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排序方式: 共有630条查询结果,搜索用时 15 毫秒
1.
本文用高锰酸钾氧化加刚果红染色方法对25例不同类型的淀粉样变性病进行观察分析。结果证明,本染色方法对不同种类的淀粉样物质(淀粉样轻链蛋白和淀粉样蛋白A)显示不同的颜色反应,从而可以作为区分淀粉样变性病类型的一种手段。本文对这种染色方法的机理进行了初步探讨。 相似文献
2.
患者女 ,2 6岁。 9个月前无诱因发热 ,尿蛋白 ( ) ,2 4h定量 2 4 4 g ,尿红细胞满视野且变形 ,血红蛋白 5 8g/L ,血肌酐 15 2 5 μmol/L ,3个月前外院肾活检示局灶坏死性肾小球肾炎 ,免疫荧光阴性。予地塞米松 30mg× 2d冲击 ,继以泼尼松 4 0mg ,1次 /d ,1个月后减量 ,3个月后停用。冲击治疗后血肌酐波动于 12 0~ 14 0 μmol/L ,尿蛋白 (± ) ,尿红细胞同前 ,但渐出现气短、夜间不能平卧 ,予对症治疗未见缓解 ,遂来我院就诊。既往体健。体检 :体温 38℃ ,血压 110 /5 5mmHg(1mmHg =0 133kPa) ;半卧位 ,贫血貌 ;右眼结膜下 2个针尖大… 相似文献
3.
病理性损伤因素对肾小管上皮细胞表型转化的影响 总被引:4,自引:0,他引:4
目的 :观察低血清、高糖、高蛋白刺激下肾小管上皮细胞向间叶性细胞的表型转化 ,探讨损伤的肾小管上皮细胞在肾小管间质纤维化中的作用。方法 :以体外培养的人近端肾小管上皮细胞为对象 ,分别用含低血清(0 .2mol·L-1小牛血清 )、高葡萄糖 (4.5 g·L-1)、高白蛋白 (15 g·L-1)的培养液培养 7d ,透射电镜检测细胞形态变化 ;免疫组化和Westernblot检测细胞表型改变 ,包括CK、Vimentin、α SMA、Ⅰ和Ⅲ型胶原 ,原位杂交检测Ⅰ型胶原mRNA表达 ,同时检测细胞TGFβ1蛋白表达。 结果 :低血清、高糖、高蛋白直接刺激下 ,肾小管上皮细胞出现明显形态学改变 ,包括细胞变为长梭形 ;透射电镜下细胞内线粒体明显减少 ,粗面内质网明显增加 ,并出现actin样微丝。免疫组化染色和Westernblot显示CK表达明显减弱 ,Vimentin、Ⅰ、Ⅲ型胶原、α SMA和TGFβ1表达增强。原位杂交显示Ⅰ型胶原mRNA表达增强。结论 :低血清、高糖、高蛋白可直接引起人近端肾小管上皮细胞TGFβ1表达增加 ,并发生向间叶性细胞的表型转化 相似文献
4.
邹万忠 《肾脏病与透析肾移植杂志》1992,(2)
近20年来,人们对肾脏疾病的病因和发生机理有了深入了解。各种损伤因子通过免疫反应、血管损伤、代谢障碍及直接损伤,使肾小球、肾小管及肾间质受到了轻重不等的伤害。尽管近年来在肾脏疾病的治疗方面发展很快,但仍有持续进行性恶化的病例,出现慢性肾功能衰竭,形成终末性肾(end- 相似文献
5.
原发性骨小细胞肿瘤的病理形态和免疫组化分析 总被引:3,自引:0,他引:3
目的:探讨免疫组化染色及病理形态在原发性骨小细胞肿瘤鉴别诊断中的意义。方法:对39例骨的小细胞肿瘤[其中尤因肉瘤/外周性原始神经外胚层肿瘤(EW/PNET瘤)22例、小细胞骨肉瘤6例、间叶性软骨肉瘤3例和恶性淋巴瘤8例],观察其病理形态并作免疫组化分析,所用抗体O13、NSE、S-100、Actin和LCA。结果:EW/PNET瘤20/22例为O13阳性。NSE:EW/PNET瘤16/22例和1例骨肉瘤阳性。7例EW/PNET瘤、1例骨肉瘤及3例间叶性软骨肉瘤均有S-100阳性。所有恶性淋巴瘤的LCA均为阳性。骨肉瘤均见骨样基质,间叶性软骨肉瘤可见分化较好的软骨岛。结论:O13的染色对于EW/PNET瘤有诊断意义。小细胞骨肉瘤的成骨和间叶性软骨肉瘤的软骨分化为形态上的诊断要点。 相似文献
6.
Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity 总被引:14,自引:0,他引:14
Yang WS Tsou PL Lee WJ Tseng DL Chen CL Peng CC Lee KC Chen MJ Huang CJ Tai TY Chuang LM 《Journal of molecular medicine (Berlin, Germany)》2003,81(7):428-434
Adiponectin gene polymorphisms have recently been reported to be associated with obesity, insulin sensitivity, and the risk of type 2 diabetes. We examined a T94G polymorphism of the adiponectin gene in 245 ostensibly normal nondiabetic subjects. The G allele frequency was lower among subjects with higher BMI (> or =27) than in those with lower BMI. BMI was inversely correlated with the dose of G allele. Multivariate linear regression analyses showed that the adiponectin genotypes were significantly related to BMI after adjusting for age and gender. The dose of the G allele was associated with a reduction of approximately 1.12 kg/m(2) in BMI. We further found that the relative mRNA levels of G allele were consistently higher than those of T allele in the omental adipose tissue from 21 heterozygous subjects. Finally, we observed that the expression levels of adiponectin affected insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. In conclusion, the allele-specific differential expression of this common polymorphism could be responsible for its biological effects observed in this and the other studies. 相似文献
7.
