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目的 探讨骨髓干细胞移植和伞状支撑骨移植治疗股骨头坏死的效果.方法 取6只健康山羊,采用液氮冷冻法制作单侧股骨头坏死动物模型,8周后在坏死侧行骨髓干细胞移植和伞状支撑骨移植术.分别于术后3、6个月做放射学检查,观察股骨头内骨质变化.结果 实验动物一般状况良好.液氮冷冻法8周后达成股骨头坏死模型,模型侧后肢出现跛行.骨髓干细胞移植和伞状支撑骨移植术后3个月X线显示,股骨头外形恢复,股骨头内囊性低密度区消失,可见骨柱影,股骨头内骨质愈合状况良好,原塌陷已修复,股骨头无再塌陷.6个月时,治疗侧股骨头外形正常,骨密度基本均匀,骨柱影已模糊,植骨已融合,股骨头无再塌陷.正常对照侧股骨头无异常变化.结论 骨髓干细胞移植和伞状支撑骨移植术可以有效治疗骨坏死,防治股骨头塌陷,效果良好.
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Objective To study the usefulness to treat the osteonecrosis of the femoral head (ONFH) with bone marrow mesenchymal stem cell transplantation and umbrella strut bone grafting. Methods Six goats were established of osteonecrosis of the femoral head models on one side by method of liquid nitrogen frozen, and then they were taken into bone marrow mesenchymal stem cell transplantation and umbrella strut bone grafting after 8 weeks. The radiological examination was made at 3 and 6 months after the bong grafting, and observed changes of bone union in the femoral head. Results The general state of the experimental animal was fine after the operation. The model of ONFH was reached on 8 weeks after liquid nitrogen frozen, and limping. After 3 months of bone marrow mesenchymal stem cell transplantation and umbrella strut bone grafting, X-ray film showed that low bone density disappeared, the shape of the femoral heads, grafting strut bone and initial bone union were fine in the head, and no repeated collapse of the head. The X-ray film showed that the shape of the femoral heads was normal, grafting strut bone was union in the head, and no repeated collapse of the head after 6 months. There was normal on the control side. Conclusions The ONFH can be treated effectively by bone marrow mesenchymal stem cell transplantation and umbrella strut bone grafting, and the collapse of the femoral head is prevented. 相似文献
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目的:客观评价中医益气活血利湿清热法(以下简称“益气活血清利法”)联合西药治疗特发性膜性肾病(idiopathic membranous nephropathy,IMN)的有效性与安全性。方法:检索中文数据库:中国知网、维普、万方、中国生物医学文献,英文数据库:Pub Med、The Cochrane Library、Medline中建库至2021年10月发表的益气活血清利法联合西药治疗IMN的随机对照临床试验研究文献,参照Cochrane Collaboration标准对文献进行质量评价,并提取试验的疗效评价指标:24 h尿蛋白定量、血清白蛋白、血肌酐、尿素氮、血清总胆固醇、甘油三酯,以及安全性评价指标:不良反应发生情况。运用Review Manager 5.3软件进行统计学分析。结果:纳入符合条件的文献14篇,纳入患者836例。Meta分析结果显示,益气活血清利法联合西药治疗IMN,对降低患者24 h尿蛋白定量[WMD=-0.97,95%CI(-1.30,-0.65),Z=5.86,P<0.001]、升高血清白蛋白[WMD=3.83,95%CI(2.10,5.57),Z=4.... 相似文献
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目的 观察靶向PPARγ基因小干扰RNA(siRNA)预防灌胃法家兔乙醇性股骨头坏死的效果.方法 将96只家兔随机分为4组,每组24只.N组:灌胃法给予生理盐水10ml/(kg·d).M组:灌胃法给予烈性酒(含乙醇度46%,V/V)10 ml/(kg·d);S组:实验1、3、5个月第1天,随机选择侧别手术,穿刺注入股骨头内25 μl siRNA重组腺病毒,同M组灌酒;CO组:同S组手术,同M组灌酒,不注入siRNA重组腺病毒.于2、4、6个月末分批处死家兔,观察血清学和股骨头组织病理学变化.结果 实验6个月时,N、M、CO、S组血清甘油三酯含量分别为(1.21±0.12)、(4.59±1.58)、(4.63±1.17)、(4.32±1.20)mmol/L;总胆固醇含量分别为(2.35±0.33)、(19.59±1.58)、(20.13±1.17)、(18.32±1.20)mmol/L;脂肪细胞平均直径分别为(40.89±2.41)、(48.65±2.93)、(49.45±2.63)、(42.52±2.57)μm;骨小梁面积分数分别为(41.80±2.47)%、(30.70±2.86)%、(29.80±2.69)%、(41.60±2.87)%;空骨陷窝计数分别为(12.30±1.73)%、(22.40±1.52)%、(23.10±1.62)%、(11.70±1.46)%;PPARγ表达量分别为(0.29±0.05)、(0.66±0.12)、(0.61±0.15)、(0.33±0.05);骨钙素mRNA表达量分别为(0.92±0.07)、(0.19±0.11)、(0.23±0.08)、(0.88±0.11);PPARγ蛋白表达量分别为(0.75±0.08)、(1.60±0.11)、(1.55±0.12)、(0.65±0.05).M、CO组病理变化明显,髓内造血组织减少,脂肪细胞增殖肥大,骨小梁变细、稀疏、部分断裂;S组股骨头内最大脂肪细胞平均直径、空骨陷窝计数、骨坏死发生率、PPARγ基因与蛋白表达量均明显低于M、CO组(P<0.01,P<0.05),S组与N组差异无统计学意义(P>0.05).