EFFECTS OF S86019, AN ACTIVE COMPONENT FROM PURALIA LOB AT A, ON CELL DIFFERENTIATION AND CELL CYCLE TRAVERSE OF HL-60 CELLS

The effects of S86019, an active component from Puralia lobata, on the induction of cell differentiation and cell cycle traverse of HL-60 cells were described. It was shown that cell proliferation of HL-60 cells was inhibited by S86019 in vitro. Under the action of S86019 the HL-60 cells were induced to differentiate into metamyelocytes, myelocytes and much matured cells with banded or segmented nucleus. Flow cytometry demonstrated that the cell population of HL-60 cells was blocked at G1 phase which resulted in the elevation of percentage of G1 cells and decrease of percentage of cells in S phase. Experimental results demonstrated that S86019 is an active inducer of cell differentiation in HL-60 cells.     

ACM B和ABM A对人早幼粒白血病细胞分化诱导作用的比较

本文对蒽环类抗瘤抗生素阿克拉霉素B和阿克拉霉素A对人早幼粒的白血病细胞(HL-60)的分化诱导作用进行了比较,HL-60细胞经ACM B和ACMA处理后,均能明显抑制其细胞的增殖,并能明显分化诱导HL-60细胞。其抑制和分化诱导程度均均随浓度的增加而增强,但ACMB对HL-60细胞的抑制和分化诱     

癌化学预防药维胺酸的药理学研究

维胺酸(N-4-(hydroxycarbophenyl)-retinamide;RⅡ)在30～50mg/kg的剂量下对大鼠移植性软骨肉瘤有明显治疗作用。 低浓度的RⅡ对HL-60细胞有较弱的分化诱导活性,同时加入S86019可使其活性增强。流式细胞光度术分析表明,RⅡ可使HL-60细胞产生G_1期阻断,与S86019联合应用有良好协同阻断效应,使90％细胞积聚在G_1期,Northern blot分析表明,用RⅡ和S86019联合处理HL-60细胞12h后,c-myc表达明显受到抑制,与DNA合成密切相关的胸腺嘧啶核苷酸合成酶mRNA表达也受到抑制。     

新维甲类化合物—R—81001对HL—60细胞的分化诱导作用

应用软琼脂集落形成法选出单克隆HL-60细胞系,系统地研究新维甲类化合物R81001的分化诱导作用。证明该药可使人早幼粒白血病细胞的NBT还原能力明显增强,呈浓度依赖性。形态学观察表明,在药物作用下细胞的核浆比例降低,核仁减少或消失.胞浆中嗜天青颗粒减少,出现特异性颗粒。多数细胞处于中或晚幼粒细胞阶段。非特异酯酶染色试验证明,R81001诱导HL-60细胞沿粒系统定向分化。