肝再生磷酸酶-3小干扰RNA对异位子宫内膜间质细胞迁移的影响

目的 通过siRNA体外沉默肝再生磷酸酶-3(PRL-3),探讨其对异位子宫内膜间质细胞迁移的影响.方法 原代培养异位子宫内膜间质细胞,运用siRNA技术干扰沉默PRL-3基因,Western blot检测干扰后PRL-3蛋白表达变化,观察细胞板状伪足的形成,采用划痕实验、transwell细胞迁移实验观察并比较细胞迁移能力的改变.结果 经siRNA 干扰沉默后实验组PRL-3蛋白相对表达量较对照组明显下降(P<0.05),细胞形成的板状伪足与对照组相比明显减少,相同时间内发生定向迁移的实验组细胞数为0.87±0.21,空白对照组为1.75±0.28,阴性对照组为1.63±0.39,前者与后两者比较差异有统计学意义(P<0.05).结论 PRL-3 siRNA能有效沉默PRL-3基因,并且使异位子宫内膜间质细胞的迁移能力下降.

Abstract：

Objective To evaluate the effects of PRL-3 siRNA (small interfering RNA) on the migration of endometriotic stromal cells.Methods Primary endometriotic stromal cells were cultured in vitro.Then PRL-3 (phosphatase of regenerating liver-3) siRNA was transfected to silence the PRL-3 gene.And the PRL-3 protein expression was analyzed by Western blot.The changes of cell migration were detected by cell pseudopod formation, scratch test and transwell cell migration test.Results The expression of PRL-3 protein significantly decreased in the experimental group versus two other control groups (P<0.05).The formation of cell pseudopod was much less in experimental group than that in control groups. Within the same time, the number of migration cells was 0.87±0.21 in experimental group.It was less than 1.75±0.28 in blank control group and 1.63±0.39 in negative control group(P<0.05).Conclusion PRL-3 siRNA can down-regulate the PRL-3 gene and decrease the migratory capacity of endometriotic stromal cells.And PRL-3 may be a promising target in the therapeutics of endometriosis.     

邯郸市124家美容理发店卫生现状调查

为了解本市美容理发业卫生现状,我们于1995年冬季对我市124家美容理发店进行了调查,结果如下;1.对象和方法以本市124家理发、美容店对调查对象,按照卫生部颁布的《公共场所卫生监测要点》和国家《理发美容店卫生标准》(GB9666—88),对持证情况、室内采光、空气质量、理发用毛巾、剪刀、胡刷的大肠菌群、金黄色葡萄球菌等进行了监测.     

微机计算外照射预置剂量

目的 利用微机计算肿瘤外照射任意照射野的预置剂量,减少临床剂量计算误差,保证放射治疗质量,实现预置剂量的微机化。方法 采用ＱＢＡＳＩＣ语言编程,把算量中的各个参数设入,并输入已测的ＴＭＲ、ＦＦ、Ｆｗ、Ｆｔ的值,产生可运行的算量模式,把微机与手工计算的结果进行对照。结果 微机算量精度提高,效率提高５倍（规则野）及７．５倍（不规则野）。结论 微机算量优于传统手术计算,特别是不规则照射野。