1. In China, Fructus Gardeniae was used as a traditional Chinese medicine (TCM) with a wide array of biological activities. The bioactive components identified in Fructus Gardeniae mainly included iridoids, flavonids, pigments, and so on. Among them, iridoids were regarded as important compounds in Fructus Gardeniae. Though analyses of the constituents in biological samples after oral administration of Fructus Gardeniae effective fraction or its active compounds have been reported, few efforts have been made to investigate the metabolic profile of Fructus Gardeniae in humans. In this study, the constituents and metabolites of Fructus Gardeniae in human blood and urine after oral administration of Fructus Gardeniae were investigated using ultra high-performance liquid chromatography (UHPLC) coupled with high-resolution LTQ-Orbitrap mass spectrometery.2. Totally, 14 constituents (two parent compounds and 12 metabolites) of Fructus Gardeniae were identified in human plasma and urine either by comparing the retention time and mass spectrometry data with that of reference compounds or by the accurate high-resolution MS/MS data of the chemicals. The compounds identified were mainly iridoid glycosides such as geniposide and the derivatives of genipin-O-glucuronide. Among them, 11 metabolites were detected in human plasma and urine while the other three metabolites including geniposidic acid (M1), demethylation derivative of genipin-O-glucuronide (M2), and dehydration product of mono-hydroxylated genipin-O-glucuronide (M9) were only discovered in human urine. Further, the possible metabolic pathways of Fructus Gardeniae in vivo were proposed and the peak area–time curve of the most abundant metabolite genipin-O-glucuronide (M13) in human plasma after oral administration of Fructus Gardeniae was depicted. The results suggested that a metabolic difference existed between rats and humans.3. The results obtained in the present research would provide basic information to understand the metabolic profile of Fructus Gardeniae in humans and explore the chemicals responsible for the hepatotoxicity of Fructus Gardeniae in vivo. Moreover, it would be beneficial for us to further study the pharmacokinetic behavior of Fructus Gardeniae in humans systematically. 相似文献
Objective: Scientific publication is the reflection of the capability of not only an individual scholar, but also a group, even a country. Over the past few decades, Chinese researchers have made great progress in medical scientific field. However, the status about the quantity and quality of the publications in otorhinolaryngology have not been reported. The aim of this study was to compare the output by Chinese authors from three regions of China: Mainland China (MC), Taiwan (TW) and Hong Kong (HK).
Methods: Literature was retrieved from the 43 otorhinolaryngology journals based on the subject category ‘otorhinolaryngology’ of the Science Citation Index Expanded (2015) from Web of Science Core Collection. The first authors of these articles were limited in three regions of China: MC, TW and HK from 2006 to 2016 by using the data mining software Thomson Data Analyzer (TDA). Evaluation criteria are based on total number of articles, impact factors (IFs), citations, articles published in high-impact journals and funding support.
Result: A total of 59,832 articles were published worldwide in 43 otorhinolaryngology related journals from 2006 to 2016. Publications from MC was rapidly increasing and the total number contributed the most articles of the China (1931/3362, 57.44%), followed by TW (1220/3362, 36.29%) and HK (211/3362, 6.28%). The quantity of annual publications from MC has exceeded that of TW since 2010. MC was in the first place for cumulative IFs, but the last place for average IF. For total and average citations, MC was in the same situation of IF. Acta Otolaryngol was the most popular journal to choose in MC, and for TW and HK was Head & Neck.
Conclusions: The total number of otorhinolaryngology articles in China increased markedly from 2006 to 2016, especially for MC. Despite the rapid growth in the number of articles from MC, the quality was not that satisfactory. 相似文献
A new modification method for glass slides was developed and applied to make ThinPrep Pap smears, in order to increase the adhesion ability of cervical exfoliative cells. 3-glycidyloxypropyl trimethoxysilane (GOPS) was coated on the glass slides firstly on the slides, then poly-L-lysine (PLL) was covalently modified onto the above epoxy-terminated slides to form GOPS-PLL double decorated slides. The modified slides were characterized using X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cell adhesion ability effect was tested and compared with traditional PLL coated slides by fixing the cervical exfoliative cells on the double adorned slides. The control test was conducted by the bare glass slides unmodified. The cell morphology of cervical exfoliative cells adhered on different slides was observed under the microscope after Papanicolaou staining. The number of cervical exfoliative cells on the unmodified slides, PLL coated slides and GOPS-PLL coated slides was 1030±300, 3283±226 and 4119±280 (n=12), respectively. The data among the three different modification methods showed significant differences (one-way analysis of variance, ANOVA test, P<0.05). The cell capturing effect of the GOPS-PLL slide was the best among the three different modified slides. In addition, the GOPS-PLL slide could enhance the uniformity of the adhered cells and be widely applied to the ThinPrep system for cervical carcinoma screening to increase the accuracy rate of diagnosis. 相似文献