Tsou K Lowitz KA Hohmann AG Martin WJ Hathaway CB Bereiter DA Walker JM 《Neuroscience》1996,70(3):791-798
In rats, cannabinoids inhibit behavioral responses to noxious stimulation with a potency and efficacy similar to that of morphine. However, because cannabinoids depress motor function, it has not been possible to state beyond any doubt that these effects were related to a dampening of noxious sensory input. Therefore, c-fos immunocytochemistry was used to explore the possibility that cannabinoids reduce behavioral responses to noxious stimuli by decreasing spinal processing of nociceptive inputs. Rats received systemic injections of the potent and selective cannabinoid agonist WIN 55,212-2, the receptor-inactive enantiomer WIN 55,212-3 or vehicle prior to observations in a model of tonic pain, the formalin test. As demonstrated previously, plantar injections of formalin led to lifting and licking of the injected paw, with two peaks of activity occurring at 5 and 30 min after injection. The cannabinoid agonist suppressed these pain responses and produced a reduction in mobility. Immunocytochemical processing of sections with an antibody to the Fos protein revealed that the cannabinoid markedly suppressed pain-evoked c-fos expression in the superficial and neck regions of the spinal dorsal horn, but not in the nucleus proprius. Decreased expression of c-fos also occurred in the ventral horn. The specificity of this effect and its probable mediation by cannabinoid receptors are suggested by three findings: (i) the suppression by the drug of both behavioral and immunocytochemical responses to pain was dose-dependent; (ii) neither the behavioral nor the immunocytochemical response to the noxious stimulus was significantly affected by the receptor-inactive enantiomer of the agonist; (iii) animals rendered tolerant to cannabinoids by repeated injections of the agonist showed reduced responses to the drug. These findings suggest that cannabinoids inhibit the spinal processing of nociceptive stimuli and support the notion that endogenous cannabinoids may act naturally to modify pain trnasmission within the central nervous system. 相似文献
8.
病历摘要患者女性 ,74岁 ,因间断发热 1 5d ,于 1 999年 7月 9日入北京大学第一医院诊治。患者于入院 1 5d前无诱因发热 ,体温最高达 38.2℃ ,不伴寒战 ,无头痛、头晕 ,伴有纳差、恶心 ,但无腹痛、腹泻 ,未予特殊治疗。 1d前就诊于我院门诊 ,实验室检查 :血白细胞 1 3.7× 1 0 9/L ,血红蛋白 99g/L ,血小板 430× 1 0 9/L ;血沉 72mm/h ;血肌酐 437μmol/L ;尿常规蛋白 ,尿糖 ,尿沉渣镜检白细胞 2~ 3/HP。X线胸片示双肺纹理重。在院外自服羟氨苄青霉素和甲硝唑 ,病情无好转。患者既往高血压病史 30年 ,血压最高达… 相似文献
9.
病理学教学从传统模式到网络课程 总被引:2,自引:0,他引:2
病理学的教学经历了传统的教学模式、电化教学、多媒体计算机辅助教学等阶段发展到网络课程 ,教学质量和教学效率不断提高。网络课程在充分发挥多媒体和网络技术的优势的同时 ,又注意到改造传统病理学教学 (大课讲授和实验室实习 )的形式 ,使之具有现代化的内涵 ,以适应信息化社会的需要 相似文献
10.
Clinical application of NMP22 and urinary cytology in patients with hematuria or a history of urothelial carcinoma 总被引:3,自引:2,他引:1
For evaluation of the clinical application of immunoassay for nuclear matrix protein 22 (NMP22 immunoassay) and urinary cytology
for early diagnosis and detection of bladder cancer in patients with hematuria and/or a previous history of bladder cancer,
209 urine samples obtained from 137 patients presenting episodes of hematuria or a history of bladder cancer were assayed
for NMP22 levels and/or prepared for cytology examination. Biopsy was performed when any visible tumor was identified during
cystoscopy examination. The median NMP22 concentrations measured in samples taken from patients with active bladder cancer,
from patients with a history of bladder cancer but no active disease, from patients with hematuria, and from healthy volunteers
were 18.95, 5.45, 6.39, and 3.75 U/ml, respectively. The urinary NMP22 level recorded for patients with urothelial carcinoma
was significantly higher than that noted for individuals without active disease. The sensitivity of the NMP22 assay and of
urinary cytology in diagnosing bladder cancer was 69% and 67%, respectively. In contrast, the specificity of these two diagnostic
modalities reached 72% and 93%, respectively. The NMP22 assay is slightly more sensitive but less specific than urinary cytology
in detecting bladder cancer. This study indicates that determination of urinary NMP22 levels is a useful and noninvasive tool
for the detection of bladder cancer because of its high sensitivity. The urinary NMP22 assay may be used as a first-line routine
screening method; however, it cannot replace the use of urinary cytology because of its lower specificity. 相似文献