结论 靶向PPARγ基因siRNA腺病毒载体能够有效阻断乙醇诱导的家兔股骨头内MSCs中PPARγ基因表达及成脂分化,预防乙醇性ONFH的发生.Abstract: Objective To observe the effect of small interfering RNA (siRNA) adenovirus vectors targeting PPARγto prevent the alcohol-induced osteonecrosis of the femoral head (ONFH) in rabbits.Methods Ninety-six rabbits were randomly divided into four groups. In group N, normal saline ( 10 ml/kg every day) was poured into stomach. In group M, the strong wine (the volume fraction was 46% alcohol,10 ml/kg every day) was poured into stomach. In group S, under the randomly selected side and anesthesia, the animals were injected with 25 μl siRNA adenovirus drip into the femoral head and then the puncture hole was closed on the 1st day at the 1st, 3rd and 5th month of the experiment, and at the same time the strong wine (the volume fraction was 46% alcohol, 10 ml/kg every day) was poured into stomach. In group CO, animals were treated with the same method as the group S, but not injected with siRNA adenovirus drip. The animals were sacrificed in batches at 2nd, 4th and 6th month after the experiment. The serology and pathological changes of the femoral head were studied. Results At the 6th month, the triglyceride (TG, mmol/L) contents in groups N, M, CO and S were (1.21 ±0. 12), (4.59 ± 1.58), (4.63 ±1.17) and (4. 32 ± 1.20), the cholesterol levels ( CHO, mmol/L) were (2. 35 ± 0. 33), ( 19. 59 ±1.58), (20. 13 ± 1. 17) and ( 18.32 ± 1.20), the average diameter of the max adipocyte (μm) was (40. 89 ± 2. 41 ), (48.65 ± 2.93 ), (49. 45 ± 2. 63 ) and ( 42. 52 ± 2. 57 ), the trabeculace area fraction was (41.80 ±2. 47)%, (30. 70 ±2. 86)%, (29. 80 ±2. 69)% and (41.60 ±2. 87)%, the percentage of empty osteocyte lacunae was (12.30 ± 1.73)%, (22.40 ± 1.52)%, (23. 10 ± 1.62)% and (11.70 ±1.46)%, the expreasion levels of PPARγ mRNA were (0.29 ±0.05), (0.66±0. 12), (0.61±0.15) and (0. 33 ± 0. 05 ), the expression levels of osteocalcin mRNA were (0. 92 ± 0. 07 ), (0. 19 ± 0. 11 ), (0. 23 ±0. 08) and (0. 88 ± 0. 11 ), the expression levels of PPARγprotein were ( 0. 75 ± 0. 08 ), ( 1.60 ± 0. 11 ),(1.55 ±0. 12) and (0.65 ±0.05), respectively. In groups M and CO, the pathological changes were obvious; there was decreased hematopoietic tissue, proliferation and hypertrophy of the adipocytes, increased fatty tissue, and thinned, sparse or breoken bone trabeculae in the femoral head; the area fraction of the trabeculae was reduced. In group S, the average diameter of the max adipocyte, percentage of empty osteocyte lacunae, incidence of ONFH, the expressions of PPARγmRNA and protein were obviously reduced as compared with groups M and CO (P <0. 01,P <0. 05). There was no statistically significant difference between group S and group N (P > 0. 05). Conclusion siRNA adenovirus vectors targeting PPARγcan efficaciously suppress the expression of PPARγ gene and adipogenic differentiation of the MSCs in the femoral head induced by alcohol, which may prevent the development of the alcohol-induced ONFH in rabbits. 相似文献
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目的通过动物实验,以西药厄贝沙坦为对照,观察中药益气祛风通络方对糖尿病肾病大鼠尿蛋白、血糖、肾功能的影响,探索益气祛风通络方治疗糖尿病肾病的疗效。方法将35只Wister大鼠随机分成正常组、假手术组、模型组、中药组、西药组,通过高脂高热量喂养加腹腔注射链脲佐菌素加单侧肾切除术复制糖尿病肾病模型,检测各组大鼠24小时尿蛋白,尾尖取血测血糖,腹主动脉取血测血清肌酐。结果 (1)干预前后,各组的24小时尿蛋白差值分别是中药组(-10.80±5.02)×10~(-3 )g、西药组(-3.38±8.67)×10~(-3 )g、模型组(15.48±17.95)×10~(-3)g、假手术组(1.22±2.19)×10~(-3 )g、正常组(-0.16±1.48)×10~(-3 )g,中药组大鼠24小时尿蛋白定量明显下降,与假手术组相比P=0.007,与正常组相比P=0.015,差异都有统计学意义(P0.05)。(2)在药物干预的12周里,只有中药组的血糖在持续下降,其余各组血糖值上升,中药组干预前后血糖差值与西药组相比P=0.001,与模型组相比P=0.000,与假手术组相比P=0.001,与正常组相比P=0.000,差异均有统计学意义(P0.05)。(3)干预12周后,各组大鼠腹主动脉取血,测血清肌酐值分别是中药组(29.23±1.65)μmol/L、西药组(41.52±5.47)μmol/L、模型组(53.33±12.76)μmol/L、假手术组(31.42±2.61)μmol/L、正常组(29.18±1.37)μmol/L,中药组血肌酐值较低,与西药组相比P=0.011,与模型组相比P=0.028,差异均有统计学意义(P0.05);模型组血肌酐值较高,与假手术组相比P=0.040,与正常组相比P=0.028,差异均有统计学意义(P0.05)。结论益气祛风通络方对糖尿病肾病大鼠的24小时尿蛋白有降低作用,同时还能降低血糖、保护肾功能,疗效优于西药厄贝沙坦。 相似文献