腕关节三角软骨板的测量及临床意义

目的通过解剖测量,了解腕关节三角软骨板主要的形态参数,为临床应用提供解剖学基础。方法通过对40例成人腕骨标本进行解剖,解剖后观察三角软骨板的大致形态,对其直径厚度及其与周围组织的距离进行测量。结果对标本进行解剖及测量后得出数据采用SPSS20．0统计分析软件分析得出结果为：腕关节三角软骨板背侧的厚度为（5．38±0．56）mm,尺侧缘厚度为（6．45±0．32）mm,桡侧缘厚度为（2．58±0．48）mm,掌测的厚度为（7．49±1．27）mm,中央最薄处厚度为（1．05±0．20）mm,掌背侧直径（17．62±3．56）mm,尺桡侧直径为（13．04±2．67）mm。结论通过解剖测量得出了三角软骨板较为完整的数据;临床应用中按照三角软骨板测量数据及解剖特点进行诊断和治疗,可能有助于减少其损伤的发生。     

兔主动脉血管内皮细胞原代改良提取法

BACKGROUND: Primary vascular endothelial cells are mostly harvested through aorta endothelial cell cultures and micro-artery endothelial cell cultures using enzyme digestion and tissue adhesion methods, and the quality and purity of harvested cells cannot meet the need for current scientific research. OBJECTIVE: To investigate an improved extraction of primary vascular endothelial cells and the relevant identification method. METHODS: A segment of rabbit aorta was cut to culture vascular endothelial cells using the improved extraction method in group A or using adhesion method in group B. In the group A, the vascular intima was striped out with microsurgical instruments, and digested enzymatically to acquire single primary cells followed by culture in endothelial cell culture medium. In the group B, the whole vascular intima was adhered to the culture dish that was incubated in a 5%CO2, 37 ℃ incubator for 1 hour. Cell pellets in the two groups were cultured in vitro. Cell morphology was observed using a microscope; immunohistochemical staining was used to detect CD31, VIII factor and Vimentin protein for identification of vascular endothelial cells. RESULTS AND CONCLUSION: The purity and number of vascular endothelial cells extracted by the improved method were higher than those by the adhesion method. Immunohistochemical findings showed positive expression of CD31 and VIII factor, but negative expression of Vimentin. These findings indicate that the improved extraction method can obtain more vascular endothelial cells with higher purity, which is of strong operability and practicality.