dC14可抑制小鼠SRS腹水瘤的发病和生长

作者曾经体外实验证明,均聚寡脱氧胞嘧啶核苷酸片段(dC14)有抗病毒活性,现继续研究其在体内的作用。不给dC14的对照组小鼠,接种腹水瘤细胞后:100％发病和死亡,潜伏期为6d,存活期11d;给每只小鼠dC14 1.3～3.2 mg,发病率和死亡率降低至20％,疾病潜伏期和存活期推迟。在未经dC14处理的种瘤细胞和淋巴组织与经处理的淋巴组织中,多聚酶链反应(PCR)法都检测到病毒核酸,而正常小鼠中则无此种病毒核酸顺序。研究结果提示,dC14对小鼠SRS腹水瘤的发病和生长有抑制作用。此种抑制作用可能是通过抑制病毒基因的表达来实现的。     

反义脱氧寡核苷酸对SRS病毒复制的抑制作用

用合成的反义脱氧寡核苷酸,包括修饰的和非修饰的,同聚的和异聚的脱氧寡核苷酸对SRS病毒感染细胞的增殖、细胞群落形成,XC融合细胞产生的抑制作用。结果表明,上述的寡聚物对病毒都有抑制活性。各种寡聚物的作用机制不同。     

反义寡核苷酸抑制人急性早幼粒细胞白血病细胞增殖和蛋白表达

目的 :研究反义寡核苷酸 (antisenseoligonucleotides)对人体引起急性早幼粒细胞白血病的HL 6 0细胞增殖和蛋白质表达的抑制作用。方法 :设计和合成了不同序列、长度和核苷酸磷酸根上有无修饰的多种脱氧寡核苷酸片段 ,进行了对HL 6 0细胞的生长抑制和蛋白表达的抑制试验 ,也测定了它们对人体正常淋巴细胞的毒性。结果 :发现互补于C mycmRNA的 2个区域的反义寡核苷酸序列 (5′ 4 4 6 1和 5′ 556 576 )有较好的抑制效率 ,在一定时间内 ,HL 6 0细胞增殖抑制分别达到 59.5%和6 2 .7% ,并且它们对人体正常淋巴细胞无毒性作用。结论 :我们新设计的互补于 5′ 4 4 6 1序列片段对HL 6 0细胞增殖有抑制作用 ,有望发展成抑制HL 6 0细胞增殖的核酸药物。     

dC14 CAN INHIBIT THE INCIDENCE AND GROWTH OF MOUSE SRS ASCITIC TUMOR

The antiretroviral action of dC14 in vitro has been proven in previous experiments. This paper deals with the effect of dC14 in vivo.Intraperitoneal inoculation of tumor cells into mice without dC14 treatment induced ascitic tumors after a latency of 6 days and death of the mice after 11 days. Both incidence and mortality of disease were 100%. In animals receiving dC14 treatment, the incidence and mortality were less, latency and survival periods were longer. Viral sequences could be detected in tumor cells and in spleens of tumor cell inoculated animals, receiving dC14 treatment or not but could not be detected in normal animals. Studied suggest that the suppressive action of dC14 on the development of mouse SRS ascitic tumor may be through blocking gene expression of virus.