外源怀钙对放烧复合伤早期心肌线粒体呼吸功能的影响

目的：了解外源性钙离子对放烧复合伤心肌线粒呼吸功能的影响。方法：Ｗｉｓｔａｒ大鼠分为正常对照组，６Ｇｙ照射复合３０％ＴＢＳＡⅢ度烧伤组、放烧复合伤预防用药组 簇烧复合伤治疗用药组。于伤后１、１２、２４和４８ｈ测定不同浓度钙对呼组线粒体呼吸功能的影响。结果：低浓度外源性钙对正常对照组心肌线粒体呼吸有刺激作用，但对放烧复合伤组、放烧复合伤预防用药组和治疗用药组线粒体呼吸无显著刺激作用；高浓度外源性钙（     

眼球内玻璃异物前房内移位

患儿男12岁4年前患儿玩耍时将火炮放入坏灯泡内,并点燃火炮发生爆炸,玻璃碎片当即溅入左眼.被家长发现后急送我院检查,眼眶正侧位片显示:球内约5点钟处有3×1mm条状异物影.     

Abnormal calcium transport ofmyocardial mitochondria during the early stage of combined radiation－burn injury in rats

Ａｂｎｏｒｍａｌｃａｌｃｉｕｍｔｒａｎｓｐｏｒｔｏｆｍｙｏｃａｒｄｉａｌｍｉｔｏｃｈｏｎｄｒｉａｄｕｒｉｎｇｔｈｅｅａｒｌｙｓｔａｇｅｏｆｃｏｍｂｉｎｅｄｒａｄｉａｔｉｏｎ－ｂｕｒｎｉｎｊｕｒｙｉｎｒａｔｓ￥（熊业）（陈宗荣）ＸｉｏｎｇＹｅ；Ｃｈｅｎ...     

A study on mitochondrial respiratory dysfunction and lipid peroxidation in the liver after radiation,burn,and combined radiation-burn injuries in mice

Male mice were subjected to 6 Gy total body irradiation,20% TBSAfull-thickness burns,or combined radiation-burn injury and lipid peroxides(LPO),vita-min E,sulfhydryl group,respiratory control ratio(RCR),ADP/O ratio,and cytochromeoxidase activity of the liver mitochondria were determined in the first 9 d postinjury.Theresults are as follows:(1)LPO level increased in the early postinjury stage after combinedradiation-burn injury,on the 5th-7th day after irradiation and on the 7th day postburn.(2)Vitamin E level decreased significantly in the two groups of radiation and burn inju-ries but showed no significant decrease after combined injury.(3)The sulfhydryl groupshowed a tendency to increase in all the 3 groups.(4)The activity of cytochrome oxidaseincreased significantly on the 7th day after radiation but decreased considerably in theburn and combined injury groups.(5)RCR and ADP/O ratio decreased more significantlyin the combined injury group than in either the radiation group or the burn group.These facts suggest that the respiratory dysfunction of the liver mitochondria results mostprobably from the damage on the mitochondrial membrane due to lipid peroxidation.     

烧伤及烧伤焦痂脂类对大鼠肝线粒体脂肪酸含量的影响

我们用气相色谱法分析了15％Ⅲ度烧伤、烧伤焦痂脂类(D1)和正常皮肤脂类(D’1)对大鼠肝线粒体脂肪酸含量的影响。结果表明烧伤后第7天大鼠肝线粒体不饱和脂肪酸含量显著下降和饱和脂肪酸含量上升。D1和D’1能使孵育体系中离体肝线粒体不饱和脂肪酸含量下降和饱和脂肪酸含量上升,但D1的作用大于D’1,使不饱和脂肪酸含量下降更明显。本文讨论了烧伤后和D1及D’1使线粒体不饱和脂肪酸含量下降的可能机理。     

Protective effects of verapamil on myocardial mitochondrial respiratory function after combined radiation－burn injury in rats

Ｐｒｏｔｅｃｔｉｖｅｅｆｆｅｃｔｓｏｆｖｅｒａｐａｍｉｌｏｎｍｙｏｃａｒｄｉａｌｍｉｔｏｃｈｏｎｄｒｉａｌｒｅｓｐｉｒａｔｏｒｙｆｕｎｃｔｉｏｎａｆｔｅｒ　ｃｏｍｂｉｎｅｄｒａｄｉａｔｉｏｎ－ｂｕｒｎｉｎｊｕｒｙｉｎｒａｔｓＸｉｏｎｇＹｅ（熊业）；Ｃ...     

放烧复合伤早期心肌能量代谢变化

文中报告了６Ｇｙ放射损伤合并３０％Ⅲ度烧伤后４８ｈ内大鼠心肌线粒体氧化磷酸化功能和肌球蛋白ＡＴＰ酶活力变化与补液的作用。结果表明６Ｇｙ全身照射、３０％Ⅲ度烧伤和放烧复合伤后心肌线粒体氧化磷酸化功能和肌球蛋白ＡＴＰ酶活力显著下降，放烧复合伤时下降更严重。这些变化与伤后动脉血压下降关系十分密切。补液显著改善放烧复合伤后心肌的能量代谢。这些结果揭示缺血缺氧是心肌能量代谢障碍的重要诱因。     

放射损伤,烧伤及复合伤时肝线粒体功能障碍与脂质过氧化作用

本文采用小鼠6 Gyγ射线全身照射和20％Ⅲ度凝固汽油烧伤模型,观察了伤后9 d内单伤和复合伤小鼠肝线粒体脂质过氧化物、维生素E及巯基含量、细胞色素氧化酶活力,呼吸控制率和磷氧比值的变化。结果表明脂质过氧化作用在复合伤组早期增加呈现复合效应。维生素E含量在单伤组下降更明显。巯基含量在各伤组变化总趋势是升高。细胞色素氧化酶活力在单放组第7天显著升高。呼吸控制率和磷氧比值以复合伤组下降更明显。实验结果提示伤后肝线粒体呼吸功能障碍与脂质过氧化损伤有一定关系。     

氢过氧化枯烯引起离体线粒体脂质过氧化作用的初步研究

氢过氧化桔烯(CHP)能非常显著地引起小鼠肝离体线粒体脂质过氧化作用(LP)、CHP在低浓度时.脂质过氧化物(LPO)与CHP浓度成线性增加,CHP在150μmol/L时,LPO形成达最大.-LPO与温育时间(20 mim内)成线性增加.以后保持在较高不变水平.还原型谷胱甘肽(GSH),维生素E(Vit.E)和依地酸(EDTA)能非常昱著地抑制CHP引起的线粒体LP而且GSH含量减少,Vit.E几乎不变.用二甲基亚砜(DMSO)和超氧化物岐化酶(SOD)排除了OH-及O2-的形成.本文讨论了CHP引起LP的可能机理及抗氧化剂的作用.