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排序方式: 共有74条查询结果,搜索用时 15 毫秒
1.
Vanaporn Wuthiekanun Varunee Desakorn Gumphol Wongsuvan Premjit Amornchai Allen C. Cheng Bina Maharjan Direk Limmathurotsakul Wirongrong Chierakul Nicholas J. White Nicholas P. J. Day Sharon J. Peacock 《Clinical and Vaccine Immunology : CVI》2005,12(4):555-556
An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively. 相似文献
2.
Comparison of Ashdown's medium, Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei 下载免费PDF全文
Peacock SJ Chieng G Cheng AC Dance DA Amornchai P Wongsuvan G Teerawattanasook N Chierakul W Day NP Wuthiekanun V 《Journal of clinical microbiology》2005,43(10):5359-5361
Ashdown's medium, Burkholderia pseudomallei selective agar (BPSA), and a commercial Burkholderia cepacia medium were compared for their abilities to grow B. pseudomallei from 155 clinical specimens that proved positive for this organism. The sensitivity of each was equivalent; the selectivity of BPSA was lower than that of Ashdown's or B. cepacia medium. 相似文献
3.
Allogenic human serum,a clinical grade serum supplement for promoting human periodontal ligament stem cell expansion 下载免费PDF全文
Premjit Arpornmaeklong Chotika Sutthitrairong Piyathida Jantaramanant Prisana Pripatnanont 《Journal of tissue engineering and regenerative medicine》2018,12(1):142-152
Exposing human periodontal ligament stem cells (hPDLSCs) to animal proteins during cell expansion would compromise quality and safety of the hPDLSCs for clinical applications. The current study aimed to evaluate the replacement of animal‐based serum by human serum for the expansion of hPDLSCs. hPDLSCs were cultured in culture media supplemented with four types of serums: Group A: fetal bovine serum (FBS); Group B: allogeneic human male AB serum (HS); Group C: in‐house autologous (Auto‐HS); and Group D: in‐house allogeneic human serums (Allo‐HS). Exhibitions of mesenchymal stem cell characteristics of hPDLSCs were examined. Then, growth and osteogenic (OS) differentiation potential of hPDLSCs in FBS and HS at passages 5 and 15 were compared to investigate the effects of serum supplements on growth and expansion stability of the expanded hPDLSCs. After that, growth and OS differentiation of hPDLSCs in Auto‐ and Allo‐HS were investigated. Flow cytometrical analyses, functional differentiations, cell growth kinetic, cytogenetic analysis, alkaline phosphatase and calcium content assays, and oil red O and von Kossa staining were performed. Results showed that at passage 5, HS promoted growth and OS differentiation of hPDLSCs and extensive cell expansion, decreased growth and differentiation potential of the expanded hPDLSCs, particularly in HS. Growth and OS differentiation of hPDLSCs in Auto‐HS and Allo‐HS were not different. In summary, allogeneic human serum could be a replacement to FBS for hPDLSC expansion. In vitro cell expansion of hPDLSCs should be minimal to ensure optimal cell quality. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
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5.
Monappa A Naik Premjit Sujir Sujit Kumar Tripathy Sandeep Vijayan Shamsi Hamee Sharath K Rao 《中华创伤杂志(英文版)》2013,16(2):113-117
Femoral neck stress fractures (FNSFs) are rare,constituting only 5% of all stress fractures in young adults.These fractures are usually seen in athletes,military recruits and patients with underlying m... 相似文献
6.
Direk Limmathurotsakul Matthew T. G. Holden Paul Coupland Erin P. Price Narisara Chantratita Vanaporn Wuthiekanun Premjit Amornchai Julian Parkhill Sharon J. Peacock 《Journal of clinical microbiology》2014,52(9):3418-3421
We used whole-genome sequencing to evaluate 69 independent colonies of Burkholderia pseudomallei isolated from seven body sites of a patient with acute disseminated melioidosis. Fourteen closely related genotypes were found, providing evidence for the rapid in vivo diversification of B. pseudomallei after inoculation and systemic spread. 相似文献
7.
