全文获取类型
收费全文 | 1335篇 |
免费 | 166篇 |
国内免费 | 279篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 10篇 |
妇产科学 | 23篇 |
基础医学 | 187篇 |
口腔科学 | 5篇 |
临床医学 | 62篇 |
内科学 | 223篇 |
皮肤病学 | 9篇 |
神经病学 | 108篇 |
特种医学 | 19篇 |
外科学 | 133篇 |
综合类 | 406篇 |
现状与发展 | 12篇 |
一般理论 | 2篇 |
预防医学 | 48篇 |
眼科学 | 127篇 |
药学 | 185篇 |
2篇 | |
中国医学 | 78篇 |
肿瘤学 | 130篇 |
出版年
2024年 | 25篇 |
2023年 | 107篇 |
2022年 | 55篇 |
2021年 | 67篇 |
2020年 | 18篇 |
2019年 | 19篇 |
2018年 | 28篇 |
2017年 | 21篇 |
2016年 | 22篇 |
2015年 | 44篇 |
2014年 | 70篇 |
2013年 | 77篇 |
2012年 | 151篇 |
2011年 | 193篇 |
2010年 | 30篇 |
2009年 | 28篇 |
2008年 | 142篇 |
2007年 | 131篇 |
2006年 | 131篇 |
2005年 | 93篇 |
2004年 | 65篇 |
2003年 | 59篇 |
2002年 | 40篇 |
2001年 | 29篇 |
2000年 | 38篇 |
1999年 | 24篇 |
1998年 | 18篇 |
1997年 | 19篇 |
1996年 | 4篇 |
1995年 | 12篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1984年 | 2篇 |
1983年 | 1篇 |
排序方式: 共有1780条查询结果,搜索用时 46 毫秒
1.
MA Zhi Tao WANG Na HE Fan LIU Xiao Feng SHEN Xin 《Biomedical and environmental sciences : BES》2022,35(5):458-462
<正>Mycobacterium tuberculosis is the primary bacteria that leads to human tuberculosis(TB),which usually affects the pulmonary organs. TB is one of the top 10 causes of death worldwide;however, China is one of eight countries that together account for two-thirds of the total number of cases globally. 相似文献
2.
Simultaneous Determination of Six Compounds in Rat Plasma by Ultra‑Performance Liquid Chromatography with Tandem Mass Spectrometry: Application in the Pharmacokinetic Study of Qing Gan‑Shu Yu‑Fang 下载免费PDF全文
Hai Jiang A‑Jiao Hou Yan‑Yan Zhang Wen‑Jing Man Liu Yang Yong‑Hai Meng Xin‑Yue Guo Song Wang Jia‑Xu Zhang Bing‑You Yang Qiu‑Hong Wang Kelvin Chan Hai‑Xue Kuang 《World Journal of Traditional Chinese Medicine》2019,(4)
A rapid and high selective ultra?performance liquid chromatography (UPLC) with tandem mass spectrometry method for simultaneous
determination of six compounds including albiflorin, paeoniflorin, picroside I, picroside II, saikosaponin A, and saikosaponin D in rat plasma
was developed and validated using butyl p-hydroxybenzoate as an internal standard. One-step direct protein precipitation with acetonitrile was
used to extract the compounds from the rat plasma samples. Chromatographic separation was achieved using an ACQUITY UPLC BEH C18
column (100 mm × 2.1 mm, 1.7 µm) at a flow rate of 0.4 mL/min, using gradient mode containing 0.1% formic acid in water and acetonitrile
were used as the Mobile phase A and B. Electrospray ionization in negative ion mode and multiple reaction monitoring were used to identify
and quantify active components. Calibration curves showed good linearity (R2 > 0.9908) over a wide concentration range for all compounds.
