双光气损伤机制的探讨:豚鼠双光气中毒后支气管—肺灌洗液中LDH活性的变化

以LDH作为细胞损伤的指标,观察双光气对豚鼠呼吸道的损伤作用。豚鼠吸入双光气的剂量为Lct 50左右,支气管—肺灌洗液LDH活性是182±27单位％,对照组是29±7单位％,两者差异显著,中毒组灌洗液中游离血红蛋白含量不升高,表明增加的LDH不来源于红细胞。肾上腺素组灌洗液LDH活性值和中毒组灌洗液LDH同工酶谱,都提示增加的LDH来自呼吸道细胞。这些结果证明双光气对豚鼠呼吸道细胞有直接损伤作用。     

光气(双光气)中毒实验治疗

目前论及光气中毒治疗的有关资料,多主张对症支持疗法,原则是安静、保温、给氧。但所用的治疗药物不尽相同,甚至还有完全相反的主张。为此,我们对某些药物的治疗效果进行了实验观察。     

肺微血栓计数条件的探讨

用油酸性肺损伤家兔22只,研究肺循环逆流灌注液中微血栓计数条件。结果表明灌注液不需要离心,但要充分摇动并立即染色。定量取样计数5次的均值,能代表样品中的微血栓值。     

Changes of Lactate Dehydrogenase Activity in Airways of Guinea-pigs after Diphosgene Poisoning

Lactate dehydrogenase(LDH),as an indicator of cell damageor death,was used to determine the diphosgene damage of respiratorytract cells.Guinea-pigs were exposed to diphosgene at concentrationswithin the LCt_(50) range and above.The content of LDH in thebronchopulmonary washings of guinea-pigs was significantly increa-sed after poisoning.There was no increase of free hemoglobincontent in the washings.It was indicated that lysed erythrocyteswere not the source of LDH in the poisoned group.The LDH iso-enzyme pattern in the poisoned group washings suggests that theincreased LDH comes from the respiratory tract cells.These resultsshow diphosgene can directly damage cells of the respiratory tract.