血浆丙戊酸钠的气液色谱测定

丙戊酸钠是一种抗癫痫新药,为了能找到它的合适治疗剂量及药物代谢动力学研究,本文报导了快速测定血浆中微量丙戊酸钠的气液色谱分析法。将含有丙戊酸钠的血浆酸化后,用氯仿萃取其中游离出的丙戊酸,以每次1微升氯仿萃取液在涂渍了3?AP的Gas Chrom Q色谱柱上直接进样,以N_2为载气,氢火焰离子检测器检测,进行气液色谱分析。分析结果,丙戊酸的色谱峰面积与其相应浓度(在0.1～0.5微克/微升)为直线关系。本分析方法操作简便快速,定量准确,重现性好,能适用于临床。     

尿乳酸简易检验法

乳酸中毒没有典型的先兆症状,而可靠的早期检验,到目前为止唯一可能实行的是冗长的血乳酸盐测定法。然而血乳酸盐的浓度和排出的尿乳酸盐之间存在着相互关系。测定尿中乳酸盐可估计血中浓度。本文提出一种简易、快速的尿乳酸检验法。应用本法对急诊室病人和实验性诱发高乳酸血症病人作了检查,结果证实本法对尿乳酸增高的检为验是可靠的。     

血浆和全血乳酸的快速床边测定

本文叙述一种用于床边测定全血、血浆和细胞内乳酸的快速方法。测定用的仪器为Lactate Analyzer 640。该法特点为仅用极少量的血(100μl),而且测定可在2～3分钟内完成。Lactate Analyzer 640的原理是在6氰高铁酸盐[Fe(CN)_6]~3-和细胞色素b_2酶的存在下,乳酸转变为丙酮酸的特有的和不可逆     

N-(对硝基重氮苯)对氨基偶氮苯和N-(4-硝基重氮萘)对氨基偶氮苯用作高汞试剂

试鎘剂学名N-(对硝基重氮苯)对氨基偶氮苯(P-nitrobenzenediazoaminoazobenzene,Cadion)和试镉剂2B学名N-(4-硝基重氮萘)对氨基偶氮苯(4-nitronaphthalenediazoaminoazobenzene,Cadion 2B)是定性分析中用作检难Cd~( 2)和Mg~( 2)的一个很好试剂。Dwyer首先初步研究了这二个试剂在2N氫氧化鉀溶液中与一般阳离子的作用并推荐了     

METABOLISM PATTERN OF FECAL BILE ACIDS IN PATIENTS WITH LARGE BOWEL CANCER

The feca! bite acids were extracted from 21 patients with large bowel (colonic and rectal) cancer and 21 controls, and the bile acid composition and concentration were measured by gas chromatography. The total bile acid concentration and concentration of individual bile acids were not statistically different between colonic and rectal cancer. Bat the concentration and the percentage composition of secondary bile acids (deoxycholic and lithocholic acids) were significantly higher than that in controls. However the percentage composition of primary bile acids (cholic and chenocholic acids) were significantly lower than that in controls. The results suggest that incidence of large bowel cancer is closely related to the metabolism of fecal bile acids, and the etiology of colonic and rectal cancers may be the same.     

慢性胃炎患者胃液胆汁酸的测定意义

应用气相色谱仪对100例慢性胃炎患者进行胃液胆汁酸浓度测定,结果慢性胃炎患者胃液内胆汁酸浓度均显著高于正常人,其中尤以慢性萎缩性胃炎伴肠腺化生和不典型增生者最为明显,慢性萎缩性胃炎不伴肠腺化生者次之,慢性表浅性胃炎则增加最少。