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目的:建立以反相高效液相色谱-荧光法测定兔血浆中顺式阿曲库铵(Cisatracurium)浓度的方法.方法:色谱柱为Agilent Hypersil ODS-C18柱(5μm,4.0mm×125mm),流动相为50mmol/L磷酸二氢钾缓冲液(pH3.0)-乙腈(50:50),荧光检测的激发波长200nm、发射波长320nm.结果:顺式阿曲库铵检测浓度在25~2 000ng/ml线性关系良好,r=0.999 2.最低检测限为25ng/ml,日内精密度RSD≤2/56%,日间精密度RSD≤3.68%;提取回收率为85.2%~86.1%(n=5),加样回收率为93.4%~98.8%.结论:本方法简便,灵敏度高,重现性好,可满足药代动力学研究的需要. 相似文献
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mpwt ~ Theed Imaging (Din), Web reveals spatial distrhaon of inf~ abation, was aurally oonSidend as a ~ tool for ndliop and industrialaPPlicationsf Rbeenily, many ~ have ho acing tO find valuable uses of the DITI in the field of medicined~7]. The cleral uses of Din mainly includeS:.TO define the extent Of a lesion for arch a diagnosis has P~ ho made;.To localize an abnoYYnal area not Previously identified, in Older to perform ~ diagnostic teSts;.TO detect early lesions before they ar… 相似文献
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