, GPR81, HCAR-1, P53 and PI3K/Akt/mTOR, HSP70/TLR4 and NF-κB. Wnt/TGF/EMT, Nrf2/Keap1, JAK/STAT and other pathways play an anti hepatoma role. Network pharmacological analysis showed that the core targets of the "pharmacodynamic group" for anti-life cancer are AKT1, EGFR, MAPK8, etc, and the core pathways are neuroactive live receiver interaction, nitrogen metabolism, HIF-1 signaling pathway, etc. At the same time, by comparing and analyzing the relationship between the specific mechanisms of pharmacodynamic substance and "pharmacodynamic group", it is found that they have great reference significance in target, pathway, biological function, determination of core pharmacodynamic components, formation of core target protein interaction, in-depth research of single pharmacodynamic substance, increasing curative effect and so on. By analyzing the internal mechanism of zedoary tumeric pharmacodynamic substance and "pharmacodynamic group" in the treatment of liver cancer, this paper intends to provide some ideas and references for the deeper pharmacological research of zedoary tumeric and the relationship between pharmacodynamic substance and "pharmacodynamic group".     

电针“足三里”穴对脾气虚大鼠小肠黏膜转运体SGLT1及GLUT2表达的影响

目的:观察电针双侧"足三里"穴对脾气虚模型大鼠小肠黏膜上皮组织内的钠依赖葡萄糖转运体(SGLT1),葡萄糖运载蛋白2(GLUT2)的基因及蛋白表达的影响。方法:将40只SD雄性大鼠随机分为正常组、脾气虚组、足三里组、非经非穴组,每组10只。所有大鼠均在SPF级动物实验中心饲养。除正常组外,其余3组均采用劳倦过度和不规则饮食复合法建立脾气虚证的大鼠模型。造模成功之后,对足三里组、非经非穴组大鼠分别进行电针"足三里"穴、非经非穴点干预处理7天。采用HE染色方法观察各组大鼠小肠黏膜组织形态变化;采用荧光定量PCR法检测大鼠小肠黏膜上皮的SGLT1和GLUT2的mRNA表达水平;采用蛋白质免疫印迹法(WB)检测大鼠小肠黏膜上皮组织内的SGLT1和GLUT2的蛋白含量变化。结果:脾气虚组大鼠小肠黏膜组织部分损伤,足三里组的小肠黏膜组织有一定程度的恢复;脾气虚组和非经非穴组大鼠小肠黏膜组织内的SGLT1和GLUT2蛋白和基因表达水平均明显低于正常组(P 0. 05);足三里组大鼠小肠黏膜组织内的SGLT1和GLUT2蛋白和基因表达水平相比于脾气虚组有所升高(P 0. 05),非经非穴组未见显著性差异(P 0. 05)。结论:电针"足三里"穴可以调节脾气虚大鼠小肠黏膜上皮组织内的SGLT1和GLUT2基因及蛋白的异常表达,参与小肠对葡萄糖的吸收作用进而改善脾气虚证。     

布地奈德雾化吸入在急性感染性喉炎患者中的应用价值

目的：研究布地奈德雾化吸入在急性感染性喉炎患者中的应用价值。方法选取82例急性感染性喉炎患者，按照随机数字法将其平均分成对照组与研究组（n=41）。对照组采用地塞米松静注，研究组采用布地奈德雾化吸入治疗，比较2组疗效。结果研究组总有效率97.6%，对照组总有效率为85.4%，2组治疗效果差异显著，有统计学意义（P＜0.05）。研究组住院时间及症状缓解时间分别为（8.6±1.7）d，（14.2±2.3）d，显著短于对照组的（15.2±2.6）d，（19.3±5.7）d，差异有统计学意义（P＜0.05）。结论采用布地奈德雾化吸入治疗急性感染性喉炎，能够显著缩短患者住院时间及症状缓解时间，值得临床推广应用。     

TQA数据趋势与TomoTherapy输出稳定性的联系

目的:探讨TomoTherapy QualityAssurance（TQA）数据趋势与螺旋断层放疗（Helical Tomotherapy，HT）系统输出的 联系。方法:回顾性分析了本院HT系统近3年内TQA各个模块的参数和数据趋势，探讨其与HT系统的静态输出剂量和 输出能量（D20/D10）变化的相关性。结果:楔形阶梯静态模块的z轴偏移参数与HT的静态输出剂量的相关性最强（r=0.883, P<0.01）。基本剂量测定模块的出口检测器平整度值对能量变化最敏感（r=0.902），其次是楔形阶梯静态模块的能量差异 （r=0.897）和楔形阶梯螺旋模块的能量差异（r=0.852），灵敏度分别为2.3×10-4、3.1×10-4和5.7×10-4。结论:TQA有助于用户 追踪HT输出剂量和能量变化，及早进行必要的机器维护或剂量校准。     

All roads lead to Rome-a review of the potential mechanisms by which exerkines exhibit neuroprotective effects in Alzheimer’s disease

Age-related neurodegenerative disorders such as Alzheimer’s disease(AD)have become a critical public health issue due to the significantly extended human lifespan,leading to considerable economic and social burdens.Traditional therapies for AD such as medicine and surgery remain ineffective,impractical,and expensive.Many studies have shown that a variety of bioactive substances released by physical exercise(called“exerkines”)help to maintain and improve the normal functions of the brain in terms of cognition,emotion,and psychomotor coordination.Increasing evidence suggests that exerkines may exert beneficial effects in AD as well.This review summarizes the neuroprotective effects of exerkines in AD,focusing on the underlying molecular mechanism and the dynamic expression of exerkines after physical exercise.The findings described in this review will help direct research into novel targets for the treatment of AD and develop customized exercise therapy for individuals of different ages,genders,and health conditions.     