The intra- and interday precision (relative standard deviation) ranged 2.4%–7.0% and 2.6%–8.0%, respectively. The accuracy (relative error)
was from ?13.0% to 13.2% at all quality control levels. The recovery ranged from 81.1% to 92.5%. The validated method was successfully
applied to pharmacokinetic study in rats after oral administration of Qing Gan?Shu Yu?Fang. The results show that one can draw a conclusion
that these six active ingredients can be quickly absorbed and play a pharmacodynamic role rapidly in vivo. 相似文献
3.
Xiangyu Piao Changkai Sun Zhe Jin Jing Yu Xiaodan Chang Xin Tang Hongmei Liu Peiyang Zhou 《中国神经再生研究》2007,2(9):540-543
BACKGROUND: The cerebral ischemia and ischemia/reperfusion animal models are used to simulate the human cerebrovascular diseases is one of the popular topics of neurological science recently. To study the pathophysiology, pathogenesis, prophylaxis and treatment of ischemic cerebrovascular diseases and to establish the ideal animal model that is the most similar to the human cerebral ischemia, are the topics that the people generally cared about.
OBJECTIVE: To evaluate the effects of aerocyst-blocking bilateral ascending pharyngeal artery on the establishment of cerebral ischemia models by using digital subtraction angiography (DSA), magnetic resonance diffusion-weighted imaging (DWI) and magnetic resonance perfusion-weighted imaging (PWI).
DESIGN: Repetitive measure animal experiment.
SETTING: Zhongshan Hospital Affiliated to Dalian University.
MATERIALS: The experiment was carried out in the Animal Laboratory (Provincial Laboratory), Zhongshan Hospital of Dalian Univeristy from January to May 2006. A total of 14 domestic piglets, of 6 months old, weighing 12–15 kg, of either gender, were selected from Animal Experimental Center, Dalian University. Multistar T.O.P digital subtraction angiography machine was provided by Siemens Company, German.
METHODS: Aerocyst-blocking bilateral ascending pharyngeal artery was used to establish cerebral ischemia models. And then, Multistar T.O.P. DSA was used for imaging of cerebral vessels before blocking, during blocking and at 0.5 and 2 hours after ischemia perfusion. GE Signa 1.5 T supraconduction magnetic resonance imaging was used for DWI examination; in addition, PWI was used based on focal sites and areas. Otherwise, magnetic resonance imaging (MRI) was used to detect signal changes of T1WI and T2WI in ischemic areas.
MAIN OUTCOME MEASURES: Analytic results of DSA, DWI, PWI and MRI.
RESULTS: All 14 experimental piglets were involved in the final analysis. ① DSA: The blood flow of bilateral ascending pharyngeal arteries and its branch were blocked at blocking phase, which restored 0.5 and 2 hours after reperfusion. ② DWI and PWI: There were no observable abnormalities in PWI and DWI at pre-blocking. Abnormal increased signals were found on both DWI and PWI at during and post-blocking. There were reduction in ADC and rCBF and delay in rTTP at all time points except pre-blocking. ③ MRI: There were no abnormal signals observable at any time of pre- and post-blocking in T1WI and T2WI.
CONCLUSION: It is feasible to establish this kind of animal experimental models, and it can simulate the ischemic state; meanwhile, the existence and extent can be showed directly by DSA, DWI, and PWI. 相似文献
4.
Hongning Zhao Xiaoming Wang Junqiang Zhang Deben Yang Xiaoqiong Zhao Xin Liu Hui Huang Jianxiu Hu 《中国神经再生研究》2007,2(11):675-677
BACKGROUND: The examination of sympathetic skin response is an important index for assessing the autonomic nerve function, and patients with myasthenia gravis are always accompanied by dysautonomia. Therefore, it will be important to know whether sympathetic skin response can be used as the index for the clinical evaluation of myasthenia gravis.
OBJECTIVE: To investigate the diagnostic value of sympathetic skin response in the damage of autonomic nerve function of patients with myasthenia gravis.
DESIGN: A case-controlled comparative observation.
