Detection of Sperm DNA Damage in Workers Exposed to Benzene by Modified Single Cell Gel Electrophoresis

It was well known that the genotoxicity and the carcinogenic effects of benzene weredemonstrated[1,2]. Recently more attention had been paid to the effects of benzene on humanreproduction. These studies focused on the female reproductive system[3] or the semen,such as volume, viscosity and liquefaction[4]. Few articles reported investigating the effectsof benzene on sperm DNA that was crucial for human reproduction[5].The purpose of this study was to assess sperm DNA damage in workers expos…     

Clinical observation on 271 cases of non-Small cell lung cancer treated with yifei kangliu yin

Objective  

To observe the effects of Yifei Kangliu Yin(YFKLY) in treating non-small cell lung cancer (NSCLC).     

肺减容术治疗重度慢性阻塞性肺气肿临床疗效分析

目的：探讨肺减容手术（LVRS）治疗重度慢性阻塞性肺气肿病人（COPD）术后早期肺功能及肺血流动力学的变化。方法：64例重度COPD病人行LVRS手术,双侧19例、单侧45例。术前、术后3,6个月分别测量动脉血气（PaO2、PaCO2）、心脏超声多普勒检查（CO、CA、FS、EF、PAP）、肺功能（FEV1、RV、TLC）、6分钟运动试验（6-MWD）,并对其结果进行比较分析。结果：61例患者临床症状改善,3例死亡;单侧LVRS术后3、6个月的FEV1较术前有明显提高（P〈0．01）,双侧LVRS术后各项指标改善较单侧更好（P〈0．05）,RV、TLC较术前有明显降低（P〈0．01）;术后PaO2比术前提高（P〈0．05）,PaCO2,较术前显著减低（P〈0．05）;肺血流动力学（CO、CI、EF、PAP）无明显变化（P〉0．05）。结论：LVRS切除肺靶区20％～30％治疗重度COPD有效,术后早期肺功能明显改善、PaO2提高、PaCO2降低,而对心功能、肺动脉压力无明显负影响。     

SERUM-LIPID AND LIPOPROTEIN PATTERNS OF BEIJING POPULATIONS FROM BIRTH TO SENESCENCE

The physiologic variations of serum lipids inthe residents in urban districts of Beijing from birth to senescence were studied by standardized me- thods. 15 208 were profiled for serum total choles- terol and 5 585 for high-density lipoprotein choles- terol and triglyceride. The average serum lipids of neonates were similar to those reported by other authors over the world. The characteristic sex and age differences of total and low-density lipoprotein cholesterol showed a striking similarity to those re- ported by the Lipid Research Clinic, USA, but the mean values were much lower in Beijing residents. However, Beijing boys and men had higher high- density lipoprotein cholesterol than American coun- terparts. The cholesterol and triglyceride reached top levels at age 60-80 and declined after 80. The nonagenarians had similar lipid levels as middle- aged adults. The definition of hypercholesterolemia was discussed briefly. The lipid risk level for coro- nary heart disease of Beijing populations was es- timated.     

重组人Ⅱ型肿瘤坏死因子受体－抗体融合蛋白治疗难治性类风湿关节炎的临床疗效观察

目的：探讨重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白（rhTNFR：Fc）治疗难治性类风湿关节炎的临床疗效。方法：50例难治性类风湿关节炎（refractory or resistant rheumatoid arthritis，RRA）随机分为治疗组25例和对照组25例。治疗组：益赛普25 mg，皮下注射，每周2次，疗程6个月；对照组：安慰剂25 mg，皮下注射，每周2次，疗程24周。疗效评价采用美国风湿病学会（ACR）疗效评定标准。结果：治疗组ACR20、ACR50、ACR70有效率均高于对照组（P〈0.05），治疗组24周ACR20、ACR50、ACR70的有效率高于治疗组4周（P〈0.05）；治疗组发生不良事件6例（24.0%）高于对照组3例（12.0%）。结沦：rhTNFR：Fc治疗难治性类风湿关节炎有较好的疗效。     

