胶原海绵复合新生大鼠原代心肌细胞构建工程化心肌组织

目的:探索以胶原海绵为支架、新生大鼠原代心肌细胞为种子细胞,于体外构建工程化心肌组织的方法。方法:实验于2005-12/2006-11在解放军第四军医大学西京医院心内科实验室完成。Ⅰ型胶原海绵剪切成方形片状(2.0cm×1.4cm×0.2cm),经60Co照射消毒,于DMEM培养液中水化1h左右。另取1d龄SD大鼠心脏,剪成小碎块,然后用2.5g/L胰蛋白酶于37℃中消化,吸取上清至含胎牛血清的DMEM中,重复消化四五次,用差速贴壁法除去大部分成纤维细胞,将细胞沉淀用DMEM培养液以2×109L-1的密度悬浮备用。将上述的心肌细胞悬液1mL缓慢滴注于玻璃模型中的胶原海绵上,然后置于细胞培养中培养。肉眼及显微镜主要观察工程化心肌组织在培养期间的自发收缩情况,包括收缩的部位、强度、频率、一致性以及收缩随时间变化的情况。苏木精-伊红染色观察工程化心肌组织内胶原纤维的变化,细胞形态,胞核的形状及细胞之间的连接。免疫组织化学染色和透射电镜观察工程化心肌组织片的形态和功能。结果:①细胞接种于胶原海绵上1d后,细胞/胶原复合物的凝胶化过程基本完毕,体积保持恒定,维持至培养结束,第3天细胞/胶原复合物局部出现点片状自发收缩,第5天整个细胞/胶原复合物出现同步化自发收缩,收缩频率61～199次/min。2周后37.5%的工程化心肌组织的自发收缩活动减弱,但75%的工程化心肌组织的自发收缩活动持续至培养结束。②苏木精-伊红染色、免疫组织化学染色和透射电子显微镜显示,工程化心肌组织内细胞间连接广泛存在,细胞多呈纵向分布,胞核呈长圆形,胞浆内α-肌节肌动蛋白阳性,胞内肌原纤维排列整齐,可见到心肌特异性的肌小节结构和Z线,多数细胞具有分化的心肌细胞表型。结论:用新生大鼠原代心肌细胞为种子细胞、以Ⅰ型胶原海绵为支架材料,构建出的工程化心肌组织,于体外可长时间持续自发收缩,该细胞/胶原复合物的形态结构与生理功能均类似于成熟大鼠心肌组织。     

High expression levels of IKK alpha and IKK beta are necessary for the malignant properties of liver cancer

IKK-NF-kappa B signaling is regarded as an important factor in hepatocarcinogenesis and a potential target for liver cancer therapy.Therefore,in this study,we analyzed the expression of mRNAs encoding components and targets of NF-kappa B signaling including IKK alpha,IKK beta,RANK,RANKL,OPG,CyclinD3,mammary serine protease inhibitor(Maspin),CyclinD1,c-FLIP,Bcl-x1,Stat3,Cip1 and Cip2 by real-time PCR in 40 patients with liver cancer.After statistical analysis,7 indices including IKK alpha,IKK beta,RANK,Maspi...     

人工神经元网络用于复方氯丙嗪的含量测定

将误差反向传播(BP)的人工神经元网络(ANN)模型，用于复方氯丙嗪紫外重叠光谱分 析。对ANN模型的参数进行了优化。采用f(x)二1/(1+e^-x)作为网络节点的输入输出转换函数的三 层神经网络具有较佳性能，当取隐含节点数为15时，该网络预测结果的最小平均相对误差为1.22％， 将研制的ANN模型用于复方氯丙嗪含量测定，其结果与药品标准法和偏最小二乘法(PLs)一致。     

Elevation of cerebrospinal fluid soluble CD27 levels in patients with meningeal localization of lymphoid malignancies [published erratum appears in Blood 1996 Oct 1;88(7):2818]

Diagnosis of meningeal localization of lymphoid malignancies by means of cytologic examination of the cerebrospinal fluid (CSF) can be difficult. Thus far no reliable CSF tumor markers have been identified. CD27 is a transmembrane disulfide-linked 55-kD homodimer present on most peripheral blood T cells and on a subset of B cells. CD27 is also expressed on human malignant B cells and high levels of soluble CD27 can be present in the serum of patients with B-cell malignancies. The aim of this study is to determine prospectively the diagnostic value of CSF sCD27 as a tumor marker in patients with meningeal localization of lymphoid malignancies. CSF sCD27 levels were determined by sandwich enzyme-linked immunosorbent assay. The optimal cut-off value using receiver operator characteristics curves was found to be 10 U/mL. sCD27 levels were normal in all 50 control patients (lumbar disc protrusion) and in 39 of 40 samples obtained from patients with either solid tumors or acute myeloid leukemia. Of 104 CSF samples from 70 children with acute lymphoblastic leukemia (ALL) or non-Hodgkin's lymphoma (NHL) undergoing routine central nervous system (CNS) staging, sCD27 was false positive and false negative in only one sample each. In 70 samples from 45 patients suspected of meningeal localization of ALL or NHL, the sCD27 test had an excellent sensitivity (100%) and specificity (82%). In 7 patients with positive CSF studied longitudinally, sCD27 levels correlated very well with remission and relapse. sCD27 levels were not nonspecifically increased by the administration of cytostatic drugs. Finally, sCD27 was also elevated in the 4 patients studied with primary central nervous system lymphoma (PCNSL). CSF sCD27 is a promising tumor marker in patients with either meningeal localization of lymphoid malignancies or PCNSL, and can be useful in the differential diagnosis of CNS involvement by either lymphoid malignancies or solid tumors.     

