STUDY ON ENHANCEMENT OF TUMOR-KILLING EFFECT AND FORMATION OF NEW FLUOROPHORE IN TUMOR CELLS

Hyperthermia (42-44℃) and photosensitizing therapy can destroy S180 tumor cells,reducemalignant ascites and prolong the survival times of mice with carcinomas.The highestcurative effect was observed when using a combination of the two treatments.Heating to44℃ has a greater destructive effect on tumor cells than has heating to 42℃.The resultsshow that this is due to a synergistic interaction between these two treatments.The fluores-cence spectrum of S180 cells was determined before and alter treatment,and the indicationwas that the synergistic effect is probably related to a new fluorescence product;the greaterthe intensity of the new fluorescence、the more marked the synergy of hyperthermia andphotosensitizing therapy.The maximum emission wavelength was 460nm (excitation wave-length 370nm).     

血卟啉—激光对小鼠正常皮肤生物效应的电镜观察

实验动物和条件同《光镜观察)一文。电镜观察:光敏处理后5～10分钟已可见到微血管内皮细胞肿胀和线粒体损伤,神经纤维末稍分支的轴突和雪旺氏细胞浆内出现空泡,成纤维细胞的线粒体肿胀和粗面     

血卟啉—激光对小鼠正常皮肤生物效应的光镜观察

小鼠按每公斤体重50毫克腹腔注射HpD后2天,激光照射左耳5分钟,波长630nm,输出功率250mW,光斑直径1cm。除正常对照组3只小鼠外,单纯HpD组、单纯激光组和光敏处理组小鼠均于处理后即刻至14天分批处死取两耳,每组3只(少数组2只),切片,HE染色,观察。单纯HpD组鼠耳组织无明显改变。