内分泌和化疗对乳腺肿瘤患者干细胞的影响

BACKGROUND:Tumor stem cells are the root of cancer recurrence and metastasis, so clinical researches should focus on the effects of different treatments on tumor stem cells. OBJECTIVE:To explore the effects of endocrine therapy and chemotherapy on stem cells in patients with breast cancer. METHODS:After recovery and cultivation of estrogen receptor-positive human breast cancer cell lines MCF-7, passage 3 cells in logarithmic phase were selected and divided into three groups containing control, estradiol and estradiol with tamoxifen groups. The estradiol group was divided into three subgroups: 10^-7, 10^-8 and 10^-9 mol/L estradiol was added into the medium, respectively; the estradiol with tamoxifen group was divided into three subgroups: 10^-7, 10^-8 and 10^-9 mol/L estradiol with 10^-6 mol/L tamoxifen were added into the medium, respectively. The same amount of absolute ethyl ethanol was added into the medium of control group. Fifteen female patients with late recurrence and metastasis of breast cancer received chemotherapy as recurrence and metastasis group. Another 15 healthy volunteers were selected as healthy control group. RESULTS AND CONCLUSION:The proportion of CD44⁺CD24^-/low cell subsets in the estradiol and estradiol with tamoxifen groups was significantly higher than that of the control group (P < 0.05), and the proportion of CD44⁺CD24^-/low cell subsets in the estradiol group was significantly higher than that of the estradiol with tamoxifen group at the same concentration (P < 0.05). The proportion of CD44+CD24-/low cell subsets had no significant differences among groups at 10 and 20 days of culture (P < 0.05). The proportion of CD44⁺CD24^-/low cell subsets significantly increased in MCF-7 cells after 24-hour intervention with different chemotherapy drugs. But only the proportion of CD44⁺CD24^-/low cell subsets in the paclitaxel and doxorubicin groups was significantly higher than that of the control group after 20-day intervention (P < 0.05). Besides, the proportion of CD44⁺CD24^-/low cell subsets in the peripheral blood of healthy volunteers was significantly lower than that of the recurrence and metastasis group (P < 0.05). Among 15 patients with late recurrence and metastatic of breast cancer, 9 had stable disease, 5 had partial remission, 1 had failed chemotherapy and cancer progression. Moreover, the proportion of CD45^-CD44⁺CD24^-/low cell subsets in the peripheral blood of patients sensitive for chemotherapy was significantly lower than that before treatment (P < 0.05). In conclusion, both endocrine therapy and chemotherapy exert a certain effect on the CD44⁺CD24^-/low cell subsets of breast cancer positive for estrogen receptor. Given that CD44⁺CD24^-/low cell subsets in MCF-7 cells resist chemotherapy drugs, the proportion of CD45^-CD44⁺CD24^-/low cells in the peripheral blood of patients sensitive for chemotherapy is decreased.     

反思式基础护理学实验报告的实践与分析

目的探讨并分析在中职基础护理学实验教学中应用反思式实验报告。方法在许昌卫生学校2009级普通中专基础护理学实验教学中应用反思式实验报告,对学生的学习效果进行评价,并自行设计问卷对学生的实验报告书写情况进行调查。结果通过书写反思式实验报告,实验组学生知识和技能的掌握优于对照组,且实验组学生认为自身理论联系实际的能力得到了提高,同时,此种方法有助于增进师生互动(P<0.01)。结论反思式实验报告的书写能够提高学生的评判性思维能力,增强学生的自主学习能力,促进师生间进行有效沟通。     

不伤和气的拒绝技巧

做你没做过的事情叫成长,做你不愿意做的事情叫改变,做你不敢做的事情叫突破。随着年龄的增长,我们并不是失去了一些朋友,而是懂得了谁才是真正的朋友。同事和朋友是漫漫人生中的一种缘分,和睦相处更是互利共赢的发展根基。为了维系融洽和谐的关系,难免会遇到别人求你帮忙或邀请赴约,如果你不情愿或没有能力办到,怎么回绝呢？一、看场合,再拒绝私下拒绝,往往比当众拒绝好。有旁人在,     

206例外阴阴道假丝酵母菌病的临床疗效分析

目的:探讨硝呋太尔制霉菌素阴道软胶囊联合氟康唑片对外阴阴道假丝酵母菌病的临床疗效。方法:随机将206例患者分为对照组和实验组,对照组接受单纯的硝呋太尔制霉菌素阴道软胶囊治疗,实验组在对照组的基础上给予口服氟康唑片联合治疗,比较两组患者的临床疗效,不良反应和复发率。结果:实验组疗效优于对照组,复发率低于对照组,两组患者不良反应发生率无统计学意义。结论:硝呋太尔制霉菌素阴道软胶囊联合氟康唑片治疗,疗效显著,复发率低。