Angiotensin-(1–7)reducesα-synuclein aggregation by enhancing autophagic activity in Parkinson's disease

Abnormal accumulation ofα-synuclein contributes to the formation of Lewy bodies in the substantia nigra,which is considered the typical pathological hallmark of Parkinson's disease.Recent research indicates that angiotensin-(1-7)plays a crucial role in several neurodegenerative disorders,including Parkinson's disease,but the underlying mechanisms remain elusive.In this study,we used intraperitoneal administration of rotenone to male Sprague-Dawley rats for 4 weeks to establish a Parkinson's disease model.We investigated whether angiotensin-(1-7)is neuroprotective in this model by continuous administration of angiotensin-(1-7)into the right substantia nigra for 4 weeks.We found that angiotensin-(1-7)infusion relieved characteristic parkinsonian behaviors and reducedα-synuclein aggregation in the substantia nigra.Primary dopaminergic neurons were extracted from newborn Sprague-Dawley rat substantia nigras and treated with rotenone,angiotensin-(1-7),and/or the Mas receptor blocker A-779 for 24 hours.After binding to the Mas receptor,angiotensin-(1-7)attenuated apoptosis andα-synuclein aggregation in rotenone-treated cells.Primary dopaminergic neurons were also treated with angiotensin-(1-7)and/or the autophagy inhibitor 3-methyladenine for 24 hours.Angiotensin-(1-7)increasedα-synuclein removal and increased the autophagy of rotenone-treated cells.We conclude that angiotensin-(1-7)reducesα-synuclein aggregation by alleviating autophagy dysfunction in Parkinson's disease.Therefore,the angiotensin-(1-7)/Mas receptor axis plays an important role in the pathogenesis of Parkinson's disease and angiotensin-(1-7)has potential therapeutic value for Parkinson's disease.All experiments were approved by the Biological Research Ethics Committee of Nanjing First Hospital(approval No.DWSY-2000932)in January 2020.     

子宫肌瘤栓塞失败的分析

目的:旨在评估子宫肌瘤栓塞(UFE)治疗的疗效并分析U FE治疗失败的相关因素。设计:回顾性研究。机构:大学教学医院。患者:233例1997年11月至2004年2月连续接受U FE治疗的患者。干预:3位介入放射学专家应用355-500-μ聚乙烯醇微粒实施了子宫肌瘤栓塞术。主要观察指标:子宫切除率、子宫肌瘤切除率及重复U FE治疗率。结果:患者平均随访时间为13个月。总计有22例(9.4%)患者在U FE治疗后又接受了手术治疗,其中16例(6.9%)行子宫切除术,6例(2.6%)行子宫肌瘤切除术,包括3例患者进行了重复的U FE治疗后仍需手术治疗。U FE与后继治疗的平均间…     

不明原因腹水患者腹腔镜探查的价值（附56例报告）

目的 总结腹腔镜探查对不明原因腹水患病因诊断的价值。方法：56例经多项影像学和实验室检查未能明确病因的患转行腹腔镜探查手术，其中腹壁活检29例，肝活检3例，脾活检1例，肠系膜及腹膜后淋巴结活检18例，肉眼观察但未取活检5例。结果：56例腹腔镜探查确诊51例，确诊率91.1%，1例中转开腹后确诊。结论：腹腔镜探查术创伤小、安全可靠，是不明原因腹水患的重要检查方法。     

神经症患者主客观生活事件的影响因素

目的研究应对方式、社会支持和压力反应等与应激有关因素对神经症患者主观、客观生活事件的影响。方法应用自编生活事件量表、压力反应问卷、特质应对问卷、领悟社会支持量表及一般情况调查表对88例神经症患者和88名健康对照进行调查和分析。结果神经症组主观事件得分[(13.295±11.845)分]高于健康组[(7.261±10.873)分](P<0.01),而神经症组客观事件得分[(10.239±9.105)分]与健康组得分[(8.376±11.213)分]差异无显著性(P>0.05)。神经症组主观事件与压力反应正相关,与家庭内支持和年龄负相关(P<0.01),客观生活事件与应激其他因素均无关;健康对照组主观事件与压力反应正相关,客观生活事件与压力反应和消极应对正相关(P<0.01)。多元逐步回归分析提示,影响神经症组主观事件的因素依次有客观事件、年龄、压力反应和家庭内支持,预测力为47.7%;但影响神经症组客观事件、健康组主观事件和客观事件的仅有相对应的主观或客观事件。结论神经症患者主观事件报告明显多于健康人群,且神经症患者主观事件受其他压力有关因素的影响较大。