卡尔曼滤波滴定分析法:苯扎溴铵的测定

研究了卡尔曼滤波及平滑在滴定分析中的应用,该法对热力学平衡及非平衡(动力学)体系均能适应;特别是对非平衡滴定,当许多其它方法误差较大或无法使用时,卡尔曼滤波法仍可获得正确的结果。已用于苯扎溴铵的硝酸银电位滴定分析与快速测定。     

飞行员压力性眩晕1例

０　引言　眩晕是一种旋转错觉．压力性眩晕又称气压变化性眩晕,是一种较少见的飞行错觉．在我军,有关飞行员压力性眩晕的病例尚未见报道．１　病例报告　男,３６岁,某航校歼击机飞行教员,飞行时间１５００ｈ．在飞行中曾先后两次发生滚转错觉．第１次,１９９７－０...     

可变误差多面体法用于多种维生素的同时测定

本文基于对多元校正分析模型的简要讨论,探索了应用可变误差多面体法同时测定维生索B₁,B₂,B₆和烟酰胺的可行性。其结果准确度和精密度均较满意。维生素B₁,B₂,B₆及烟酰胺的回收率分别是99.8±0.9%(CV),100.1±0.8%(CV),100.2±2.1%,100.1±0.7%(CV)。结果表明,通过公式K_S=A_SC_S^T(C_SC_S^T)^-1计算校正系数矩阵K_S,并结合可变误差多面体法这一直接求解方法,能有效地提高分析结果的准确度,克服组分间的交互作用及病态,是多元校正分析的较佳策略之一。     

同步荧光—多波长数据线性回归法测定苯酚与间苯二酚

本文提出了基于同步荧光技术的多波长数据线性回归法测定苯酚与间苯二酚,研究了荧光法测定苯酚及间苯二酚的实验条件,考察了溶液酸度、放置时间等对荧光强度的影响。方法用于复方雷琐辛涂剂中苯酚与间苯二酚的同时测定,平均回收率分别为:苯酚,97.18(±2.42)%,间苯二酚,98.15(±2.70)%。     

树突状细胞在慢性乙型肝炎和肝癌治疗中的应用前景

目的:综述树突状细胞在慢性乙型肝炎和肝癌治疗中的应用前景。资料来源:应用计算机检索PUBMED2001-01/2005-12期间的相关文章,检索词为"dendritic cells,chronic type B hepatitis,hepatoma,therapy",并限定文章语言种类为English。同时计算机检索万方数据库2001-01/2005-12期间的相关文章,检索词为"树突状细胞,慢性乙肝,肝癌,治疗",并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与树突状细胞在慢性乙型肝炎和肝癌治疗中的应用相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到56篇相关文献,30篇文献符合纳入标准,排除的26篇文献为内容陈旧或重复。符合纳入标准的30篇文献中,15篇涉及树突状细胞与慢性乙型肝炎的治疗,10篇涉及树突状细胞与肝癌的治疗,5篇涉及存在的问题与展望。资料综合:慢性乙型肝炎和肝癌的发生发展与病毒的持续感染和机体的免疫功能障碍有关,树突状细胞是机体中功能最强的抗原提呈细胞,在诱导T细胞抗病毒和抗肿瘤免疫中起核心作用。①树突状细胞与慢性乙型肝炎的治疗:许多证据表明由树突状细胞介导的细胞毒T淋巴细胞反应,通过杀伤感染的肝细胞和释放抗病毒的细胞因子来清除乙型肝炎病毒。②树突状细胞与肝癌的治疗:研究证明,经肿瘤抗原致敏的树突状细胞能明显增强特异性细胞毒T淋巴细胞对肝癌细胞的杀伤活性。结论:树突状细胞与慢性乙型肝炎和肝癌的发生发展密切相关。在乙型肝炎和肝癌治疗中,如能激发、增强机体对乙型肝炎病毒和肝癌细胞的主动免疫排斥反应,可有助于清除体内的病毒和肿瘤,达到抗病毒和抗肿瘤治疗的目的。