调脂护肝方干预高脂饲养大鼠脂肪肝的实验研究

目的:观察调脂护肝方对高脂饲食脂肪肝大鼠的干预效应,探讨其药效学机制.方法:将雄性SD大鼠64只正常喂养1周后,随机分为正常组12只,饲基础饲料,实验组52只,给予高脂饲料饲食(含胆固醇2%,猪油10%,基础饲料88%),13周末随机抽取正常组2只,高脂饲养大鼠8只,验证高脂饲养大鼠脂肪肝形成.剩余实验组44只随机分为4组即病理模型组、东宝甘泰组、羊栖菜组、调脂护肝方组,每组均为11只,在继续饲高脂饲料外,分别给予东宝甘泰,按0.5g/(kg·d)(用蒸馏水配成0.05g/mL的混悬液),羊栖菜煎剂按10mL/(kg·d)(10mL含羊栖菜生药20g),调脂护肝方按10mL/(kg·d)(由羊栖菜、丹参、制大黄、葛根、泽泻、茯苓=3:2:1:1:1:1其中10mL含羊栖菜20g),共8周.第21周末称重,处死动物,取肝脏,计算肝脂数(肝重/体重×100%)观察肝脏组织的病理学变化,抽血,并检测以下指标:血清总胆固醇(TC)、甘油三酯(TG)、血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、血清超氧化物歧化酶(SOD)、丙二醛(MDA)、空腹血糖(FBG)、空腹胰岛素(FINS)并计算胰岛素抵抗指数(HOMA-IR)、肝TG含量.结果:①与病理模型组、东宝甘泰组比较羊栖菜组与调脂护肝方组大鼠体重及肝指数均较低(二者均为P<0.01).②与病理模型组、东宝甘泰组、羊栖菜组比较,调脂护肝方组血清TC值较低(P<0.01).③与病理模型组、东宝甘泰组比较,羊栖莱组、调脂护肝方组血清、肝脏TG均降低(P<0.01)④与正常组、病理模型组、东宝甘泰组、羊栖菜组比较,调脂护肝方组HLD-C增高(P<0.01).⑤与病理模型组、东宝甘泰组、羊栖菜组比较调脂护肝方组、正常组血清ALT、AST值均较低(P<0.01).⑥与病理模型组、东宝甘泰组、羊栖菜组比较,调脂护肝方组MDA值较低(P<0.01),SOD值较高(P<0.01).⑦与病理模型组、东宝甘泰组比较,调脂护肝方组HOMA-BI值减低(P<0.01).⑧与病理模型组、东宝甘泰组比较,调脂护肝方肝脂变率较低(P<0.05),肝组织炎症活动度也较低(P<0.01).结论:调脂护肝方治疗非酒精性脂肪肝的药效机制为:①降低体重与肝指数.②调整脂质代谢:降低血清和肝脏甘油三酯、总胆固醇.③改善肝功能:降低ALT、AST.④抗脂质过氧化,提高抗氧化酶活性.⑤改善胰岛素抵抗.⑥减轻肝脂肪变性与肝组织炎症.     

Clinical study of Gushen tablet in reducing children’s nephrotic syndrome relapse

