自体骨髓移植治疗股骨头无菌性坏死

Objective To investigate the effectiveness and mechanism of transplantation of autol-ogous bone marrow in treating femoral head necrosis. Methods Sixty New Zealand rabbits were randomly divided into groups A,B,and C after establishment of the models of femoral head necrosis. The left femoral head served as the control one,and the right as the experimental group. The mitoxantrone (0.1 mg/kg) with the help of DSA was injected into the femoral head of group A. One ml autologous bone marrow was injected into the femoral head of group B. In group C,mitoxantrone (0. 1 mg/kg) was injected,and 72 h later,1 ml autologous bone marrow was injected. Four months later,all rabbits were killed,and the femoral heads were removed and observed histologically and electron microscopically. Results The number of necrotic osteoclasts in groups A and B showed no significant difference ( P > 0.05 ), ande in group C, the number of necrotic femoral heads at the left and fight sides was 40. 60±4.11 and 21.33±2.16 respec-tively ( P < 0.05 ). At the experimental side of group C, the structure of majority bone cells was clear and intact,and necrosis was occasionally seen. Conclusion At the cellular level, local chemotherapy and au-tologous hematopoietic stem cell transplantation had certain effectiveness for aseptic necrosis of the femoral head.     

自体骨髓移植治疗股骨头无菌性坏死

Objective To investigate the effectiveness and mechanism of transplantation of autol-ogous bone marrow in treating femoral head necrosis. Methods Sixty New Zealand rabbits were randomly divided into groups A,B,and C after establishment of the models of femoral head necrosis. The left femoral head served as the control one,and the right as the experimental group. The mitoxantrone (0.1 mg/kg) with the help of DSA was injected into the femoral head of group A. One ml autologous bone marrow was injected into the femoral head of group B. In group C,mitoxantrone (0. 1 mg/kg) was injected,and 72 h later,1 ml autologous bone marrow was injected. Four months later,all rabbits were killed,and the femoral heads were removed and observed histologically and electron microscopically. Results The number of necrotic osteoclasts in groups A and B showed no significant difference ( P > 0.05 ), ande in group C, the number of necrotic femoral heads at the left and fight sides was 40. 60±4.11 and 21.33±2.16 respec-tively ( P < 0.05 ). At the experimental side of group C, the structure of majority bone cells was clear and intact,and necrosis was occasionally seen. Conclusion At the cellular level, local chemotherapy and au-tologous hematopoietic stem cell transplantation had certain effectiveness for aseptic necrosis of the femoral head.     

erbB4／HER4在原发性肝癌的表达研究

目的：检测第四人体表史生长因子受体HER4在原发性肝癌组织中的表达,探讨HER4过度表达与肝癌临床病理的关系。方法：采用免疫组化的方法,检测有随访资料的56例肝癌组织中HER4的表达。结果：肝癌组织中HER4的过度表达率达82．93％,肝癌中HER4的过度表达仅与淋巴结转移、门静脉癌栓及TNM分期相关。结论：erbB4是晚期肝癌生长的一个调控基因,干预HER4的过度表达可能是治疗晚期肝癌的有效方法。     

自体骨髓移植治疗股骨头无菌性坏死

目的 探讨自体骨髓移植治疗股骨头坏死的疗效及其作用机制.方法 选用成年新西兰雄兔60只,造模后随机分为A、B、C三组.左侧股骨头作为对照组,不予处理,右侧为实验组.A组用米托蒽蓖按0.1 mg/kg量在数字减影型x射线机(DSA)导视下注入右侧股骨头内;B组在DSA下直接注入自体骨髓1 ml;C组先予以化疗(方法同A组),72h后注入自体骨髓1 ml.4个月后处死所有动物,取股骨头进行组织病理学及电镜观察.结果 A、B组内左右股骨头对比破骨细胞坏死数差异无统计学意义(P>0.05),而c组左右股骨头坏死数分别为40.60±4.11、21.23± 2.16,差异有统计学意义(P<0.05).C实验组镜下见大部分骨细胞结构清晰、完整,偶见坏死.结论 局部化疗+自体骨髓移植在细胞水平上对股骨头无菌性坏死具有一定的治疗作用.     

