ELMO1在胃癌细胞侵袭和迁移中的作用

目的:探讨吞噬和细胞运动蛋白1(ELMO1)的表达在胃癌细胞侵袭和迁移中的作用和功能。方法:用Western blot和实时荧光定量PCR实验检测5种胃癌细胞和1种人正常胃黏膜上皮细胞ELMO1的蛋白和mRNA表达水平,并筛选出ELMO1表达量高的胃癌细胞株;用细胞转染实验沉默胃癌细胞株的ELMO1;用细胞划痕实验以及Trenswell小室迁移和侵袭实验检测抑制该基因的表达对胃癌细胞侵袭和迁移能力的影响。结果:胃癌细胞中ELMO1的表达量明显高于人正常胃黏膜上皮细胞(P0.01),其中SGC7901细胞的ELMO1表达量最高;在SGC7901细胞中ELMO1-siRNA可显著沉默ELMO1的表达(P0.05);沉默ELMO1可显著降低胃癌细胞侵袭和转移的能力(P0.01)。结论:ELMO1在胃癌细胞中高表达,并可促进胃癌细胞的侵袭和迁移。     

经输尿管镜腔内两种碎石术治疗输尿管中下段结石的疗效比较

目的探讨经输尿管镜腔内钬激光碎石术和气压弹道碎石术治疗输尿管中下段结石疗效比较.方法回顾性分析患有输尿管中下段结石187例患者,其中采用钬激光碎石术治疗95例,采用气压弹道碎石术治疗92例.比较两种碎石术的术中失血量,术后并发症的发生率,平均手术时间,一期结石清除率,平均住院时间.结果经输尿管镜腔内钬激光碎石组患者平均手术时间、平均结石处理时间,一期结石清除率等指标均优于气压弹道组.结论运用钬激光碎石术治疗输尿管中下段结石手术时间短,结石清除率高,具有较高的有效性和安全性.值得临床推广.     

ELMO蛋白家族与恶性肿瘤侵袭转移关系的研究进展

It is one of the main characters of malignant tumors that malignant tumor cells invade surrounding tissues and metastasize to distant tissues. Multiple factors are involved in this complicated dynamic process. Metastasis is the major factor influencing recurrence and prognosis. Therefore, it is important to explore the mechanism of invasion and metastasis for reducing recurrence rate and mortality of malignant tumors. Engulfment and cell mobility (ELMO) family is one kind of conserved protein in evolutional process. It includes 3 members, ELMO1, ELMO2 and ELMO3. The members of ELMO family play an important role in cell phagocytosis and cell migration, and they also have close correlation with malignant tumor cell invasion and metastasis. In this paper, we review the progress of the relationship between ELMO family and malignant tumor invasion and metastasis.