“献方运动”视阈下的广州中医学院教职工献方研究

广州中医学院教职工献方是20世纪50、60年代"献方运动"的产物;按临床各科编撰,条理清晰,理法俱全;邓铁涛、罗元恺、陶葆荪、何汝湛、李仲守、黎炳南、沈炎南、刘赤选等当代岭南名老中医的献方,占教职工献方的66. 6%,所有献方标明用量、煎药法、服用法、主治,一些献方还说明出处与运用经验;传染病献方较多与当时的社会历史背景有关;部分献方至今仍被运用于临床;广州中医学院教职工献方属献方中的"珍品"。     

全蝎白术白头翁混合发酵品不同极性部位体外抗病毒研究

目的探讨全蝎白术白头翁混合发酵品(SAPHFP)不同极性部位体外抗病毒作用及可能活性成分的含量。方法对SAPHFP分别采用超声水提、50%、80%乙醇回流提取,提取物通过MTT法分别对RSV、EV71、COX-B2、HSV四种病毒株进行体外抗病毒作用的实验;采用Lowry法、苯酚-硫酸法及NaNO_2-AlCl_3-NaOH比色法分别测定三种极性部位中的总蛋白、总多糖及总黄酮的含量。结果 SAPHFP三种提取成分中,50%乙醇提取物对病毒的抑制率最好,尤其是对EV71和HSV病毒,TI指数分别为46.30、56.82。三者的体外抗病毒有效率均高于阳性对照药利巴韦林;SAPHFP的50%乙醇提取液与未发酵品比较,感染了HSV病毒的正常细胞其存活率显著提高;水提液中总蛋白、总多糖、总黄酮含量最高,分别为:177.1269、517.7191、9.624298。结论 SAPHFP水提、50%、80%乙醇提取物体外对四种病毒均有一定的抑制作用,体外抗以上病毒株的活性与供试品中所含的总蛋白、总多糖、总黄酮含量不完全成对等关系。     

氯霉素眼药水的新用

氯霉素眼药水是治疗沙眼和细菌性结膜炎、角膜炎的常用药,近年来人们发现它还有其他作用。 淋病 用0.25％～0.4％氯霉素眼药水1支,早晚各1次,男性于尿道口注入半支;女性于阴道口注入半支。每次用     

猪苓汤加减治疗原发性肾性血尿的体会

血尿是临床常见症状 ,大部分是慢性肾炎的表现之一 ,如欲将每一位病例血尿的病因病原查清楚 ,并非易事 ,因此作者仅将猪苓汤加减治疗原发性肾性血尿的体会叙述如下 ,供读者参考。资料与方法1　诊断标准　 (1)中医 :小便短赤带血 ,并有灼热涩痛感 ,浮肿 ,少尿 ,或伴腰膝酸软 ,头晕耳鸣 ,心烦不眠等阴虚见证。舌质红少苔 ,脉细数。诊断要点 :小便带血 (包括镜下血尿 )及舌苔脉象。 (2 )西医 :肉眼血尿或镜下血尿 ,红细胞数≥ 3个 /HP。尿潜血 +以上。符合叶任高第 5版《内科学》慢性肾小球肾炎诊断标准 ,排除继发性肾脏疾病。2　一般资料　本…     

补肾活血方药对慢性再生障碍性贫血患者骨髓细胞体外影响的实验研究

目的　研究补肾活血方药对慢性再生障碍性贫血 (CAA)患者骨髓细胞体外增殖的影响 ,探讨其治疗CAA的机制。方法　采用细胞培养 ,光镜下观察补肾活血中药作用于CAA患者骨髓细胞后的形态学 ;3 H TdR掺入和液体闪烁技术方法 ,检测补肾活血中药对CAA患者骨髓细胞的增殖变化。结果　加入补肾活血中药组 ,CAA患者骨髓细胞培养第 4 8小时见细胞丛明显增多 ,72h见大量集落形成 ;3 H TdR掺入法显示 ,补肾活血中药组骨髓细胞的增殖 ,以每孔 75g L最有效。在 5 0～ 10 0g L范围内 ,与对照组比较差异有显著性 (P <0 .0 1) ;2 5g L和 12 5g L组与对照组比较差异无显著性 (P <0 .0 5 )。结论　补肾活血方药在体外有促进CAA患者骨髓细胞增殖的作用 ,为补肾活血中药治疗CAA提供了实验与理论依据     

广东省β珠蛋白基因簇RFLP分析及β地中海

RFLP haplotypes of 69 chromosomes from members of 18 families affected with beta-thalassemia (beta T) in Guangdong Province were analyzed. 17 haplotypes were found. Haplotypes 1, 2 and 3 accounted for most of them and 4 new haplotypes were identified, three of which were associated with beta T genes. In 9 families, the haplotype data could be used for definitive prenatal diagnosis. In 7 families, 50% exclusive diagnosis could be achieved. In order to know the frequencies of various beta T genes in Guangdong Province and to improve prenatal diagnosis, we identified the beta T genes of 46 affected children in Guangdong Province by amplifying beta-globin gene sequences with the polymerase chain reaction (PCR) and hybridization with allele specific oligonucleotide (ASO) probes. 82 beta T genes hybridized with 6 probes. The most common beta T mutations were frameshift 41/42-TCTT, -28 A----G and IVS-2 nt654 G----T, accounting for 80% of the total. In 36 families PCR combined with hybridization using 6 ASO probes could provide definitive prenatal diagnosis.     

Treatment of Motor Neuron Disease with Qi-invigorating Herbs—— A Report of 31 Cases

The motor neuron disease (MND) refers to a group of progressive diseases with unknown reasons, which attacks the cells of the anterior horn of the spinal cord, the motor nuclei of the brain stem cranial nerves and the pyramidal cells of the cerebral motor cortex. It is characterized in clinic by atrophy of the muscles, myasthenia and even death due to paralysis of the respiratory muscle. Currently, there is still no any effective cure for this illness. 50-70% of the victims will die in 3 to 5 years, and the survival time for those with brain stem injuries is no more than two years.1 Since 1996, the authors have treated 31 cases of motor neuron disease with large dosage of qi-invigorating drugs in accordance with Prof. Liu Mocai's experience, and obtained certain therapeutic effects. A report follows.