DETECTION OF HUMAN CYTOMEGALOVIRUS INFECTION BY DNA-DNA HYBRIDIZATION

A rapid detection method has been established to identify human cytomegalovirus (HCMV) in specimens of urine and white blood cells with use of DNA-DNA hybridization technology. Since radioactive labels should be avoided if possible, the DNA-DNA hybridization procedure has also been modified to employ biotin labelled probe. Preliminary experiments for sensitivity and specificity showed that hybridization with the probe HCMV DNA EcoRI J fragment could detect 0.9 pg (~(32)P-probe) or 90 pg (biotin-probe) homologous DNA; 60 cells (~(32)P-probe) or 325 cells (biotin-probe) of HCMV infected fibroblasts, and 0.3125 TCID_(50) HCMV-AD_(169). The labelled probe failed to hybridize to DNA from ather herpesviruses, uninfected human lung fibroblasts and human placental DNA. Some specimens have been detected for HCMV by DNA hybridization with ~(32)P-probe. Of 76 urine specimens and 46 white blood cell specimens from 51 newborn infants with clinically suspected cogenital HCMV infection, 11 (12%) and 9 (19%) were positive for HCMV DNA, respectively. Of 11 white blood cell specimens from normal women of childbearing age and 47 white blood cell specimens from blood donors, 6 (55%) and 30 (63%) were positive for HCMV DNA, respectively.     

巨细胞病毒DNA杂交试验检测新生儿巨细胞包涵体病

本文报道应用同位素~(32)p及生物素标记CMV-AD_(169)株Eco RIJ片段作探针,进行CMV-DNA斑点杂交试验,同时取清洁中段尿作病毒分离。16例新生儿检测结果尿DNA杂交试验及血白细胞DNA杂交试验均阳性6例,单独尿阳性2例,白细胞阳性4例,总计12例,检出率71%。与尿病毒分离相对照,有显著的一致性,根据本组病例检测结果认为本试验具有快速、可靠、简便、稳定、重复性好、敏感性与特异性强的诊断效果,为新生儿CID的早期诊断与防治提供了良好的检测手段。     

用小鼠腹腔巨噬细胞与同系肿瘤细胞混合培养制备免疫干扰素(Ⅱ型)

产生干扰素是生物体对各种不同的刺激如病毒抗原以及致有丝分裂物的一种反应。按诱导物和产生细胞的不同而产生不同类型的干扰素,即:Ⅰ型或α和β型,Ⅱ型(免疫)或γ型干扰素。关于恶性肿瘤细胞与免疫活性细胞相互作用能产生干扰素以前曾有     

巨细胞病毒DNA杂交试验检测新生儿巨细胞包涵体病

人巨细胞病毒(HCMV)是围产期病毒感染的最常见原因之一,可导致流产、死胎、畸形或先天性巨细胞包涵体病(CID)。欧洲国家报道孕妇感染率3～28％,感染孕妇分娩的新生儿约50％初生时可无症状,但以后逐渐出现进行性智力减退或脑积水等,15％出生即发生典型的CID。由于CMV感染常无特殊临床表现,诊断主要依靠实验室检测。常用方法有尿找病毒包涵体、病毒分离、补体结合试验,特异性IgM测定等。但以上检测方法或因敏感性和特异性低,或因所需