SETTING: Department of Neurology and Room of Nerve Electromyogram, the Affiliated Hospital of North Sichuan Medical College.
PARTICIPANTS: Thirty outpatients or inpatients with myasthenia gravis were selected from the Department of Neurology, the Affiliated Hospital of North Sichuan Medical College from May 2006 to May 2007, including 9 males and 21 females, aged 8–72 years with a mean age of (28±5) years old. They were all accorded with the diagnostic standards of myasthenia gravis, accompanied by different severity of autonomic nerve symptoms, including poor skin nutrition, sweating of hands and feet, pyknocardia, persistent hypotension, abdominal pain, constipation, etc. They all had not taken any drug affecting the autonomic nerve function before the examination. Informed consents were obtained from all the patients. Meanwhile, 30 healthy physical examinees were enrolled as the normal control group, including 10 males and 20 females, aged 10–75 years with a mean age of (31±5) years old. Approval was obtained from the hospital ethic committee.
METHODS: After admission, the patients were examined with sympathetic skin response using DANTEC keypoint 2.0 electromyography evoked potential apparatus (Danmark). The changes of the latency and wave amplitude of sympathetic skin response were observed. The subjects in the normal control group were examined with the same methods at physical examination. Abnormality was judged by the disappearance of wave form, latency longer than that in the normal control group by Mean±2.5SD, or wave amplitude lower than the average value in the normal control group by 50%.
MAIN OUTCOME MEASURES: The results of the latency and wave amplitude of sympathetic skin response were compared between the patients with myasthenia gravis and normal controls.
RESULTS: All the 30 patients with myasthenia gravis and 30 healthy physical examinees were involved in the final analysis of results. There were no significant differences between the left and right upper and lower limbs in both the myasthenia gravis group and normal control group (P > 0.05). In the myasthenia gravis group, the abnormal rate of sympathetic skin response was 37% (11/30), the latency was prolonged and the wave amplitude was decreased as compared with those in the normal control group, and there were significant differences (P < 0.01).
CONCLUSION: Sympathetic skin response can be used as an electrophysiological index for judging the damages of autonomic nerve function in patients with myasthenia gravis. 相似文献
5.
睾丸网注射法建立转基因小鼠 总被引:1,自引:0,他引:1
目的 为了研究睾丸网注射法建立转基因小鼠的可行性。方法 本试验应用显微注射仪将脂质体包裹的质粒pEGFP—N1通过睾丸网注射到4周龄昆明(KM)小白鼠睾丸生精小管内。注射后6周,与自然发情雌鼠交配,用PCR法检测仔鼠基因组中的外源DNA。结果 共注射4只雄性小鼠,3只存活,并且具有交配,受精能力,共获仔鼠63只,其中2只为PCR阳性仔鼠。结论 用睾丸网注射法制备转基因动物是可行的。 相似文献
6.
Takada D Emi M Ezura Y Nobe Y Kawamura K Iino Y Katayama Y Xin Y Wu LL Larringa-Shum S Stephenson SH Hunt SC Hopkins PN 《Journal of human genetics》2002,47(12):0656-0664
7.
Jiang S Xin R Wu X Lin S Qian Y Ren D Tang G Wang D 《American journal of medical genetics》2000,96(3):289-292
Attention deficit hyperactivity disorder (ADHD) is a prevalent disorder in children. The etiology of this disease is not clear. Genetics studies have suggested the involvement of the dopamine DRD-4 receptor gene and dopamine transporter gene (DAT1). Clinical studies have shown that monoamine oxidase-B (MAO-B) inhibitors are effective in the treatment of ADHD. These findings suggest that monoamine oxidase (MAO) genes might be involved in the origin of ADHD. In the present work, the DXS7 locus of chromosome X, which is closely linked to MAO genes, was selected as a marker to study the possible association between ADHD and MAO genes in the Chinese population. Haplotype-based haplotype relative risk (HHRR) and the transmission disequilibrium test (TDT) methods were employed to analyze the association and the linkage disequilibrium, respectively. Significant association (X(2) = 15.86; 1 df; P < 0.001) and linkage (X(2) = 14.88; 1 df; P < 0.001) were detected between the 157-bp allele of the DXS7 locus and the DSM-III-R-diagnosed ADHD (N = 72) in trios composed of father, mother, and affected offspring. The data suggested that ADHD was associated and in linkage with DXS7 locus. 相似文献
8.