Objective: To explore the effect of Gushen tablet (GST) in reducing the relapse of children’s nephrotic syndrome and the possible mechanism of drugs used.Methods: Fifty children with primary nephrotic syndrome who had been induced and alleviated with regular glucocorticoid (GC) were randomly divided into two groups: the GST group used GST and standard middle-long term course of GC, and the control group adopted standard middle-long term course of GC and immunoinhibitory or immunomodulatory agents for treatment. The 0. 5, 1 and 2 years after the treatment the relapse episodes, time for urinary protein negative conversion after relapse, the episodes of patients infection and relapse after infection were evaluated. Before and after treatment the plasma cortisol and T lymphocyte subpopulation were determined.Results: The relapse rate of GST group: the rates after 0. 5, 1, 2 years were 20. 0%, 30. 0% and 40. 9%, and the frequent relapse rate were 0, 6. 7% and 9. 2% respectively, which were lower than those of control group (60. 0%, 70. 0%, 69. 2% and 25. 0%, 15. 0%, 15. 4% respectively); in the GST group no relapse occurred within 0. 5 year, the relapse rate after 1 and 2 years reduced by 40. 0% and 28. 3%, compared with those of the control group (allP < 0.05); during the observation period, the mean infection/every child patient was 1. 86 episodes in GST group, after infection the nephrotic relapse rate was 28. 3%, which was lower than that of the control group (2. 25 episodes, 71.1%,P < 0.05); the relapse per patient in GST group was 0. 8 episodes, time for urinary protein negative conversion was 12. 00± 8. 98 days, lower than those of control group (1. 6 episodes, 20. 75±11. 95 days,P < 0. 05); 3 months after GST treatment the plasma cortisol level normalized, and the CD4/CD8 ratio elevated (P < 0.05).Conclusion: GST could possibly reduce the relapse of children nephrosis, and the frequent relapse and relapse episodes, and the time for post-relaptic urinary protein negative conversion shortened, the plasma cortisol elevated, and the adjustment of cellular immunity disturbance promoted. The item was supported by the Science and Technology Department of Henan Province (No. 941200528)     

基于人工神经网络与人工智能的计算机系统在儿童心理障碍诊断领域的应用

背景：将人工智能和人工神经网络二者相结合应用于精神卫生领域的文献在国内外还未见报道,更未见将人工神经网络与人工智能相结合用于模拟人类医学专家大脑诊断思维模式诊断儿童心理障碍的相关报道。 目的：用计算机模拟人脑诊断思维模式,建立一套基于人工神经网络与专家系统的儿童心理障碍标准化诊断与防治的人工智能专家系统。 方法：儿童心理障碍标准化诊断与防治的人工智能专家系统涉及儿童心理学、儿童精神病学、心理测量、心理治疗、计算机科学等诸多学科,诊断系统结合了ICD-10、DSM IV及CCMD-2等诊断标准、大规模流行病学调查数据、资深精神医学专家的丰富临床经验和临床资料。临床资料来源于全国14 家医院流调及门诊收集的原始病例,共回收有效资料1 125份,用基于神经网络与专家系统相结合的方法进行智能诊断系统的编制。 结果与结论：诊断系统能诊断61种儿童心理障碍,它包括95%以上的儿童心理障碍,在诊断之后,计算机将给出一个治疗方法建议。将195例计算机系统诊断结果与资深儿童心理精神医学专家的诊断结果进行双盲比较,诊断符合率是99%,有助于年轻医生学习资深儿童心理精神医学专家丰富的临床经验,也能帮助全国各地的心理障碍患儿,更好地为儿童心理卫生事业服务。     

Expression of Dickkopf-like1 protein(Dkkl1) in mouse testis

Objective To investigate the expression and the distribution of Dickkopf-like1(Dkkl1)protein during the development of mouse testis.Methods Testes cD NA samples from BALB/c mice in different postnatal days were hybridized with mouse whole genome affymetrix chip to screen the spermatogenesisrelated genes.The characteristics of the selected genes were analyzed by various bioinformatics tools.The mR NA expression of Dkkl1 at different stages of testis development and different tissues in mouse were analyzed by RT-PCR.The protein localization of Dkkl1 in mouse testis was assesed by immunohistochemistry.Results By analyzing the gene chip signals of mouse testis aged 4 d,9 d,18 d,35 d,54 d and 6 months,Dkkl1 was identified with a differential expression in the developmental stages of testis.RT-PCR analysis showed that the expression of Dkkl1 mR NA was firstly detected on 15 d testis tissue and gradually upregulated during the testis developing to the adult stage.The Dkkl1 protein was predominantly located in spermatocytes and round spermatids in mouse testis.Conclusion The expression of Dkkl1 is gradually upregulated during the development of mouse testis and corresponds to the mouse spermatogenesis.It may play a critical role in male mammalian spermatogenesis.