自体骨髓移植治疗股骨头无菌性坏死

Objective To investigate the effectiveness and mechanism of transplantation of autol-ogous bone marrow in treating femoral head necrosis. Methods Sixty New Zealand rabbits were randomly divided into groups A,B,and C after establishment of the models of femoral head necrosis. The left femoral head served as the control one,and the right as the experimental group. The mitoxantrone (0.1 mg/kg) with the help of DSA was injected into the femoral head of group A. One ml autologous bone marrow was injected into the femoral head of group B. In group C,mitoxantrone (0. 1 mg/kg) was injected,and 72 h later,1 ml autologous bone marrow was injected. Four months later,all rabbits were killed,and the femoral heads were removed and observed histologically and electron microscopically. Results The number of necrotic osteoclasts in groups A and B showed no significant difference ( P > 0.05 ), ande in group C, the number of necrotic femoral heads at the left and fight sides was 40. 60±4.11 and 21.33±2.16 respec-tively ( P < 0.05 ). At the experimental side of group C, the structure of majority bone cells was clear and intact,and necrosis was occasionally seen. Conclusion At the cellular level, local chemotherapy and au-tologous hematopoietic stem cell transplantation had certain effectiveness for aseptic necrosis of the femoral head.     

复杂胆囊切除47例临床体会

单纯胆囊切除技术上多无困难。但在胆道有先天变异,或因长期反复炎症后,胆道解剖结构发生变异,操作稍有不慎,将会酿成不良后果。笔者总结1993~2004年复杂胆囊47例切除体会予以报道。1临床资料47例中男22例,女25例,年龄41~78岁,平均59.5岁。胆道变异情况:双胆囊管1例,微小胆囊6例,胆与十二指肠瘘5例,胆与胆总管瘘3例,胆与横结肠瘘1例,胆与十二指肠、横结肠复合瘘1例,胆囊壶腹膨大8例,胆囊管异常肥大4例,迷走胆囊管5例,肝内型胆囊2例,Mirizzi胆7例,胆总管成角4例。本组病例均经手术证实胆道结构存在变异。其中迷走胆囊管、肝内型胆囊和双胆囊…     

中西医结合治疗结石性急性重症胆管炎

1993-2003年我们用中西医结合的方法治疗结石性重症胆管炎19例，其中14例取得良好疗效，现报道如下。     

自体骨髓移植治疗股骨头无菌性坏死

Objective To investigate the effectiveness and mechanism of transplantation of autol-ogous bone marrow in treating femoral head necrosis. Methods Sixty New Zealand rabbits were randomly divided into groups A,B,and C after establishment of the models of femoral head necrosis. The left femoral head served as the control one,and the right as the experimental group. The mitoxantrone (0.1 mg/kg) with the help of DSA was injected into the femoral head of group A. One ml autologous bone marrow was injected into the femoral head of group B. In group C,mitoxantrone (0. 1 mg/kg) was injected,and 72 h later,1 ml autologous bone marrow was injected. Four months later,all rabbits were killed,and the femoral heads were removed and observed histologically and electron microscopically. Results The number of necrotic osteoclasts in groups A and B showed no significant difference ( P > 0.05 ), ande in group C, the number of necrotic femoral heads at the left and fight sides was 40. 60±4.11 and 21.33±2.16 respec-tively ( P < 0.05 ). At the experimental side of group C, the structure of majority bone cells was clear and intact,and necrosis was occasionally seen. Conclusion At the cellular level, local chemotherapy and au-tologous hematopoietic stem cell transplantation had certain effectiveness for aseptic necrosis of the femoral head.     

10例急性梗阻性化脓性胆管炎致应激性溃疡大出血的抢救体会

本10例急性梗阻性脓性胆管炎(acute odstructive suppturative cholangitis,Aose)，致应激性溃疡大出血，均经保守治疗获救。文内结合文献对本病的病因，发病机理，治疗情况进行讨论，作者体会深刻并予以报道。     

自体骨髓移植治疗股骨头无菌性坏死

Objective To investigate the effectiveness and mechanism of transplantation of autol-ogous bone marrow in treating femoral head necrosis. Methods Sixty New Zealand rabbits were randomly divided into groups A,B,and C after establishment of the models of femoral head necrosis. The left femoral head served as the control one,and the right as the experimental group. The mitoxantrone (0.1 mg/kg) with the help of DSA was injected into the femoral head of group A. One ml autologous bone marrow was injected into the femoral head of group B. In group C,mitoxantrone (0. 1 mg/kg) was injected,and 72 h later,1 ml autologous bone marrow was injected. Four months later,all rabbits were killed,and the femoral heads were removed and observed histologically and electron microscopically. Results The number of necrotic osteoclasts in groups A and B showed no significant difference ( P > 0.05 ), ande in group C, the number of necrotic femoral heads at the left and fight sides was 40. 60±4.11 and 21.33±2.16 respec-tively ( P < 0.05 ). At the experimental side of group C, the structure of majority bone cells was clear and intact,and necrosis was occasionally seen. Conclusion At the cellular level, local chemotherapy and au-tologous hematopoietic stem cell transplantation had certain effectiveness for aseptic necrosis of the femoral head.