Shimada Y Nishimura M Kakinuma S Ogiu T Fujimoto H Kubo A Nagai J Kobayash K Tano K Yoshinaga S Bhakat KK 《International journal of radiation biology》2003,79(6):423-430
PURPOSE: Ras activation is one of the major mechanisms for the development of murine thymic lymphomas by radiation and chemical carcinogens. To gain insight into the relationship between genetic susceptibility and ras gene mutation, the frequency and spectrum of ras gene mutation was examined in thymic lymphomas from susceptible and resistant mice. MATERIALS AND METHODS: K- and N-ras mutations in thymic lymphomas that arose in X-ray-irradiated and N-ethyl-N-nitrosourea (ENU)-treated mice of susceptible C57BL/6, rather resistant C3H and their hybrid B6C3F1 were analysed by polymerase chain reaction-single-strand conformation polymorphism and subsequent DNA sequencing. RESULTS: C57BL/6 exhibited a higher incidence of thymic lymphomas after exposure to X-rays and ENU than C3H, with B6C3F1 being intermediate. K-ras gene mutations occurred frequently in the pathogenesis of ENU-induced thymic lymphomas in susceptible C57BL/6 as opposed to resistant C3H. The ras mutations were more frequent in ENU-induced thymic lymphomas than X-ray-induced thymic lymphomas, and with the latter, there was no clear evidence for strain differences, suggesting that the genetic susceptibility to X-rays was independent of ras activation. The mutations of K-ras in thymic lymphomas from C57BL/6 were predominantly GGT to GAT in codon 12, whereas this mutation type was never found in those from C3H. No strain difference was observed in the nucleotide sequence or expression levels of O(6)-alkylguanine alkyltransferase, indicating that this enzyme did not account for the genetic susceptibility to ras activation. CONCLUSIONS: The results indicate that there is a clear strain and carcinogen dependency of K-ras mutation and that the frequency of ras mutation might determine the genetic susceptibility to ENU-induced lymphomagenesis, whereas pathways independent of ras activation might determine the susceptibility to X-ray-induced lymphomagenesis. 相似文献
8.
Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test 下载免费PDF全文
Amornchai P Chierakul W Wuthiekanun V Mahakhunkijcharoen Y Phetsouvanh R Currie BJ Newton PN van Vinh Chau N Wongratanacheewin S Day NP Peacock SJ 《Journal of clinical microbiology》2007,45(11):3774-3776
In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli. 相似文献
9.
Vanaporn Wuthiekanun Premjit Amornchai Daniel H. Paris Sayan Langla Janjira Thaipadunpanit Wirongrong Chierakul Lee D. Smythe Nicholas J. White Nicholas P. J. Day Direk Limmathurotsakul Sharon J. Peacock 《Antimicrobial agents and chemotherapy》2013,57(1):297-302
Pathogenic Leptospira spp., the causative agents of leptospirosis, are slow-growing Gram-negative spirochetes. Isolation of Leptospira from clinical samples and testing of antimicrobial susceptibility are difficult and time-consuming. Here, we describe the development of a new solid medium that facilitates more-rapid growth of Leptospira spp. and the use of this medium to evaluate the Etest''s performance in determining antimicrobial MICs to drugs in common use for leptospirosis. The medium was developed by evaluating the effects of numerous factors on the growth rate of Leptospira interrogans strain NR-20157. These included the type of base agar, the concentration of rabbit serum (RS), and the concentration and duration of CO2 incubation during the initial period of culture. The highest growth rate of NR-20157 was achieved using a Noble agar base supplemented with 10% RS (named LVW agar), with an initial incubation at 30°C in 5% CO2 for 2 days prior to continuous culture in air at 30°C. These conditions were used to develop the Etest for three species, L. interrogans (NR-20161), L. kirschnerii (NR-20327), and L. borgpetersenii (NR-20151). The MICs were read on day 7 for all samples. The Etest was then performed on 109 isolates of pathogenic Leptospira spp. The MIC90 values for penicillin G, doxycycline, cefotaxime, ceftriaxone, and chloramphenicol were 0.64 units/ml and 0.19, 0.047, 0.5, and 2 μg/ml, respectively. The use of LVW agar, which enables rapid growth, isolation of single colonies, and simple antimicrobial susceptibility testing for Leptospira spp., provides an opportunity for new areas of fundamental and applied research. 相似文献
10.
Babu Noushad Yeshwant Saoji Premjit Bhakat Jyothi Thomas 《The Australasian medical journal》2012,5(3):168-174