The effect of the leptin and its receptor expression in CRF rat by the Reinforcing Kidney and Exhausting Toxinthe Mixture

Objective :To investigate the adjusting malnutrition mechanism by the Reinforcing Kidney and Exhausting Toxic Mixture(REM) on the chronic renal failure (CRF) rat. Methods :60 wistar rats weved ivided into 3 groups randomly :the normal controc group(group Ⅰ ), CRF group(group Ⅱ ), and CRF rat perfusing with REM group(group Ⅲ ). Taking their fat, kidney tissue for detecting the protein expression of the leptin, leptin receptor(Ob - R) by the means of immunohistochemistry. Result : Comparing with control group, the leptin protein express intensely in CRF rat ; In kidney tissue, the ob - R express weakly. After perfusing the REM , comparing with CRF group the renal ob - R express strongly than CRF group. Conclusion : Maybe the REM could do a little better with the malnutrition of CRF rats by adjustting the activity of ob- R in kidney.     

应用聚合酶链反应快速诊断巨细胞病毒感染

用聚合酶链反应（ＰＣＲ）技术检测学龄前儿童尿标本中的巨细胞病毒（ＨＣＭＶ），并和常规细胞培养（ＣＣＣ）、早期抗原检测（ＤＥＡ）两法进行了比较。结果表明，１０５份尿标本中有４１份ＣＣＣ出现ＨＣＭＶ特征性细胞病变（ＣＰＥ），其中３８份标本ＰＣＲ检测为阳性，敏感性为９２．７％；其余６４份ＣＣＣ阴性，有６份ＰＣＲ检测阳性，特异性为９０．６％。与ＤＥＡ相比，ＰＣＲ检测的特异性和敏感性分别为９３．６％，９５．２％；提示ＰＣＲ检测临床标本中ＨＣＭＶ，不仅敏感特异，而且与ＣＣＣ，ＤＥＡ两法相比快速简便。