9.
Antitumor immune response by CX3CL1 fractalkine gene transfer depends on both NK and T cells 总被引:6,自引:0,他引:6
Xin H Kikuchi T Andarini S Ohkouchi S Suzuki T Nukiwa T Huqun Hagiwara K Honjo T Saijo Y 《European journal of immunology》2005,35(5):1371-1380
The CX3C chemokine fractalkine (CX3CL1) exists as both a membrane-bound form promoting firm cell-cell adhesion and a soluble form chemoattracting leukocytes expressing its receptor CX3CR1. When adenoviral vector expressing mouse fractalkine (AdFKN) was transduced to the tumor cells, fractalkine was expressed as both membrane-bound form on the tumor cells and soluble form in the supernatant in vitro. Intratumoral injection of AdFKN (1 x 10(9)PFU/tumor) into C26 and B16F10 tumors resulted in marked reduction of tumor growth compared to control (C26: 86.5%, p<0.001; B16F10: 85.5%, p<0.001). Histological examination of tumor tissues revealed abundant infiltration of NK cells, dendritic cells, and CD8(+) T lymphocytes 3 and/or 6 days after treatment with AdFKN. Splenocytes from mice treated by AdFKN developed tumor-specific cytotoxic T cells, and thereby protected from rechallenging with parental tumor cells. Antitumor effects by AdFKN were completely abrogated in both NK cell-depleted mice and CD8(-/-) mice, and partially blocked in CD4(-/-) mice. These data indicated that fractalkine mediates antitumor effects by both NK cell-dependent and T cell-dependent mechanisms. This study suggests that fractalkine can be a suitable candidate for immunogene therapy of cancer because fractalkine induces both innate and adaptive immunity. 相似文献
10.
VALUE AND LIMITATIONS OF SINUS NODE FUNCTION TE.ST
BY ATRIAL PACING IN SICK SINUS SYNDROME DIAGNOSIS
Sun Rui-long孙瑞龙 Chen Xin陈新 Wang Fang-zhengHu Sheng-jun胡绳俊 Tian Rui-guo田瑞国and Qi Lian-xiaClinical Electrophysiology Laborato''ry Institute of Cardiovascular Diseases Chinese Academy of Medical Sciences Beijing 《中华医学杂志(英文版)》1984,97(2):142-146
In order to evaluate the significance of the
sinus node function test by transvenous atrial
pacing in the diagno,sis of sick sinus syndrome
(SSS), sinus node recovery time (SRT), co]rrected
sinus node recovery time (CSRT) and total atrio-
sinus and sino-atrial conduction time (SACT)
are observed in 69 patients including non-SSS
group 39 cases and SSS group 30 cases.
According to our data, we suggest criteria
for sinus node dysfunction as follows. SRT>1,400
ms and/or A-V junctional esca.pe before sinus
recovery and;or secondary pause, CSRT>560 ms.
total SACT>300 ms. With l item abnormal, the
false positive rat.e in the non-SSS group is 7.7c7。
and the false negative rate in the S.SS group 3.30i"
with 2 items abnormal, the false positive rate in
the non-SSS group Oi and the false negative
rate in the SSS grOiup 6.7'70.
Atropine test results are compared with that
of atrial pacing, 95.8To and 84.2% are compatible
with each other in these 2 groups. In order to
cut the use of invasive technic to the minimum,
we propose performing the atropine test first.
Only in those with unexplainable test results
should the trial pacing test. be resorted to.. 相似文献